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A report of four unrecorded Proteobacteria species isolated from soil in Korea

  • Lee, Ki-Eun (Biological Resources Utilization Department, National Institute of Biological Resources) ;
  • Kim, Ju-Young (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Jang, Jun Hwee (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Maeng, Soohyun (Department of Public Health Sciences, Graduate School, Korea University) ;
  • Srinivasan, Sathiyaraj (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Subramani, Gayathri (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kang, Myung-Suk (Biological Resources Utilization Department, National Institute of Biological Resources)
  • Received : 2018.06.22
  • Accepted : 2019.01.07
  • Published : 2019.05.31

Abstract

In 2015 and 2017, the National Institute of Biological Resources has isolated four unrecorded prokaryotic species designated as R-1-5, R-2-13, R-2-1, and R-1-8 from the peatland soil of Yongneup. Phylogenetic analysis based on 16S rRNA gene sequence similarity determined the four strains (R-1-5, R-2-13, R-2-1, R-1-8) were most closely related to Curvibacter lanceolatus (99.93%), Massilia brevitalea (98.7%), Pseudomonas lini (99.54%), and Pseudomonas vancouverensis (99.93%), respectively. The four unrecorded strains belong to the phylum Proteobacteria, in which the genera Curvibacter and Massilia are assigned to the class Betaproteobacteria, and the genus Pseudomonas to the class Gammaproteobacteria. Since there are no publications or official reports on these four strains, these four species are new records to Korea. The strains were further characterized by Gram reaction, colony and cell morphology, basic biochemical properties, and phylogenetic position. Descriptive information of the four unrecorded species is provided.

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Fig. 1. Transmission electron micrographs of the strains isolated in this study. Strains: (A) Curvibacter lanceolatus R-1-5; (B) Massilia brevitalea R-2-13; (C) Pseudomonas lini R-2-1; and (D) Pseudomonas vancouverensis R-1-8.

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Fig. 2. Neighbor-joining phylogenetic tree based on 16S rRNA sequences shows the relationship between the strains isolated in this study and their relatives in the genera Curvibacter (A), Massilia (B) and Pseudomonas (C). Bootstrap values (>70%) are shown above nodes for the neighbor-joining. Scale bar: 0.01 changes per nucleotide.

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Fig. 2. Continued.

Table 1. Summary of strains isolatèd belonging to the Proteobacteria and their taxonomic affliations.

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Acknowledgement

Supported by : National Institute of Biological Resources

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