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Changes in the Antioxidant Potential of Persimmon Peel Extracts Prepared by Different Extraction Methods

고종시 감껍질의 추출조건에 따른 항산화 활성

  • 정명진 (농업회사법인제이비에프(주)) ;
  • 진순우 (충남대학교 약학대학) ;
  • 화성용 (진주 바이오푸드) ;
  • 방희옥 (농업회사법인제이비에프(주)) ;
  • 한동문 (농업회사법인제이비에프(주)) ;
  • 전지영 (농업회사법인제이비에프(주)) ;
  • 화세경 (농업회사법인제이비에프(주))
  • Received : 2019.04.08
  • Accepted : 2019.05.26
  • Published : 2019.06.30

Abstract

Background: Astringent persimmon (Diospyros kaki Thunb. cv. Kojongsi) peels are by-products of dried persimmons. This study aimed to evaluate the antioxidant activities of Kojongsi persimmon peel (KPP) extracts prepared by 15 different extraction methods: 5 heating durations (0.5 - 2.5 h) at 3 heating temperatures (50, 70, and $90^{\circ}C$). Methods and Results: An increase in heating temperature increased the antioxidant effect of KPP extracts. Those prepared by heating at 1 h had the highest total phenol content, regardless of the heating temperature. In addition, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and cell-protective effects against $H_2O_2-induced$ oxidative stress were dependent on the total phenol contents of the extract. However, the KPP-induced increased in catalase expression was dependent on heating temperature and duration. Conclusions: These results suggest that extraction by heating at $90^{\circ}C$ for 1 h may enhance KPP's antioxidant effects, which mainly involve non-enzymatic antioxidant systems.

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Fig. 1. Effects of Kojongsi persimmon peel extracts prepared by treatment at different extracting conditions on DPPH radical-scavenging activity.

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Fig. 2. Protective effects of Kojongsi persimmon peel extracts prepared by treatment at different extracting conditions against oxidative stress (H2O2)-induced cellular damage in endothelial EA.hy926 cells.

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Fig. 3. Effects of Kojongsi persimmon peel extracts prepared by treatment at different extracting conditions on antioxidant enzyme expression in endothelial cells.

Table 1. Total phenol contents of Kojongsi persimmon peel extracts prepared by treatment at different extracting conditions.

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Acknowledgement

Supported by : Natural Materials by Gyeongnam Technopark

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