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Inhibitory Effects of Ethanol Extract of Rhodiola Sacra on Endoplasmic Reticulum Stress in Neuro-2A Cells

설치류 Neuro-2A 신경세포에서 홍경천 에탄올 추출물의 소포체 스트레스 억제효과

  • Jo, Nam-Eun (Alternative Medicine from Daejeon University) ;
  • Song, Young-soon (Graduate School of Oriental Culture Graduate School of Medicinal Crop Science)
  • 조남은 (대전대학교 대체의학과) ;
  • 송영순 (동방문화대학원대학교 약용작물학)
  • Received : 2019.06.03
  • Accepted : 2019.08.20
  • Published : 2019.08.28

Abstract

Growing evidence suggests that mediating apoptotic cell death of ER stress plays an important role in pathological development of neurodegenerative diseases including Alzheimer's disease. The ethanol extract of Rodiola sacra (ERS) investigates whether ER stress protects neuroinvasive neuro-2A cells from homocysteine (Hcy) cell death and ER stress. In neuronal cells, Hcy markedly decreased the viability of the cells and induced the death of Annexin V-positive cells as confirmed by MTT assay. The Hcy cell viability and apoptotic loss pretreated with ERS were attenuated, and Hcy showed stress in the expression of C / EBP homologous protein, 78-kDa glucose regulatory protein and the junction of X-box binding protein-1 (xbp1) mRNA. ESR decreased Hcy-induced mRNA binding, GRP78 and CHOP cells induced Hcy-induced ER stress and apoptosis, and Western blotting revealed expression of heme oxygenase-1 and HO-1 enzyme activity Inhibition is indicative of therapeutic value for neurodegenerative diseases such as decreased cell death by hemin.

Keywords

Tibet Hong Kyung Chun;defoliating stress;homocysteine;apoptosis;neuron;hemoxigenase-1

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