DOI QR코드

DOI QR Code

Proximate Analysis, Fatty Acid Composition of Lycopus lucidus Turcz. and Its Cytotoxic Effect in Cancer Cell Lines

택란의 일반성분, 지방산 조성 및 세포독성 효과

Na, Eun;Lee, Jung-Woo;Lim, Sun-Young
나은;이정우;임선영

  • Received : 2019.04.04
  • Accepted : 2019.05.14
  • Published : 2019.06.30

Abstract

In this paper, we investigate to determine quality characteristics, fatty acid composition and cytotoxic effect of extracts and fractions from whole Lycopus lucidus Turcz. roots. Additionally, we evaluated cytotoxic activity against the growth of human fibrosarcoma cells (HT-1080) and human gastric adenocarcinoma (AGS), human colon cancer cell (HT-29) lines using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acetone+methylene chloride (A+M) and methanol (MeOH) extracts from L. lucidus Turcz. were obtained through solvent extraction. Then we further fractionated both extracts with n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water. In fatty acid composition, L. lucidus Turcz. contained 33.2% of 18:1n-9 and 1.81% of 18:3n-3, respectively. The incorporation of treatment with A+M and MeOH extracts and n-hexane, 85% aq. MeOH, n-butanol (n-BuOH) and water fractions dose-dependently increased cytotoxicity against the growth of HT-1080 and AGS, HT-29 cancer cells (p<0.05). The A+M extract had a higher inhibitory effect on the growth of all cancer cells in comparison to MeOH extract. Among the fractions, the 85% aq. MeOH and n-hexane fractions showed a higher inhibitory effect after proliferating the three cancer cells. These results suggest that the 85% aq. MeOH and n-hexane fractions have a potential to inhibit the growth of human cancer cell lines.

