The Distribution Pattern of Eggs and Larval Survival Rate of Phyllocnistis citrella (Lepidoptera: Gracillaridae) on the Leaves of Shoots in a Citrus Orchard in Jeju

제주 감귤원에서 귤굴나방 알의 신초 잎 위치별 분포와 유충의 생존률

  • Hyun, Seung Young (Majors in Plant Resource Sciences & Environment, College of Applied Life Science, SARI, Jeju National University) ;
  • Kim, Su Bin (Majors in Plant Resource Sciences & Environment, College of Applied Life Science, SARI, Jeju National University) ;
  • Kim, Dong-Soon (Majors in Plant Resource Sciences & Environment, College of Applied Life Science, SARI, Jeju National University)
  • 현승용 (제주대학교 생명자원과학대학 식물환경전공, SARI) ;
  • 김수빈 (제주대학교 생명자원과학대학 식물환경전공, SARI) ;
  • 김동순 (제주대학교 생명자원과학대학 식물환경전공, SARI)
  • Received : 2019.01.17
  • Accepted : 2019.07.21
  • Published : 2019.09.01


The citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillaridae), is an important pest that decreases the productivity of citrus trees by attacking citrus leaves, and especially it causes a severe damage during the young growth stage. In this study, we investigated the distribution of eggs on the leaves of shoots according to the position and the survival rate of this pest in the field condition. The occurrence patterns of P. citrella eggs in citrus orchards were largely affected by the occurrence of shoots. Even if the adults were still being attracted in the pheromone traps, the eggs were not observed on leaves when the growth of shoots was stopped. The density of P. citrella eggs in the citrus shoots peaked at the fifth or sixth leaf from the terminal leaf, and over 90% of the eggs were found in the first to eighth leaves as a whole. In 2015 and 2016, the average survival rate of larvae surviving to pupa was 1.4%, and the rate of survival to adults was 0.2%, indicating that most of them died. Furthermore, we have discussed the management strategy of P. citrella considering the distribution of eggs and the larval survival rate in the citrus shoots.


