DOI QR코드

DOI QR Code

Association analysis of polymorphisms of G protein-coupled receptor 54 gene exons with reproductive traits in Jiaxing Black sows

  • Received : 2018.11.06
  • Accepted : 2018.12.18
  • Published : 2019.08.01

Abstract

Objective: The aim of this study was to detect single nucleotide polymorphisms (SNP) of G protein-coupled receptor 54 (GPR54) gene and explore association of this candidate gene with reproductive traits in Jiaxing Black sows. Methods: Six pairs of primers of the gene were designed to amplify all exons thus sequences of which were detected by means of direct sequencing and then SNP loci were scanned. The effects of SNPs on total number of piglets born (TNB), number of piglets born alive (NBA), number of still born piglets (NSB), and litter weight at birth (LWB) of Jiaxing Black sows were analyzed. Results: Three SNP loci, including T3739C, C3878T and T6789C, were identified via comparison of sequencing and two genotypes (AB, BB) at each SNP site were observed. T3739C resulted in the change of amino acid ($Leu{\rightarrow}Pro$) in corresponding protein, and C3878T resulted in synonymous mutation ($Ile{\rightarrow}Ile$). Statistical results demonstrated that allele B was the preponderant allele at the three SNP loci and Genotype BB was the preponderant genotype. Meanwhile, Chi-Square test of these three SNPs indicated that all mutation sites fitted in Hardy-Weinberg equilibrium (p>0.05). For GPR54-T3739C locus, Jiaxing Black sows with genotype BB had 1.23 TNB and 1.28 NBA (p<0.01) that were more than those with genotype AB, respectively. Jiaxing Black sows that had the first two parities with genotype BB had additional 2.23 TNB, 2.27 NBA (p<0.01), and 1.94 LWB (p<0.05) compared to those with genotype AB, respectively. However, for other two loci, no significant difference was found between TNB, NBA, NSB, and LWB, and different genotypes of Jiaxing Black sows. Conclusion: In conclusion, the polymorphisms of GPR54-T3739C locus were significantly associated to TNB, NBA, and LWB and could be used as a potential genetic marker to improve reproductive function of Jiaxing black sows.