Keywords

Lycopus lucidus Turcz;fatty acid composition;human cancer cell;cytotoxicity

References

  1. Yang X, Zhao Y, He N, Croft KD. 2010. Isolation, characterization, and immunological effects of ${\alpha}$-galacto-oligosaccharides from a new source, the Herb Lycopus lucidus Tucz. J Agric Food Chem 58:8253-8258 https://doi.org/10.1021/jf101217f
  2. Yu JQ, Lei JC, Zhang XQ, Yu HD, Tian DZ, Liao ZX, Zou GL. 2011. Anticancer, antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz. var. hirtus Regel. Food Chem 126:1593-1598 https://doi.org/10.1016/j.foodchem.2010.12.027
  3. AOAC. 1996. The Association Official Methods of Analysis. 16th ed. pp.210-219. Association of Official Analytical Chemists
  4. Carrillo C, Cavia M, Alonso-Torre SR. 2012. Antitumor effect of oleic acid: Mechanisms of action: A review. Nutr Hosp 27:1860-1865
  5. Choi IS, Cha EJ, Lee YR, Kim JK. 2012. Antioxidant and anticancer activities of Yak-Sun tea prepared by oriental medicinal herbs. Korean J Food Nutr 25:447-453 https://doi.org/10.9799/ksfan.2012.25.3.447
  6. Dai J, Shen J, Pan W, Sehn S, Das UN. 2013. Effects of polyunsaturated fatty acids on the growth of gastric cancer cells in vitro. Lipids Health Dis 12:71 https://doi.org/10.1186/1476-511X-12-71
  7. Denizot F, Lang R. 1986. Rapid colorimetric assay for cell growth and survival. modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89:271-277 https://doi.org/10.1016/0022-1759(86)90368-6
  8. Dommels YE, Alink GM, Linssen JP, Van Ommen B. 2002. Effects of n-6 and n-3 polyunsaturated fatty acids on gap junctional intercellular communication during spontaneous differentiation of the human colon adenocarcinoma cell line Caco-2. Nutr Cancer 42:125-130 https://doi.org/10.1207/S15327914NC421_17
  9. Folch J, Lee M, Sloane Stanley GH. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497-509
  10. Juan ME, Wenzel U, Ruiz-Gutierrez V, Daniel H, Planas JM. 2006. Olive fruit extracts inhibit proliferation and induce apoptosis in HT-29 human colon cancer cells. J Nutr 136:2553-2557 https://doi.org/10.1093/jn/136.10.2553
  11. Kim CM, Shin MK, Ahn DK, Lee KS. 1997. The Encyclopedia of Oriental Herbal Medicine. 3rd ed. pp.4278, 4398-4399. Jungdam Books
  12. Kim DY, Ghil SH. 2009. Effect of Lycopus lucidus Trucz on cell growth of human breast cancer cells, MCF-7. Biomed Sci Lett 15:147-152
  13. Kim KY, Oh TW, Ma JY, Park KI. 2018. Ethanol extract of Lycopus lucidus Turcz. ex Benth inhibits metastasis by downregulation of Runx-2 in mouse colon cancer cells. J Evidence-Based Complementary Altern Med 2018, p.9513290
  14. Kim MC, Lee HJ, Lim B, Ha KT, Kim SY, So I, Kim BJ. 2014. Quercetin induces apoptosis by inhibiting MAPKs and TRPM7 channels in AGS cells. Int J Mol Med 33:1657-1663 https://doi.org/10.3892/ijmm.2014.1704
  15. Kim YK, Son HK, Lee JJ. 2017. Nutritional components and antioxidant activities of various Stachys sieboldii Miq. parts. Korean J Community Living Sci 28:203-215 https://doi.org/10.7856/kjcls.2017.28.2.203
  16. Lee JE, Jin SY, Han YS. 2014. Antioxidant activities and quality characteristics of tofu supplemented with Chinese artichoke powder. Korean J Food Nutr 27:10-21 https://doi.org/10.9799/ksfan.2014.27.1.010
  17. Lee JW, Lim SY. 2018. Comparison of flavonoid content and antioxidant effect of extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz. J Life Sci 28:841-848
  18. Lee SW, Jung TH, Shin YW 2013. A comparative study of memory improving effects of Stachys rhizome and Lycopi rhizome on scopolamine-induced amnesia in mice. Korean J Herbol 28:69-77
  19. Lee YJ, Kang DG, Kim JS, Lee HS. 2008. Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells. Vascul Pharmacol 48:38-46 https://doi.org/10.1016/j.vph.2007.11.004
  20. Lin CR, Zuo SY, Xiong W, Chen GY. 2012. Antioxidation effects of Lycopus lucidus polysaccharides on aged mice induced by D-galactose. Med Plant 3:53-55
  21. Lu YH, Huang JH, Li YC, Ma TT, Sang P, Wang WJ, Gao CY. 2015. Variation in nutritional compositions, antioxidant activity and microstructure of Lycopus lucidus Turcz. root at different harvest times. Food Chem 183:91-100 https://doi.org/10.1016/j.foodchem.2015.03.033
  22. Morrison WR, Smith LM. 1964. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoridemethanol. J Lipid Res 5:600-608
  23. Na HG, Jang EH, Nam DH, Kim MA, Kim MJ, Sohn EH, Kim HD, Jang KH. 2018. Physicochemical properties and isoflavone content of Chaga Cheonggukjang with Lactobacillus acidophilus KCTC 3925 starter. Korean J Food Preserv 25:221-228 https://doi.org/10.11002/kjfp.2018.25.2.221
  24. Park HJ, Jin S, Oh YN, Yun SG, Lee JY, Kwon HJ, Kim BW. 2013. Induction of G1 arrest by methanol extract of Lycopus lucidus in human lung adenocarcinoma A549 cells. J Life Sci 23:1109-1117 https://doi.org/10.5352/JLS.2013.23.9.1109
  25. Ryu BH. 1991. Eating habits preventing against cancer. Korean J Food Nutr 4:213-229
  26. Salem N, Litman B, Kim HY, Gawrisch K. 2001. Mechanisms of action of docosahexaenoic acid in the nervous system. Lipids 36:945-959 https://doi.org/10.1007/s11745-001-0805-6
  27. Salem N, Reyer M, Karanian J. 1996. Losses of arachidonic acid in rat liver after alcohol inhalation. Lipids 31:S153-156 https://doi.org/10.1007/BF02637068
  28. Song YJ, Chang JP, Yoo JH. 2016. Antioxidant activities of water extracts from different parts of Lycopus lucidus Turcz. ex Benth. Korea J Herbaol 31:21-28
  29. Theodoratou E, Kyle J, Cetnarskyj R, Farrington SM, Tenesa A, Barnetson R, Porteous M, Dunlop M, Campbell H. 2007. Dietary flavonoids and the risk of colorectal cancer. Cancer Epidemiol Biomarkers Prev 16:684-693 https://doi.org/10.1158/1055-9965.EPI-06-0785
  30. Xiong W, Jiao Y, Luo YH, Zhang CX, Tan DY, Zuo SY. 2012. Effects of polysaccharide from Lycopus lucidus Turcz. on proliferation of tumor cells in vitro and in vivo. China J Bioprocess Eng 10:64-69
  31. Xu M, Chen YM, Huang J, Fang YJ, Huang WQ, Yan B, Lu MS, Pan ZZ, Zhang GX. 2016. Flavonoid intake from vegetables and fruits is inversely associated with colorectal cancer risk: A case-control study in China. Br J Nutr 116:1275-1287 https://doi.org/10.1017/S0007114516003196
  32. Yang MO. 2017. Antioxidant properties of hot water extract of Lycopus lucidus Trucz. tubers. Korean J Community Living Sci 28:103-113 https://doi.org/10.7856/kjcls.2017.28.1.103

Acknowledgement

Supported by : 한국연구재단