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Protective Effect of Membrane-Free Stem Cell Extract against Oxidative Stress in LLC-PK1 Cells

무막줄기세포추출물의 LLC-PK1 세포에서의 산화적 스트레스 개선 효과

  • Kim, Min Jeong (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University) ;
  • Kim, Ji Hyun (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University) ;
  • Park, Hye Sook (T-STEM Co., Ltd.) ;
  • Kim, Young Sil (T-STEM Co., Ltd.) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University)
  • 김민정 (부산대학교 식품영양학과 및 김치연구소) ;
  • 김지현 (부산대학교 식품영양학과 및 김치연구소) ;
  • 박혜숙 ((주)티스템) ;
  • 김영실 ((주)티스템) ;
  • 조은주 (부산대학교 식품영양학과 및 김치연구소)
  • Received : 2019.05.07
  • Accepted : 2019.08.02
  • Published : 2019.08.31

Abstract

Oxidative stress in kidneys can precede the development of chronic renal injury. We investigated the antioxidative effect of membrane-free stem cell extract (MFSCE) from adipose tissue in LLC-$PK_1$ renal proximal tubule cells. Treatment of LLC-$PK_1$ cells with MFSCE showed the up-regulation of heme-oxygenase-1, thioredoxin reductase 1, and NADPH quinine oxidoreductase-1 protein expressions, which are proteins related with antioxidative activities. When oxidative stress was induced by 3-morpholinosydnonimine (SIN-1), cell viability was decreased, indicating that LLC-$PK_1$ cells were damaged by SIN-1. However, MFSCE significantly elevated cell viability from 58.84% to 64.43% at the concentration of $2.5{\mu}g/mL$ in oxidative stress-induced LLC-$PK_1$ cells. Furthermore, MFSCE ameliorated inflammation and apoptosis in SIN-1-treated LLC-$PK_1$ cells by modulating protein expressions. Inducible nitric oxide synthase and cyclooxygenase-2 protein expressions were down-regulated when LLC-$PK_1$ cells were treated with MFSCE. Apoptosis-related proteins, including B-cell lymphoma-2-associated X protein/B-cell lymphoma-2 ratio, cleaved caspase-3, and cleaved-poly (ADP-ribose) polymerase, were also down-regulated. It indicated that MFSCE protected apoptosis against oxidative stress in LLC-$PK_1$ cells. Taken together, these results suggested that MFSCE had a protective effect against SIN-1-induced oxidative stress in LLC-$PK_1$ cells. Therefore, MFSCE could be a promising therapeutic agent for oxidative stress-induced renal injury.

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