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Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis

Kim, Juyun;Kim, Namgyu;Mannaa, Mohamed;Lee, Hyun-Hee;Jeon, Jong-Seong;Seo, Young-Su

  • Received : 2019.02.07
  • Accepted : 2019.03.20
  • Published : 2019.06.01

Abstract

In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.

Keywords

Burkholderia glumae;virulence;yeast two hybrid analysis

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)