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Inhibition of Nelumbo nucifera Stamens-derived Kaempferol on FcεRI-mediated GATA-1 Expression

  • Shim, Sun-Yup (Department of Aqualife Science, College of Fisheries and Ocean Sciences, Chonnam National University)
  • Received : 2019.04.29
  • Accepted : 2019.05.28
  • Published : 2019.09.28

Abstract

The transcription factor, GATA-1, plays an important role in the $Fc{\varepsilon}RI$ ${\alpha}$ chain expression in mast cells and basophils. This study was conducted to investigate the downregulation of the transcription factor GATA-1 by kaempferol isolated from Nelumbo nucifera stamens in $Fc{\varepsilon}RI$-mediated allergic reactions. Kaempferol inhibited $Fc{\varepsilon}RI$-mediated histamine release. Western blotting analysis and RT-PCR showed that the protein and mRNA expression of GATA-1 was suppressed by kaempferol in a dose-dependent manner. These results suggest that kaempferol may inactivate basophils by downregulating the $Fc{\varepsilon}RI$ ${\alpha}$ chain expression via the inhibition of the GATA-1 expression.

Keywords

Kaempferol;Nelumbo nucifera stamens;$Fc{\varepsilon}RI$;GATA-1;KU812F cells

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