Antimicrobial Activity of Prodigiosin from Serratia sp. PDGS120915 Against Intestinal Pathogenic Bacteria

  • Ji, Keunho (Basic Science Research Institute, Pukyong National University) ;
  • Kim, Young Tae (Department of Microbiology, Pukyong National University)
  • Received : 2019.01.18
  • Accepted : 2019.02.11
  • Published : 2019.09.28


This study aimed to identify and characterize the antimicrobial activity of prodigiosin produced by Serratia sp. $PDGS^{120915}$ isolated from stream water in Busan, Korea; the identification was performed using phonological, biochemical, and molecular techniques, including 16S rRNA sequence analysis. Prodigiosin from the bacterial culture was purified by high-performance liquid chromatography (HPLC), and its antimicrobial activity and minimum inhibitory concentrations (MICs) were evaluated against 10 intestinal pathogenic gram-positive and negative bacteria. The results revealed that the isolated prodigiosin exhibited high antimicrobial activity against Listeria monocytogenes, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, and Vibrio parahaemolyticus; further, the isolated prodigiosin showed minimum inhibitory concentrations (MICs) between $3{\mu}g/ml$ and 30 mg/ml, but they were not active against Bacillus subtilis, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli. In conclusion, prodigiosin isolated from Serratia sp. $PDGS^{120915}$ showed high antimicrobial activity against intestinal pathogenic bacteria and has potential applications in the development of new antimicrobial agents.


Prodigiosin;antimicrobial;pigment;Serratia;FIC index


Supported by : Pukyong National University


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