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Association of polymorphisms in bone morphogenetic protein receptor-1B gene exon-9 with litter size in Dorset, Mongolian, and Small Tail Han ewes

  • Jia, Jianlei (Key of laboratory of Plateau Ecology and Agriculture, Qinghai University) ;
  • Chen, Qian (Department of Animal Science, College of agriculture and Animal Husbandry, Qinghai University) ;
  • Gui, Linsheng (Key of laboratory of Plateau Ecology and Agriculture, Qinghai University) ;
  • Jin, Jipeng (Department of Animal Science, College of Animal Science and Technology, Gansu Agricultural University) ;
  • Li, Yongyuan (Animal Husbandry and Veterinary Station) ;
  • Ru, Qiaohong (Animal Husbandry and Veterinary Station) ;
  • Hou, Shengzhen (Department of Animal Science, College of agriculture and Animal Husbandry, Qinghai University)
  • Received : 2018.07.20
  • Accepted : 2019.01.16
  • Published : 2019.07.01

Abstract

Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The ${\chi}^2$ independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The ${\chi}^2$ independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.

Keywords

Sheep;Bone Morphogenetic Protein Receptor-1B (BMPR-1B) Gene;Polymorphisms;Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP);Litter Size

Acknowledgement

Supported by : National Natural Science Foundation of China, National Natural Science Foundation of Qinghai Province

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