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Protective Effects of Membrane-Free Stem Cell Extract from H2O2-Induced Inflammation Responses in Human Periodontal Ligament Fibroblasts

무막줄기세포추출물의 H2O2에 의해 유도된 치주 세포의 염증 반응 보호 효과

  • He, Mei Tong (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Ji Hyun (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Young Sil (T-STEM Co., Ltd.) ;
  • Park, Hye Sook (T-STEM Co., Ltd.) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • Received : 2019.02.26
  • Accepted : 2019.06.07
  • Published : 2019.06.30

Abstract

Periodontal inflammation, a major kind of periodontal diseases, is characterized to bleed, pain, and teeth loss, and it is resulted from oxidative stress. Membrane-free stem cell extract could avoid the immunogencity rejection by removal of cell membrane. In the present study, we investigated the protective effect of membrane-free stem cell extract from oxidative stress-induced periodontal inflammation in human periodontal ligament fibroblasts (HPLF). In the cell viability measurement, membrane-free stem cell extract showed significant increase of cell viability, compared with the $H_2O_2$-treated control group. To further investigation of molecular mechanisms, we measured inflammation and apoptosis related protein expressions. Membrane-free stem cell extract attenuated inflammation-related protein expressions such as nuclear factor kappa light chain enhancer of activated B cells, inducible nitric oxide synthase, and interleukin-6. In addition, the treatment of membrane-free stem cell extract decreased apoptotic protein expressions such as cleaved caspase-9, -3, poly (ADP-ribose) polymerase, and B-cell lymphoma 2 (Bcl-2)-associated X protein/Bcl-2 ratio in the $H_2O_2$-treated HPLF cells. In conclusion, membrane-free stem cell extract exhibited anti-oxidative stress effects by regulation of inflammation and apoptosis in HPLF, suggesting that it could be used as the treatment agents for periodontal inflammatory disease.

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Fig. 1. Effect of membrane-free stem cell extract on H2O2-treated HPLF cells. Values are presented as mean ± SD. The different letters (a-c) indicate significant differences (P < 0.05) by Duncan’s multiple range test.

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Fig. 2. Effect of membrane-free stem cell extract on inflammation-related protein expression in H2O2-treated HPLF cells. Values are presented as mean ± SD. The different letters (a-e) indicate significant differences (P < 0.05) by Duncan’s multiple range test.

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Fig. 3. Effect of membrane-free stem cell extract on apoptosis-related protein expression such as caspase-9, -3, and PARP in H2O2-treated HPLF cells. Values are presented as mean ± SD. The different letters (a-d) indicate significant differences (P < 0.05) by Duncan’s multiple range test.

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Fig. 4. Effect of membrane-free stem cell extract on apoptosis-related protein expression such as Bax and Bcl-2 in H2O2-treated HPLF cells. Values are presented as mean ± SD. The different letters (a-d) indicate significant differences (P < 0.05) by Duncan’s multiple range test.

Table 1. Cytotoxicity of membrane-free stem cell extract on HPLF cells

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