Citrus leafminer;Seasonal abundance;Egg occurrence;Survival rate;Shoot growth


Supported by : RDA


  1. Achor, D.S., Browning, H., Albrigo, L.G., 1996. Anatomical and histochemical modifications in citrus leaves caused by larval feeding of citrus leafminer (Phyllocnistis citrella Staint), in: Hoy, M.A. (Ed.), Managing the Citrus Leafminer. Proceedings from an International Conference, Orlando, Florida, 23-25 April, 1996.
  2. Ba-Angood, S.A.S., 1977. A contribution to the biology and occurance of the citrus leafminer, Phyllocnlstls cltrella Stainton (Lepidoptera: Gracillariidae) in the Sudan. Z. Angew. Entomol. 83, 106-111.
  3. Badaway, A., 1967. The morphology and biology of P hyllocnistis citrella Staint., a citrus leaf miner in the Sudan. Bull. Soc. Entomol. Egypt 51, 95-103.
  4. Elekcioglu, Z., Uygun, N., 2004. The effect of temperature on development and fecundity of Phyllocnistis citrella Stainton (Lepidoptera: Gracillaridae). Turk. Entomol. Derg. 28, 83-93.
  5. Faeth, S., 1985. Host leaf selection by leafminers: interaction among three trophic levels. Ecology 66, 870-875.
  6. Foelkel, E., Redaelli, L.R., Jahnke, S.M., Losekann, P.B., 2009. Predation and parasitism of Cirrospilus neotropicus (Hymenoptera: Eulophidae) on Phyllocnistis citrella (Lepidoptera: Gracillariidae) in laboratory. Rev. Colomb. Entomol. 35, 156-162.
  7. Garcia-Mari, F., Vercher, R., Costa-Comelles, J., Marzal, C., Villalba, M., 2004. Establishment of Citrostichus phyllocnistoides (Hymenoptera: Eulophidae) as a biological control agent for the citrus leafminer Phyllocnistis citrella (Lepidoptera: Gracillariidae), in Spain. Biol. Con. 29, 215-226.
  8. Gyoutoku, Y., Murai, K., Miyata, T., Isoda, T., 1996. The resistance of the citrus leafminer, Phyllocnistis citrella Stainton, to insecticides and laboratory bioassay method. Jpn. J. Appl. Entomol. Zool. 40, 238-241.
  9. Heppner, J.B., 1993. Citrus leafminer, Phyllocnlstls cltrella, in Florida. Trop. Lep. 4, 49-64.
  10. Hill, G. F., 1918. History of citrus canker in the Northern Territory (with notes of its occurrence elsewhere). Bull. North. Territ. Aust. (Darwin) 18, 1-8.
  11. Hyun, S.Y., Elekcioglub, N.Z., Kim, S.B., Kwon, S.H., Kim, D.-S., 2017. Parameter estimation for temperature-driven immature development and oviposition models of Phyllocnistis citrella Stainton (Lepidoptera: Gracillaridae) in the laboratory. J. Asia-Pac. Entomol. 20, 802-808.
  12. Jeong, I.-H., Jeon, S.-W., Lee, S.-K., Park, B., Park, S.-K., Lee, S.-B., Choi, N. J., Lee, S.-W., Lee, S. H., Kwon, D. H., 2017. Insecticide cross-resistance and developmental characteristics on the two rice varieties, 'Chinnong' and 'Chuchung', of the imidacloprid-resistant brown planthopper. Korean J. Pestic. Sci. 21, 381-388.
  13. Knapp, J.L., Albrigo, L.G., Browning, H.W., 1995. Citrus leafminer, Phyllocnistis citrella Stainton: Current status in Florida. Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida.
  14. Legaspi, J.C., French, J.V., Schauff, M.E., Woolley, J.B., 1999. The citrus leafminer Phyllocnistis citrella (Lepidoptera: Gracillariidae) in South Texas: incidence and parasitism. Fla. Entomol. 82, 305-316.
  15. Lioni, A.S.R., Cividanes, F.J., 2004. Ecological life table of the citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae). Neotrop. Entomol. 33, 407-415.
  16. Ludwig, A.J., Reynolds, J.F., 1988. Statistical ecology: a primer on methods and computing, John Wiley & Sons, Inc, New York, 337 pp.
  17. Mari, F.G., Costa-Comelles, J., Vercher, R., Verdu, M.J., Aliaga, J.L., 1996. Population trends and native parasitoids of the citrus leafminer in Valencia (Spain), in: Hoy, M.A. (Ed.), Managing the Citrus Leafminer. Proceedings from an International Conference, Orlando, Florida, 23-25 April, 1996.
  18. Radke, K., Kandalkar, M., 1987. Bionomics of Phyllocnlstls cltrella Stainton (Lepidoptera: Gracillaridae). PKV Res. J. (Akola) 11, 91-92.
  19. Sahayaraj, K., Balasubramanian, R., 2016. Artificial rearing of reduviid predators for pest management. Springer, Singapore, 180 pp.
  20. SAS Institute, 1999. SAS System for Window, Release 8.02. SAS Institute, Cary, NC.
  21. Schauff, M.E., LaSalle, J., Wijesekara, G.A., 1998. The genera of chalcid parasitoids (Hymenoptera: Chalcidoidea) of citrus leafminer Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), J. Nat. Hist. 32, 1001-1056.
  22. Siu-King, L., Ren-Guang, Z., 1980. The morphology of the citrus leafminer (Phyllocnlstls cltrella Stainton) and its food plants in Kwangtung Province. J. South China, Agric. Coll. 1, 113-120.
  23. Sohi, G.S., Verma, G.S., 1966. Feeding habits of Phyllocnistis citrella Stainton in relation to the anatomical structure of the leaf. Indian J. Entomol. 55, 238.
  24. Song, J.H., Kang, S.-H., 2006. Responses of citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillariidae) for a sex pheromone component, (Z,Z)-7, 11-hexadecadienal on Jeju Island. Korean J. Appl. Entomol. 45, 161-167.
  25. Stainton, H.T., 1856. Descriptions of three species of Indian micro-Lepidoptera. Trans. Entomol. Soc. London (n.s.) 3, 301-304.
  26. Stansly, P., Rouse, R.E., 1993. Reduce winter flush, reduce citrus leafminer damage next spring. Citrus Ind. 7, 40-41.
  27. Vercher, R., Farias, A., Marzal, C., Soto, A., Tena, A., Garcia-Mari, F., 2008. Factors influencing adult female oviposition in the citrus leafminer Phyllocnistis citrella. Agric. For. Entomol. 10, 45-51.
  28. Wilson, C.G., 1991. Notes on Phyllocnlstls cltrella Stainton (Lepidoptera: Phyllocnististidae) attacking four citrus varieties in Darwin. Rev. Appl. Ent. (A) 80, 1345.
  29. Xiao, Y., Fadamiro, H.Y., 2010. Exclusion experiments reveal relative contributions of natural enemies to mortality of citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillariidae) in Alabama satsuma orchards. Biol. Control 54, 189-196.