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A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant

Jatoi, Ghulam Hussain;Lihua, Guo;Xiufen, Yang;Gadhi, Muswar Ali;Keerio, Azhar Uddin;Abdulle, Yusuf Ali;Qiu, Dewen

  • Received : 2018.12.08
  • Accepted : 2019.03.28
  • Published : 2019.06.01

Abstract

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

Keywords

Botrytis cinereal;Brevibacillus laterosporus A60;novel protein;PeBL2;protein elicitor activity