The impact of dietary linseed oil and pomegranate peel extract on broiler growth, carcass traits, serum lipid profile, and meat fatty acid, phenol, and flavonoid contents

  • Kishawy, Asmaa TY (Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University) ;
  • Amer, Shimaa A (Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University) ;
  • El-Hack, Mohamed E Abd (Department of Poultry, Faculty of Agriculture, Zagazig University) ;
  • Saadeldin, Islam M (Department of Animal Production, College of Food and Agriculture Sciences, King Saud University) ;
  • Swelum, Ayman A (Department of Animal Production, College of Food and Agriculture Sciences, King Saud University)
  • Received : 2018.07.11
  • Accepted : 2018.12.31
  • Published : 2019.08.01


Objective: The current study aimed to replace soybean oil in broiler diets with linseed oil, which is rich in omega-3 fatty acid supplemented with pomegranate peel extract (PPE) and measured its effect on broiler performance, carcass traits, lipid profile, as well as fatty acids composition, phenols and flavonoids content of broiler muscles and immunity of broiler chicks. Methods: A total of 300 1-day-old Cobb chicks were randomly allotted into six experimental groups, T1 fed on basal diet with soybean oil without any additives, T2 fed on basal diet with soybean oil with addition of 0.5 g/kg diet PPE, T3 fed on fed on basal diet with soybean oil with addition of 1 g/kg diet PPE, T4 fed on basal diet with linseed oil without any additives, T5 fed on basal diet with linseed oil with addition of 0.5 g/kg diet PPE and T6 fed on basal diet with linseed oil with addition of 1 g/kg diet PPE. The PPE supplementation with 0.05% improved final body weight with either soybean oil ration or linseed oil ration. Results: The PPE improved carcass dressing percentage in comparison with the control groups. Body fat levels decreased with increasing PPE levels, especially with a linseed oil diet. Replacing soybean oil with linseed oil decreased the total cholesterol and triacylglycerol levels in broiler serum. The PPE supplementation decreased serum total cholesterol levels and increased high-density lipoprotein cholesterol levels. The content of the breast muscle alpha linolenic acid improved after replacement of soybean oil with linseed oil in broiler diets. PPE supplementation increased the phenol and flavonoid content in broiler meat and increased lysozyme activity. Conclusion: Replacing soybean oil with linseed oil in broiler diets with the addition of PPE enriched muscle meat with omega-3 fatty acids and antioxidants and improved broiler immunity and their serum lipid profile.


