Effects of SPAC1B3.08, ortholog of Thp1/PCID2, on mRNA export in fission yeast

분열효모에서 Thp1/PCID2의 이종상동체인 SPAC1B3.08이 mRNA 방출에 미치는 영향

Park, Jin Hee;Yoon, Jin Ho

  • Received : 2019.05.27
  • Accepted : 2019.06.05
  • Published : 2019.06.30


Thp1/PCID2 is a subunit of the evolutionally conserved TREX-2 complex, which is required for transcription-coupled mRNA export from the nucleus to the cytoplasm. In fission yeast, Schizosaccharomyces pombe, there are two orthologs of the Thp1/PCID2 protein. In addition to pci2 (SPBC1105.07c) gene, SPAC1B3.08 gene encodes a PCI domain-containing protein that is predicted as a component of TREX-2 complex. Overexpression of SPAC1B3.08 cause slight defects of both growth and mRNA export. Yeast two-hybrid and co-immunoprecipitation analysis exhibits that the SPAC1B3.08 protein interacted with Sac3 and Dss1, which are another components of TREX-2 complex. These observations support the possibility that the S. pombe SPAC1B3.08 protein, as a component of TREX-2 complex, is involved in mRNA export.


S. pombe;mRNA export;orthologs of Thp1/PCID2;TREX-2 complex


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