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Effects of protease supplementation on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs

  • Min, Yejin (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Yohan (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Younghwa (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Yongdae (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Doowan (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Joeun (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Hyunjung (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Song, Minho (Department of Animal Science and Biotechnology, Chungnam National University)
  • Received : 2019.07.09
  • Accepted : 2019.07.15
  • Published : 2019.07.31

Abstract

This experiment was conducted to evaluate the effects of dietary protease on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs. A total of 48 growing pigs (initial body weight, $34.8{\pm}0.62kg$) were randomly assigned to 2 dietary treatments (6 pigs/pen; 4 replicates/treatment). The treatments were a diet based on corn and soybean meal (CON) and CON supplemented with 0.01 % of protease (PRO). Pigs were fed respective dietary treatments with a 2-phase feeding program for 12 weeks. Pigs fed PRO had higher average daily gain (ADG; phase I, 866.38 vs. 821.75 g/d; overall, 910.96 vs. 866.30 g/d; p < 0.05) and gain to feed ratio (G:F; phase I, 0.345 vs. 0.363 g/g; p < 0.05) than those fed CON. However, there were no differences on blood constituents and carcass characteristics between CON and PRO of growing-finishing pigs. In conclusion, dietary protease supplementation in the typical diet for growing-finishing pigs improved growth rate.

Acknowledgement

Grant : Cooperative Research Program for Agriculture Science and Technology Development

