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Exploration of β-Glucuronidase Activity of Lactic Acid Bacteria Isolated from Kimchi

김치에서 분리된 젖산균의 β-glucuronidase 활성 탐색

  • Kim, Eun-Jung (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Shin, In-Ung (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Kwun, Se-Young (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Park, Eun-Hee (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Yi, Jae-Hyoung (Ginseng and Medicinal Plant Research Institute, Gangwon Agricultural Research & Extension Services) ;
  • Kim, Myoung-Dong (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
  • 김은정 (강원대학교 바이오산업공학부) ;
  • 신인웅 (강원대학교 바이오산업공학부) ;
  • 권세영 (강원대학교 바이오산업공학부) ;
  • 박은희 (강원대학교 바이오산업공학부) ;
  • 이재형 (강원도농업기술원 인삼약초연구소) ;
  • 김명동 (강원대학교 바이오산업공학부)
  • Received : 2019.01.07
  • Accepted : 2019.02.27
  • Published : 2019.09.28

Abstract

Lactic acid bacteria (LAB) isolated from kimchi were studied for their ${\beta}$-glucuronidase activity. Among the 156 strains tested, 52 strains utilized glucuronic acid as a carbon source and their intracellular ${\beta}$-glucuronidase activities were significantly higher than their extracellular activities. Leuconostoc mesenteroides KFRI 73007 isolated from turnip kimchi exhibited the highest intracellular ${\beta}$-glucuronidase activity of $0.77{\pm}0.01U/mg$ protein, which was further increased to $1.14{\pm}0.01U/mg$ protein under optimized reaction conditions (pH 7, $37^{\circ}C$). The activity of ${\beta}$-glucuronidase was notably decreased by the addition of divalent cations, and glucuronic acid was the best carbon source to produce ${\beta}$-glucuronidase in Leu. mesenteroides KFRI 73007.

Keywords

${\beta}$-glucuronidase;Kimchi;Lactic acid bacteria;Leuconostoc mesenteroides

Acknowledgement

Supported by : Korea Institute for Advancement of Technology (KIAT)

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