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Complete genome sequencing of Pseudomonas fluorescens NBC275, a biocontrol agent against fungal pathogens of plants and insects

식물 및 곤충의 곰팡이 병원균에 항균력을 가진 Pseudomonas fluorescens NBC275 균주의 유전체 염기서열

Dutta, Swarnalee;Yu, Sang-Mi;Nagendran, Rajalingam;Jeong, Sang Chul;Lee, Yong Hoon
더타 스와나리;유상미;나젠드란 라자링감;정상철;이용훈

  • Received : 2019.02.13
  • Accepted : 2019.03.01
  • Published : 2019.06.30

Abstract

Pseudomonas fluorescens NBC275 (Pf275) isolated from soil sample collected at riverside of Nakdonggang showed antagonistic activity against fungal pathogens of plants and insects. Here we present complete genome sequence of Pf275. The genome comprises of 6,610,362 bp with GC content of 60.9%, which includes 5,869 predicted protein-coding genes, 16 rRNAs, and 65 tRNAs. Genome analysis revealed gene clusters encoding antimicrobial secondary metabolites such as pyoverdine, 2, 4-diacetylphloroglucinol, and phenazine, which are known to play essential roles in biocontrol of diseases.

Keywords

Pseudomonas fluorescens;antagonism;biocontrol;genome sequence;secondary metabolite

References

  1. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, et al. 2013. Nonhybrid, finished microbial genome assemblies from longread SMRT sequencing data. Nat. Methods 10, 563-569. https://doi.org/10.1038/nmeth.2474
  2. Couillerot O, Combes-Meynet E, Pothier J, Bellvert F, Challita E, Poirier M, Rohr R, Comte G, Moenne-Loccoz Y, and Prigent-Combaret C. 2011. The role of the antimicrobial compound 2,4-diacetylphloroglucinol in the impact of biocontrol Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators. Microbiology 157, 1694-1705. https://doi.org/10.1099/mic.0.043943-0
  3. Couillerot O, Prigent-Combaret C, Caballero-Mellado J, and Moenne-Loccoz Y. 2009. Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens. Lett. Appl. Microbiol. 48, 505-512.
  4. Ghirardi S, Dessaint F, Mazurier S, Corberand T, Raaijmakers JM, Meyer JM, Dessaux Y, and Lemanceau P. 2012. Identification of traits shared by rhizosphere-competent strains of fluorescent pseudomonads. Microb. Ecol. 64, 725-737. https://doi.org/10.1007/s00248-012-0065-3
  5. Gliese N, Khodaverdi V, and Gorisch H. 2010. The PQQ biosynthetic operons and their transcriptional regulation in Pseudomonas aeruginosa. Arch. Microbiol. 192, 1-14. https://doi.org/10.1007/s00203-009-0523-6
  6. Haas D and Keel C. 2003. Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annu. Rev. Phytopathol. 41, 117-153. https://doi.org/10.1146/annurev.phyto.41.052002.095656
  7. Hernandez-Salmeron JE, Moreno-Hagelsieb G, and Santoyo G. 2017. Genome comparison of Pseudomonas fluorescens UM270 with related fluorescent strains unveils genes involved in rhizosphere competence and colonization. J. Genomics 5, 91-98. https://doi.org/10.7150/jgen.21588
  8. Redondo-Nieto M, Barret M, Morrissey J, Germaine K, Martinez-Granero F, Barahona E, Navazo A, Sanchez-Contreras M, Moynihan JA, Muriel C, et al. 2013. Genome sequence reveals that Pseudomonas fluorescens F113 possesses a large and diverse array of systems for rhizosphere function and host interaction. BMC Genomics 14, 54. https://doi.org/10.1186/1471-2164-14-54
  9. Silby MW, Cerdeno-Tarraga AM, Vernikos GS, Giddens SR, Jackson RW, Preston GM, Zhang XX, Moon CD, Gehrig SM, Godfrey SA, et al. 2009. Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens. Genome Biol. 10, R51. https://doi.org/10.1186/gb-2009-10-5-r51
  10. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Pruitt KD, Borodovsky M, and Ostell J. 2016. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res. 44, 6614-6624. https://doi.org/10.1093/nar/gkw569

Acknowledgement

Supported by : Nakdonggang National Institute of Biological Resources, National Research Foundation of Korea (NRF)