Supported by : Rural Development Administration

References

  1. Huisman J, Jansman AJM. Dietary effects and some analytical aspects of antinutritional factors in peas (Pisum sativum), common beans (Phaseolus vulgaris) and soyabeans (Glycine max) in monogastric farm animals. Nutr Abstr Rev. 1991;61:901-21.
  2. Tamminga S, Schulze H, Van Bruchem J, Huisman J. The nutritional significance of endogenous n-losses along the gastrointestinal tract of farm animals. Arch fur Tierernahr. 1995;48:9-22. https://doi.org/10.1080/17450399509381824
  3. Raemaekers RH. Crop production in tropical Africa. Brussels, Belgium: DGIC; 2001. p.809-28.
  4. Nsoh A. Growth performance, blood profile and carcass characteristics of growing pigs fed diets containing varying levels of soybean milk residue (SBMR). http://hdl.handle.net/123456789/5322. Accessed 10 Aug 2013.
  5. Caine WR, Tamminga S, Verstegen MW, Sauer WC, Schulze H. Endogenous recoveries and true ileal digestibilities of amino acids in newly weaned pigs fed diets with protease-treated soybean meal. J Anim Sci. 1997;75:2970-9. https://doi.org/10.2527/1997.75112970x
  6. Mackie RI, Stroot PG, Varel VH. Biochemical identification and biological origin of key odor components in livestock waste. J Anim Sci. 1998;76:1331-42. https://doi.org/10.2527/1998.7651331x
  7. Sutton AL, Kephart KB, Verstegen MWA, Canh TT, Hobbs PJ. Potential for reduction of odorous compounds in swine manure through diet modification. J Anim Sci. 1999;77:430-9. https://doi.org/10.2527/1999.772430x
  8. Adeola O, Cowieson AJ. Opportunities and challenges in using exogenous enzymes to improve non-ruminant animal production. J Anim Sci. 2011;89:3189-218. https://doi.org/10.2527/jas.2010-3715
  9. Yin YL, Baidoo SK, Jin LZ, Liu YG, Schulze H, Simmins PH. The effect of different carbohydrase and protease supplementation on apparent (ileal and overall) digestibility of nutrients of five hulless barley varieties in young pigs. Livest Prod Sci. 2001;71:109-20. https://doi.org/10.1016/S0301-6226(01)00215-9
  10. Omogbenigun FO, Nyachoti CM, Slominski BA. Dietary supplementation with multienzyme preparations improves nutrient utilization and growth performance in weaned pigs. J Anim Sci. 2004;82:1053-61. https://doi.org/10.2527/2004.8241053x
  11. Yin YL, Deng ZY, Huang HL, Li TJ, Zhong HY. The effect of arabinoxylanase and protease supplementation on nutritional value of diets containing wheat bran or rice bran in growing pig. J Anim Feed Sci, 2004;13:445-61.
  12. Ji F, Casper DP, Brown PK, Spangler DA, Haydon KD, Pettigrew JE. Effects of dietary supplementation of an enzyme blend on the ileal and fecal digestibility of nutrients in growing pigs. J Anim Sci. 2008;86:1533-43. https://doi.org/10.2527/jas.2007-0262
  13. Jo JK, Ingale SL, Kim JS, Kim YW, Kim KH, Lohakare JD, et al. Effects of exogenous enzyme supplementation to corn- and soybean meal-based or complex diets on growth performance, nutrient digestibility, and blood metabolites in growing pigs. J Anim Sci. 2012;90:3041-8. https://doi.org/10.2527/jas.2010-3430
  14. Upadhaya SD, Yun HM, Kim IH. Influence of low or high density corn and soybean meal-based diets and protease supplementation on growth performance, apparent digestibility, blood characteristics and noxious gas emission of finishing pigs. Anim Feed Sci Technol. 2016;216:281-7. https://doi.org/10.1016/j.anifeedsci.2016.04.003
  15. Zuo J, Ling B, Long L, Li T, Lahaye L, Yang C, et al. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. Anim Nutr. 2015;1:276-82. https://doi.org/10.1016/j.aninu.2015.10.003
  16. Choe J, Kim KS, Kim HB, Park S, Kim J, Kim S, et al. Effect of protease on growth performance and carcass characteristics of growing-finishing pigs. S Afr J Anim Sci. 2017;47:697-703. https://doi.org/10.4314/sajas.v47i5.13
  17. Kong C, Park CS, Kim BG. Effects of an enzyme complex on in vitro dry matter digestibility of feed ingredients for pigs. SpringerPlus. 2015;4:261. https://doi.org/10.1186/s40064-015-1060-1
  18. O’Shea CJ, Mc Alpine PO, Solan P, Curran T, Varley PF, Walsh AM, et al. The effect of protease and xylanase enzymes on growth performance, nutrient digestibility, and manure odour in grower-finisher pigs. Anim Feed Sci Technol. 2014;189:88-97. https://doi.org/10.1016/j.anifeedsci.2013.11.012
  19. Mc Alpine PO, O’shea CJ, Varley PF, O’Doherty JV. The effect of protease and xylanase enzymes on growth performance and nutrient digestibility in finisher pigs. J Anim Sci. 2012;90:375-7. https://doi.org/10.2527/jas.53979
  20. Thacker PA. Effect of xylanase and protease on the performance of growing-finishing pigs fed corn-based diets. J Appl Anim Res. 2015;28:17-23.
  21. Guggenbuhl P, Wach Y, Wilson JW. Effects of dietary supplementation with a protease on the apparent ileal digestibility of the weaned piglet. J Anim Sci. 2012;90:152-4.
  22. O’Doherty JV, Forde S. The effect of protease and ${\alpha}$-galactosidase supplementation on the nutritive value of peas for growing and finishing pigs. Irish J Agri Food Res. 1995;38:217-26.
  23. Tactacan GB, Cho SY, Cho JH, Kim IH. Performance responses, nutrient digestibility, blood characteristics, and measures of gastrointestinal health in weanling pigs fed protease enzyme. Asian-Australas J Anim Sci. 2016;29:998-1003. https://doi.org/10.5713/ajas.15.0886
  24. Cowieson AJ, Roos FF. Bioefficacy of a mono-component protease in the diets of pigs and poultry: a meta-analysis of effect on ileal amino acid digestibility. J Appl Anim Nutr. 2013;2:e13. https://doi.org/10.1017/jan.2014.5
  25. Lichtman M, Beutler E, Kipps T, Seligsohn U, Kaushansky K, Prchal J. Williams hematology. 8th ed. New York, NY: Mc-Graw-Hill; 2010. p.823-39.
  26. Islam MA, Grobe-Brinkhaus C, Proll MJ, Uddin MJ, Rony SA, Tesfaye D, et al. Deciphering transcriptome profiles of peripheral blood mononuclear cells in response to PRRSV vaccination in pigs. BMC genomics. 2016;17:641. https://doi.org/10.1186/s12864-016-2849-1
  27. Dubreuil P, Couture Y, Farmer C, Petitclerc D. Hematological and biochemical changes following an acute stress in control and somatostatin-immunized pigs. Can J Anim Sci. 1993;73:241-52. https://doi.org/10.4141/cjas93-026
  28. Choe J, Kim KS, Kim HB, Park S, Kim J, Kim S, et al. Effects of protease on growth performance and carcass characteristics of growing-finishing pigs. S Afr J Anim Sci. 2017;47:697-703. https://doi.org/10.4314/sajas.v47i5.13
  29. Stephenson EW, DeRouchey JM, Escobar J, Woodworth J, Tokach MD, Goodband RD, et al. Effects of a novel protease enzyme (CIBENZA DP100) on finishing pig growth performance and carcass characteristics. Kans Agric Exp Stn Res Rep. 2014;69-76.
  30. Wang D, Piao XS, Zeng ZK, Lu T, Zhang Q, Li PF, et al. Effects of keratinase on performance, nutrient utilization, intestinal morphology, intestinal ecology and inflammatory response of weaned piglets fed diets with different levels of crude protein. Asian-Australas J Anim Sci. 2011;24:1718-28. https://doi.org/10.5713/ajas.2011.11132