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Changes of Free Amino acid in PDB Medium by Lentinula edodes (Sanjo 701ho) Cultivation period

표고버섯 균사체 배양기간에 따른 PDB 배지 내 유리 아미노산 변화

  • Oh, Tae-Seok (Department of plant Resources, Kongju National University) ;
  • Park, Youn-Jin (Green Manure and Legumes Resource Center, Kongju National University) ;
  • Kim, Tae-Kwon (Green Manure and Legumes Resource Center, Kongju National University) ;
  • Ann, Seoung-Won (Department of Horticultural Science, Kongju National University) ;
  • Kim, Chang-Ho (Department of plant Resources, Kongju National University) ;
  • Cho, Yong-Koo (Department of plant Resources, Kongju National University) ;
  • Jang, Myoung-Jun (Department of plant Resources, Kongju National University)
  • 오태석 (공주대학교 식물자원학과) ;
  • 박윤진 (공주대학교 두과녹비자원연구센터) ;
  • 김태권 (공주대학교 두과녹비자원연구센터) ;
  • 안승원 (공주대학교 원예학과) ;
  • 김창호 (공주대학교 식물자원학과) ;
  • 조용구 (공주대학교 식물자원학과) ;
  • 장명준 (공주대학교 식물자원학과)
  • Received : 2019.03.22
  • Accepted : 2019.04.15
  • Published : 2019.04.30

Abstract

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of $9,889{\pm}3{\mu}g/L$ to $12,909{\pm}2{\mu}g/L$ at 45 days and $29,256{\pm}4{\mu}g/L$ at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to $83,751{\pm}2{\mu}g/L$ after 45 days from its initial value of $161,787{\pm}1{\mu}g/L$ and to $79,055{\pm}7{\mu}g/L$ at 90 days.

Keywords

Cultivation period;Lentinula edodes;Free amino acid

Acknowledgement

Supported by : 한국연구재단

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