References

  1. Lee DK, Nguyen T, O'Neill GP, et al. Discovery of a receptor related to the galanin receptors. FEBS Lett 1999;446:103-7. https://doi.org/10.1016/S0014-5793(99)00009-5 https://doi.org/10.1016/S0014-5793(99)00009-5
  2. D'Anglemont DTX, Fagg LA, Dixon JP, et al. Hypogonadotropic hypogonadism in mice lacking a functional Kiss1 gene. Proc Natl Acad Sci USA 2007;104:10714-9. https://doi.org/10.1073/pnas.0704114104 https://doi.org/10.1073/pnas.0704114104
  3. Dungan HM, Clifton DK, Steiner RA. Minireview: kisspeptin neurons as central processors in the regulation of gonadotropin-releasing hormone secretion. Endocrinology 2006;147:1154-8. https://doi.org/10.1210/en.2005-1282 https://doi.org/10.1210/en.2005-1282
  4. Kotani M, Detheux M, Vandenbogaerde A, et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. J Biol Chem 2001;276:34631-6. https://doi.org/10.1074/jbc.M104847200 https://doi.org/10.1074/jbc.M104847200
  5. Muir AI, Chamberlain L, Elshourbagy NA, et al. AXOR12, a novel human G protein-coupled receptor, activated by the peptide KiSS-1. J Biol Chem 2001;276:28969-75. https://doi.org/10.1074/jbc.M102743200 https://doi.org/10.1074/jbc.M102743200
  6. Navarro VM, Castellano JM, Fernandez-Fernandez R, et al. Developmental and hormonally regulated messenger ribonucleic acid expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus and potent luteinizing hormonereleasing activity of KiSS-1 peptide. Endocrinology 2004;145:4565-74. https://doi.org/10.1210/en.2004-0413 https://doi.org/10.1210/en.2004-0413
  7. An XP, Han P, Hou JX, et al. Molecular cloning and characterization of KISS1 promoter and effect of KISS1 gene mutations on litter size in the goat. Genet Mol Res 2013;12:4308-16. https://doi.org/10.4238/2013.February.28.13 https://doi.org/10.4238/2013.February.28.13
  8. El-Tarabany MS, Zaglool AW, El-Tarabany AA, Awad A. Association analysis of polymorphism in KiSS1 gene with reproductive traits in goats. Anim Reprod Sci 2017;180:92-9. https://doi.org/10.1016/j.anireprosci.2017.03.006 https://doi.org/10.1016/j.anireprosci.2017.03.006
  9. Maitra A, Sharma R, Ahlawat S, Tantia MS, Roy M, Prakash V. Association analysis of polymorphisms in caprine KiSS1 gene with reproductive traits. Anim Reprod Sci 2014;151:71-7. https://doi.org/10.1016/j.anireprosci.2014.09.013 https://doi.org/10.1016/j.anireprosci.2014.09.013
  10. Messager S, Chatzidaki EE, Ma D, et al. Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54. Proc Natl Acad Sci USA 2005;102:1761-6. https://doi.org/10.1073/pnas.0409330102 https://doi.org/10.1073/pnas.0409330102
  11. Shahab M, Mastronardi C, Seminara SB, Crowley WF, Ojeda SR, Plant TM. Increased hypothalamic GPR54 signaling: a potential mechanism for initiation of puberty in primates. Proc Natl Acad Sci USA 2005;102:2129-34. https://doi.org/10.1073/pnas.0409822102 https://doi.org/10.1073/pnas.0409822102
  12. Dhillo WS, Chaudhri OB, Patterson M, et al. Kisspeptin-54 stimulates the hypothalamic-pituitary gonadal axis in human males. J Clin Endocrinol Metab 2005;90:6609-15. https://doi.org/10.1210/jc.2005-1468 https://doi.org/10.1210/jc.2005-1468
  13. Greives TJ, Mason AO, Scotti MA, et al. Environmental control of kisspeptin: implications for seasonal reproduction. Endocrinology 2007;148:1158-66. https://doi.org/10.1210/en.2006-1249 https://doi.org/10.1210/en.2006-1249
  14. Funes S, Hedrick JA, Vassileva G, et al. The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system. Biochem Biophys Res Commun 2003;312:1357-63. https://doi.org/10.1016/j.bbrc.2003.11.066 https://doi.org/10.1016/j.bbrc.2003.11.066
  15. Herbison AE, de Tassigny X, Doran J, Colledge WH. Distribution and postnatal development of Gpr54 gene expression in mouse brain and gonadotropin-releasing hormone neurons. Endocrinology 2010;151:312-21. https://doi.org/10.1210/en.2009-0552 https://doi.org/10.1210/en.2009-0552
  16. Han SK, Gottsch ML, Lee KJ, et al. Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty. J Neurosci 2005;25:11349-56. https://doi.org/10.1523/JNEUROSCI.3328-05.2005 https://doi.org/10.1523/JNEUROSCI.3328-05.2005
  17. Irwig MS, Fraley GS, Smith JT, et al. Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat. Neuroendocrinology 2004;80:264-72. https://doi.org/10.1159/000083140 https://doi.org/10.1159/000083140
  18. Li S, Ren J, Yang G, Guo Y, Huang L. Characterization of the porcine Kisspeptins receptor gene and evaluation as candidate for timing of puberty in sows. J Anim Breed Genet 2008;125:219-27. https://doi.org/10.1111/j.1439-0388.2008.00732.x
  19. Seminara SB, Messager S, Chatzidaki EE, et al. The GPR54 gene as a regulator of puberty. N Engl J Med 2003;349:1614-27. https://doi.org/10.1056/NEJMoa035322 https://doi.org/10.1056/NEJMoa035322
  20. de Roux N, Genin E, Carel JC, Matsuda F, Chaussain JL, Milgrom E. Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proc Natl Acad Sci USA 2003;100:10972-6. https://doi.org/10.1073/pnas.1834399100 https://doi.org/10.1073/pnas.1834399100
  21. Semple RK, Achermann JC, Ellery J, et al. Two novel missense mutations in G protein-coupled receptor 54 in a patient with hypogonadotropic hypogonadism. J Clin Endocr Metab 2005;90:1849-55. https://doi.org/10.1210/jc.2004-1418 https://doi.org/10.1210/jc.2004-1418
  22. Tenenbaum-Rakover Y, Commenges-Ducos M, Iovane A, Aumas C, Admoni O, de Roux N. Neuroendocrine phenotype analysis in five patients with isolated hypogonadotropic hypogonadism due to a L102P inactivating mutation of GPR54. J Clin Endocrinol Metab 2007;92:1137-44. https://doi.org/10.1210/jc.2006-2147 https://doi.org/10.1210/jc.2006-2147
  23. Teles MG, Bianco SD, Brito VN, et al. A GPR54-activating mutation in a patient with central precocious puberty. N Engl J Med 2008; 358:709-15. https://doi.org/10.1056/NEJMoa073443 https://doi.org/10.1056/NEJMoa073443
  24. Luan X, Yu H, Wei X, et al. GPR54 polymorphisms in Chinese girls with central precocious puberty. Neuroendocrinology 2007;86:77-83. https://doi.org/10.1159/000107511 https://doi.org/10.1159/000107511
  25. Tang QQ, Chu MX, Cao GL, et al. Association between polymorphism of GPR54 gene and litter size in Small Tail Han sheep. Livest Sci 2012;143:97-103. https://doi.org/10.1016/j.livsci.2011.09.005 https://doi.org/10.1016/j.livsci.2011.09.005
  26. Chu MX, Xiao CT, Feng T, et al. Polymorphisms of KiSS-1 and GPR54 genes and their relationships with litter size in sheep. Mol Biol Rep 2012;39:3291-7. https://doi.org/10.1007/s11033-011-1097-3 https://doi.org/10.1007/s11033-011-1097-3
  27. Maitra A, Sharma R, Ahlawat S, Tantia MS. Novel genetic polymorphisms in caprine GPR54 gene associated with reproductive functions. Indian J Anim Sci 2014;84:1196-201.
  28. Feng T, Zhao YZ, Chu MX, et al. Association between sexual precocity and alleles of KISS-1 and GPR54 genes in goats. Anim Biotechnol 2009;20:172-6. https://doi.org/10.1080/10495390903004493 https://doi.org/10.1080/10495390903004493
  29. Cao GL, Chu MX, Fang L, Feng T, Di R, Li N. Analysis on DNA sequence of GPR54 gene and its association with litter size in goats. Mol Biol Rep 2011;38:3839-48. https://doi.org/10.1007/s11033-010-0499-y https://doi.org/10.1007/s11033-010-0499-y