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Characterization of Alpha-Amylase from Aspergillus niger Aggregate F Isolated from a Fermented Cassava Gatot Grown in Potato Peel Waste Medium

  • Angelia, Cindy (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Sanjaya, Astia (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Aida, Aida (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Tanudjaja, Ellen (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Victor, Hans (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Cahyani, Antari Daru (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Tan, Tjie Jan (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan) ;
  • Pinontoan, Reinhard (Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan)
  • Received : 2018.11.30
  • Accepted : 2019.02.18
  • Published : 2019.09.28

Abstract

The use of GRAS microorganisms isolated from fermented foods during amylase production using an economical food-waste medium provides more opportunities to produce amylase with a wider range of applications. Hence, this study aimed to isolate a good amylase-producing fungi from the traditional Indonesian fermented cassava, gatot, and to identify the amylase-producing capability of the isolate in a potato peel waste (PPW) medium. Black-colored fungi isolated from gatot was morphologically identified and the amylase produced was characterized using SDS-PAGE and Native PAGE. The isolate was then grown on PPW medium, and the amylase produced was further characterized. Morphological identification and enzyme characterization revealed that the Aspergillus niger aggregate F isolated from gatot secreted an active extracellular ${\alpha}$-amylase with an optimum pH of 5-6. In conclusion, Aspergillus niger aggregate F isolated from gatot can be used to produce ${\alpha}$-amylase using PPW as a medium.

Keywords

Alpha-amylase;Aspergillus niger;fermented cassava gatot;potato peel waste

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