Establishment and validation of an analytical method for quality control of health functional foods derived from Agastache rugosa

  • Received : 2019.04.04
  • Accepted : 2019.05.31
  • Published : 2019.06.25


Agastache rugosa, known as Korean mint, is a medicinal plant with many beneficial health effects. In this study, a simple and reliable HPLC-UV method was proposed for the quantification of rosmarinic acid (RA) in the aqueous extracts of A. rugosa. RA was selected as a quantification marker due to its easiness in procurement and analysis. The developed method involved chromatographic separation on a $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}m$) at room temperature. The mobile phase consisted of water and acetonitrile both containing 2 % acetic acid and was run at a flow rate of $1mL\;min^{-1}$. The method was validated for specificity, linearity, precision, and accuracy. It was specific to RA and linear in the range of $50-300{\mu}g\;mL^{-1}$ ($r^2=0.9994$). Intra-day, inter-day, and inter-analyst precisions were ${\leq}0.91%\;RSD$, ${\leq}1.40%\;RSD$, and 1.94 % RSD, respectively. Accuracy was 93.3-95.9 % (${\leq}1.21%\;RSD$). The method could be applied to three batches of bulk samples and three batches of lab scale samples, which were found to be $0.64({\pm}0.04)mg\;g^{-1}$ and $0.48({\pm}0.02)mg\;g^{-1}$ for the dried raw materials of A. rugosa. The results show that the proposed method can be used as a readily applicable method for QC of health functional foods containing the aqueous extracts of A. rugosa.


Agastache rugosa;rosmarinic acid;health functional food;quality control

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Fig. 1. UHPLC-QTOF/MS spectra of (a) RA standard in methanol (100 μg mL−1), (b) non-spiked AE sample, and (c) AE sample spiked with RA at 100 μg mL−1.

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Fig. 2. HPLC-UV chromatograms of (a) methanol, (b) RA standard in methanol (100 μg mL−1), (c) non-spiked AE sample, and (d) AE sample spiked with RA at 100 μg mL−1. Peak ID: 1, RA.

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Fig. 3. HPLC-PDA spectra of (a) RA standard in methanol (100 μg mL−1), (b) non-spiked AE sample, (c) AE sample spiked with RA standard at 100 μg mL−1.

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Fig. 4. An HPLC chromatogram of the AE sample prepared in lab scale. Peak ID: 1, RA.

Table 1. Linearity of the established method (n = 3)

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Table 2. Intra- and inter-day precisions of the proposed method

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Table 3. Accuracy of the proposed method (n = 5)

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Supported by : Korea Small and Medium Business Administration (SMBA)


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