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The Effect of Dilution with 10% Dextrose Solution on the Potency of Botulinum Toxin A

  • Ham, Jong Wook (Department of Orthodontics and Cosmetic Dentistry, Dr. Ham's Dental and Botulinum Toxin Clinic) ;
  • Kwon, Jeong-Seung (Department of Orofacial Pain and Oral Medicine, Yonsei Dental Hospital, Yonsei University College of Dentistry) ;
  • Cho, Eunae Sandra (Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry) ;
  • Choi, Jong Hoon (Department of Orofacial Pain and Oral Medicine, Yonsei Dental Hospital, Yonsei University College of Dentistry)
  • Received : 2019.01.18
  • Accepted : 2019.02.02
  • Published : 2019.03.30

Abstract

Purpose: The aim of this study was to compare the potency-stabilizing effects of two different diluents of botulinum toxin A (10% dextrose solution and 0.9% saline). Methods: A mouse lethality bioassay was undertaken. Ninety mice were divided into experimental and control groups which received varying dosages in subgroups of 10. The experimental group was injected with botulinum toxin A diluted with 10% dextrose solution and the control group was injected with botulinum toxin A diluted with 0.9% saline. A 72 hours after intraperitoneal injection, the number of dead mice was counted to confirm median lethal dose ($LD_{50}$) of each group. Results: The value of $LD_{50}$ in the experimental group was approximately 0.131 mL (1.31 U) and the value of $LD_{50}$ in the control group was approximately 0.107 mL (1.07 U). The potency preservation rate of the experimental group was estimated to be 93.5% and that of the control group was estimated to be 76.3%. Conclusions: Dilution with 10% dextrose solution displayed less potency loss than 0.9% saline.

Keywords

Botulinum toxins;Type A;Glucose;Saline solution

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Fig. 1. LD50 calculation of control group. Using regression equation Y=8.6999X+4.7389, X is calculated when Y is 5, and anti-LogX (Y=5) is 1.07 U.

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Fig. 2. LD50 calculation of experimental group. Using regression equation Y=7.4655X+4.1243, X is calculated when Y is 5, and anti- LogX (Y=5) is 1.31 U.

Table 1. Mortality rate after botulinum toxin A injection with or without additional dextrose solution injection

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Table 2. Number of dead mouse and mortality rate in the control group

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Table 3. Conversions for LD50 in the control group

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Table 5. Conversions for LD50 in the experimental group

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Table 4. Number of dead mice and mortality rate in the experimental group

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Acknowledgement

Supported by : Yonsei University College of Dentistry

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