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Preventive Effect of the Water Extract of Agrimonia pilosa Ledeb and Micronucleus Assay-Based Evaluation of Genotoxicity in Gastritis Animal Models

동물모델을 이용한 짚신나물 물 추출물의 위염 예방효과 및 유전독성 평가

Nho, Jong Hyun;Jang, Ji Hun;Lee, Hyun Joo;Yang, Beodul;Woo, Kyeong Wan;Kim, A Hyeon;Seo, Jae Wan;Hwang, Tae Yeon;Cho, Hyun Woo;Jung, Ho Kyung
노종현;장지훈;이현주;양버들;우경완;김아현;서재완;황태연;조현우;정호경

  • Received : 2019.03.04
  • Accepted : 2019.04.08
  • Published : 2019.04.30

Abstract

Background: Agrimonia pilosa Ledeb has been used as a traditional medicine for the treatment of hematuresis and uterine bleeding in Korea. It has been reported to have anti-obesity, anti-diabetes and anti-inflammaotry effect by regulating the inflammatory signaling pathway. However, the preventive effect of Agrimonia pilosa Ledeb on gastritis has not been elucidated. Thus, in the present study, we evaluated the effects of the water extract of Agrimonia pilosa Ledeb (APW) using HCl/EtOH-induced gastritis rat models. Method and Results: Gastritis was induced in rats by HCl/EtOH administration. The rats in each group were orally administered with two doses of APW (100 and 500 mg/kg). Omeprazole was used as a positive control drug. An enzyme-linked immunosorbent assay (ELISA) was used to measure the prostaglandin $E_2$ ($PGE_2$) levels in stomach. The treatment with 500 mg/kg APW reduced the gastric ulcer area. The APW treatment prevented a decreased in $PGE_2$ concentration induced by HCl/EtOH in rats. In the micronucleus test, the ratio of micronucleated polychromatic erythrocytes to polychromatic erythrocytes showed no significant change in the APW-treated group compared with the control group. Conclusions: These results indicate that APW could be used to prevent the gastritis caused by the HCl/EtOH-induced damage to stomach lining. In addition, the APW treatment showed no significant change in results of the micronucleus test. However, further experiments are required to determine how APW influenced the secretion of mucus and gastric acid using the chromosome aberration test and bacterial reverse mutation assay.

Keywords

Agrimonia pilosa Ledeb;Gastritis;Micronucleus Test

Acknowledgement

Supported by : 보건복지부