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Temperature-dependent DNA binding of DicA protein in vivo and in vitro

In vivo와 in vitro에서 DicA 단백질의 온도 의존적 DNA 결합

Lee, Yonho;Yun, Sang Hoon;Lim, Heon M.
이연호;윤상훈;임헌만

  • Received : 2019.02.27
  • Accepted : 2019.06.26
  • Published : 2019.09.30

Abstract

In Escherichia coli, DicA protein is involved in cell division control. DicA protein is known to bind DNA better at $25^{\circ}C$ than at $37^{\circ}C$. However, the molecular cause of the temperature dependent binding is not clear. In this study, we investigated how DicA binds DNA and why its DNA binding activity depends on temperature. An unique in vivo DNA binding assay developed in this laboratory showed that unlike the homologous proteins such as RovA or SlyA, DicA uses its N-terminal domain for DNA binding. The in vivo DNA binding assay of DicA also demonstrated that the temperature-dependent DNA binding activity does not come from Cnu or H-NS that is known to bind DNA better at $25^{\circ}C$ than at $37^{\circ}C$. Electrophoretic Mobility Shift Assay (EMSA), when performed with purified DicA protein, did not show temperature-dependent DicA binding activity. However when EMSA was performed with crude protein from WT E. coli cells, temperature-dependent DicA binding activity was observed, suggesting that there is a factor(s) that confers temperature DNA binding activity of DicA in vivo.

Keywords

Escherichia coli;DicA;DNA binding;filamentous growth;temperature-dependent DNA binding activity

Acknowledgement

Supported by : 충남대학교