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Development and Validation of a Perfect KASP Marker for Fusarium Head Blight Resistance Gene Fhb1 in Wheat

Singh, Lovepreet;Anderson, James A;Chen, Jianli;Gill, Bikram S;Tiwari, Vijay K;Rawat, Nidhi

  • Received : 2019.01.27
  • Accepted : 2019.03.31
  • Published : 2019.06.01

Abstract

Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFT-based Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering results were compared with gelbased gene specific markers and the widely used linked STS marker, UMN10. Eight disagreements were found between PFT_KASP and UMN10 assays among the tested lines. Based on the genotyping and sequencing of genes in the Fhb1 region, these genotypes were found to be common with a previously characterized susceptible haplotype. Therefore, our results indicate that PFT_KASP is a perfect diagnostic marker for Fhb1 and would be a valuable tool for introgression and pyramiding of FHB resistance in wheat cultivars.

Keywords

Fhb1;Fusarium head blight;KASP assay;pore-forming toxin-like (PFT);Wheat

Acknowledgement

Supported by : USDA National Institute of Food and Agriculture, Maryland Agricultural Experiment Station via MAES