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뽕나무(Morus alba)와 꾸지뽕나무(Cudrania tricuspidata)의 부위에 따른 항산화 활성 및 3T3-L1세포 지방축적 억제 효과

Antioxidative Activities and Inhibitory Effects on Lipid Accumulation of Extracts from Different Parts of Morus alba and Cudrania tricuspidata

  • 김건희 (덕성여자대학교 식품영양학과) ;
  • 김은향 (덕성여자대학교 식품영양학과)
  • Kim, Gun-Hee (Dept. of Food and Nutrition, Duksung Women's University) ;
  • Kim, Eunhyang (Dept. of Food and Nutrition, Duksung Women's University)
  • 투고 : 2019.03.13
  • 심사 : 2019.04.17
  • 발행 : 2019.04.30

초록

In this study, we examined antioxidative effects and the anti-adipogenesis effect of different parts of Cudrania tricuspidata (C), and Morus alba (M). Total polyphenol contents were highest in M-root ($34.56{\pm}0.045mg\;GAE/g$), and there was no significant difference, between C-root and M-leaf. Total flavonoid contents of C-root were highest ($23.07{\pm}0.004mg\;QE/g$). To examine antioxidant activities of C and M extracts, DPPH and ABTS radical scavenging activity, and FRAP assay, was used. Results show that antioxidant activities of C and M extracts increased, in a dose-dependent manner. Adipocytes are generated by preadipocyte differentiation, during adipogenesis. Matured adipocytes accumulate in abnormal and cause obesity. We investigated effects of leaf and root extracts of C and M, on lipid accumulation, in 3T3-L1 adipocytes. Changes in cell morphology, and degrees of lipid accumulation in adipocytes, were evaluated by Oil Red O staining. Root extracts of C and M, reduced lipid content in a dose-dependent manner. Therefore, root extracts of C and M, may be good candidates for managing obesity.

키워드

Cudrania tricuspidata;Morus alba;antioxidative activity;3T3-L1;adipogenesis

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Fig. 1. DPPH radical scavenging activity of the extracts from different parts of Cudrania tricuspidata.

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Fig. 2. DPPH radical scavenging activity of the extracts from different parts of Morus alba.

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Fig. 3. ABTS radical scavenging activity of the extracts from different parts of Cudrania tricuspidata.

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Fig. 4. ABTS radical scavenging activity of extracts from different parts of Morus alba.

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Fig. 5. Ferric reducing antioxidant power (FRAP) of the extracts from different parts of Cudrania tricuspidata.

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Fig. 6. Ferric reducing antioxidant power (FRAP) of extracts from different parts of Morus alba.

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Fig. 7. Cell viability of the leaf extracts of Cudrania tricuspidata and Morus alba on 3T3-L1 preadipocytes.

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Fig. 8. Cell viability of the root extracts of Cudrania tricuspidata and Morus alba on 3T3-L1 preadipocytes.

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Fig. 9. Observation of lipid droplets in 3T3-L1 adipocytes treated with leaf extracts of Cudrania tricuspidata and Morus alba.

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Fig. 10. Lipid accumulation of leaf extracts of Cudrania tricuspidata and Morus alba in 3T3-L1 adipocytes.

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Fig. 11. Observation of lipid droplets in 3T3-L1 adipocytes treated with root extracts of Cudrania tricuspidata and Morus alba.

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Fig. 12. Lipid accumulation of root extracts of Cudrania tricuspidata and Morus alba in 3T3-L1 adipocytes.

Table 1. Yield, total phenolic contents and total flavonoid contents of extracts from different parts of Cudrania tricuspidata and Morus alba

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과제정보

연구 과제 주관 기관 : 덕성여자대학교

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