Supported by : King Saud University


  1. Biswas A, Ahmed M, Bharti VK, Singh SB. Effect of antioxidants on physio-biochemical and hematological parameters in broiler chicken at high altitude. Asian-Austrlras J Anim Sci 2011;24:246-9.
  2. Lawrie R, Ledward D. Lawrie's meat science. Seventh English, edition ed. Cambridge England: Woodhead Publishing Limited; 2006.
  3. Harris WS. Fish oils and plasma lipid and lipoprotein metabolism in humans: a critical review. J Lipid Res 1989;30:785-807.
  4. Freese R, Mutanen M, Valsta L, Salminen I. Comparison of the effects of two diets rich in monounsaturated fatty acids differing in their linoleic/alpha-linolenic acid ratio on platelet aggregation. Thromb Haemost 1994;71:73-7.
  5. Serhan CN, Hong S, Gronert K, et al. Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals. J Exp Med 2002;196:1025-37.
  6. Erkkila AT, Lichtenstein AH, Mozaffarian D, Herrington DM. Fish intake is associated with a reduced progression of coronary artery atherosclerosis in postmenopausal women with coronary artery disease. Am J Clinic Nutr 2004;80:626-32.
  7. El-Beltagi H, Salama Z, El-Hariri D. Evaluation of fatty acids profile and the content of some secondary metabolites in seeds of different flax cultivars (Linum usitatissimum L.). Gen Appl Plant Physiol 2007;33:187-202.
  8. Morrisey P, Brandon S, Buckley D, Sheey P, Frigg M. Tissue content of ${\alpha}$-tocopheryl acetate supplement for various periods pre-slaughter. Br Poult Sci 1997;38:84-8.
  9. Gray J, Gomaa E, Buckley D. Oxidative quality and shelf life of meats. Meat Sci 1996;43:111-23.
  10. Valenzuela A, Nieto S. Synthetic and natural antioxidants: food quality protectors. Grasas y aceites 1996;47:186-96.
  11. Jung S, Choe JH, Kim B, Yun H, Kruk ZA, Jo C. Effect of dietary mixture of gallic acid and linoleic acid on antioxidative potential and quality of breast meat from broilers. Meat Sci 2010;86:520-6.
  12. Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem 2006;96:254-60.
  13. Thring TS, Hili P, Naughton DP. Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complement Altern Med 2009;9:27.
  14. Aviram M, Dornfeld L. Pomegranate juice consumption inhibits serum angiotensin converting enzyme activity and reduces systolic blood pressure. Atherosclerosis 2001;158:195-8.
  15. Kishawy AT, Omar AE, Gomaa AM. Growth performance and immunity of broilers fed rancid oil diets that supplemented with pomegranate peel extract and sage oil. Jap J Vet Res 2016;64:S31-S38.
  16. Abdollahzadeh S, Mashouf R, Mortazavi H, Moghaddam M, Roozbahani N, Vahedi M. Antibacterial and antifungal activities of Punica granatum peel extracts against oral pathogens. J Dent (Tehran, Iran) 2011;8:1.
  17. Belkacem N, Djaziri R, El-Haci IA, Lahfa F, Boucherit K. Antihyperglycaemic effect of hydroalcoholic extract from Punica granatum L. peels in normal and streptozotocin-induced diabetic rats and its potent ${\alpha}$-amylase inhibitory. Der Pharma Chemica 2010;2:416-28.
  18. Ibrahium M. Efficiency of pomegranate peel extract as antimicrobial, antioxidant and protective agents. World J Agric Sci 2010;6:338-44.
  19. Fayed AM, Azoz A, Zedan AH, Basyony M. Effects of pomegranate peel as antioxidant supplementation on digestibility, blood biochemical and rabbit semen quality. Egy J Nutr and Feeds 2012;15:343-54.
  20. Harikrishnan R, Kim J-S, Kim M-C, Balasundaram C, Heo M-S. Pomegranate enriched diet enhances the hematology, innate immune response, and disease resistance in olive flounder against Philasterides dicentrarchi. Vet Parasitol 2012;187:147-156.
  21. Vantress C. Cobb broiler management guide. Siloam Springs, AR, USA: Cobb-Vantress; 2012.
  22. Wang Z, Pan Z, Ma H, Atungulu GG. Extract of phenolics from pomegranate peels. Open Food Sci J 2011;5:17-25.
  23. Hossin FLA. Effect of pomegranate (Punica granatum) peels and it's extract on obese hypercholesterolemic rats. Pak J Nutr 2009;8:1251-7.
  24. Singh R, Chidambara Murthy K, Jayaprakasha G. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem 2002;50:81-6.
  25. Lapornik B, Prosek M, Wondra AG. Comparison of extracts prepared from plant by-products using different solvents and extraction time. J Food Engineer 2005;71:214-22.
  26. Elsdon G. Edible oils and fats. London, UK: Ernest Benn, Ltd; 1926. pp. 79-80.
  27. AOAC. Official methods of analysis. Gaithersburg, MD, USA: AOAC International; 1984.
  28. Akoh CC. Food lipids: chemistry, nutrition, and biotechnology. Boca Raton, FL, USA: CRC press; 2017.
  29. Gray J. Measurement of lipid oxidation: a review. J Am Oil Chemist Soc 1978;55:539-546.
  30. AOAC. Official methods of analysis. Rockville, MD, USA: AOAC; 2000.
  31. Wagner DD, Furrow RD, Bradley BD. Subchronic toxicity of monensin in broiler chickens. Vet Pathol 1983;20:353-9.
  32. Brody S. Bioenergetics and growth; with special reference to the efficiency complex in domestic animals. Oxford, England: Reinhold; 1945.
  33. McDonald P. Animal nutrition. Pearson education; 2002.
  34. Finley PR, Schifman RB, Williams RJ, Lichti DA. Cholesterol in high-density lipoprotein: use of Mg2+/dextran sulfate in its enzymic measurement. Clin Chem 1978;24:931-3.
  35. Wahlefeld A, Bergmeyer H. Methods of enzymatic analysis. New York, NY, USA: Academic Press; 1974.
  36. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499-502.
  37. Schultz L. Methods in clinical chemistry. St Louis, MO, USA: The CV Mosby Co; 1987. pp. 742-6.
  38. Belitz H-D, Grosch W, Schieberle P. Meat. In: Food Chemistry. Berlin, Heidelberg, Germany: Springer; 2009. pp. 563-616.
  39. Mothershaw AS, Gaffer T, Kadim I, et al. Quality characteristics of broiler chicken meat on salt at different temperatures. Int J Food Prop 2009;12:681-90.
  40. Farag R, Abdelatif M, Emam S, Tawfeek L. Phytochemical screening and polyphenol constituents of pomegranate peels and leave juices. Agric Soil Sci 2014;1:86-93.
  41. Shunthwal J, Sheoran N. Influence of linseed oil feeding on performance and fatty acid composition of muscles in broiler chicks. Pharma Innov J 2017;6:268-73.
  42. Lei F, Zhang X, Wang W, et al. Evidence of anti-obesity effects of the pomegranate leaf extract in high-fat diet induced obese mice. Int J Obes 2007;31:1023-9.
  43. Osek M, Gorska A, Milczarek A, Swinarska R. Effect of soybean and linseed oil contents in mixtures for broiler chickens on the contents of triglycerides and cholesterol in serum and on meat quality. Птахівництво 2008;61:307-12.
  44. Zelenka J, Schneiderova D, Mrkvicova E, Dolezal P. The effect of dietary linseed oils with different fatty acid pattern on the content of fatty acids in chicken meat. Vet Med (Praha) 2008;53:77-85.
  45. Ross RG, Selvasubramanian S, Jayasundar S. Immunomodulatory activity of Punica granatum in rabbits-a preliminary study. J Ethnopharmacol 2001;78:85-87.