Fabrication of enzymatic biosensor based on the poly(3-thiophenecarboxylic acid-co-thiophene) polymer as electron-transfer materials

  • Kim, Soo-Yeoun (Department of Health Cosmetic Science, Dongduk Women's University) ;
  • Jo, Hyeon-Jin (Department of Chemistry, Hannam University) ;
  • Choi, Seong-Ho (Department of Chemistry, Hannam University)
  • Received : 2019.01.28
  • Accepted : 2019.03.29
  • Published : 2019.03.31


We fabricated glucose oxidase (GOx)-modified biosensor for detection of glucose by physical immobilization of GOx after electrochemical polymerization of the conductive mixture monomers of the 3-thiophenecarboxylic acid (TCA) and thiophene (Th) onto ITO electrode in this study. We confirmed the successfully fabrication of GOx-modified biosensor via FT-IR spectroscopy, SEM, contact angle, and cyclic voltammetry. The fabricated biosensor has the detection limit of $0.1{\mu}M$, the linearity of 0.001-27 mM, and sensitivity of $38.75mAM^{-1}cm^{-2}$, respectively. The fabricated biosensor exhibits high interference effects to dopamine, ascorbic acid, and L-cysteine, respectively. From these results, the fabricated GOx-modified biosensor with long linearity and high sensitivity could be used as glucose sensor in human blood sample.

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Fig. 1. Preparation of GOx-midified biosensor based on poly(3-thiophenecarboxylic acid, TCA), poly(thiophene, TCA), and poly(TCA-co-Th).

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Fig. 2. Electrochemical polymerization of the TCA, Th and the mixture of TCA/Th in acetonitrile with 10mM[N(Bu)4]+[BF4]- at scan rate 0.075V/S(see, Table 1).

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Fig. 3. Electrochemical polymerization mechanism of thiophene derivatives in acetonitrile with 10mM [NCH3CH2CH2CH2]+[BF4]- as electrolyte at scan rate 75mV/s.

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Fig. 4. Contact angles of bare ITO, No. 1, 2, 3, 4 and 5 (see, Table 1).

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Fig. 5. FT-IR spectra of No. 1, No. 2 and No. 4 (see, Table 1).

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Fig. 6. Cross-section SEM images of poly(TCA-co-Th)/ITO electrode (see, Table 1).

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Fig. 7. Cyclic voltammograms of 1mM K3Fe (CN) 6 and K4Fe (CN) 6 (1/1, mol-%) using poly(TCA), poly(Th), and poly(TCA-co-Th)-modified ITO electrode in 0.1M KCl.

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Fig. 8. Cyclic voltammograms of glucose in 0.1 M PBS (pH 6.8) using GOx-modified biosensor (No. 4) at a scan rate of 100 mV/s.

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Fig. 9. Chronoamperometry electrochemical response according to glucose concentration using GOx biosensor (No. 5) in 0.1 M PBS solution at a -0.175 V potential.

Table 1. Electrochemical polymerization condition of the TCA, Th, and the mixture of TCA/Th a)

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Table 2. Interference effect of various compounds on the assay of glucose using GOx-modified biosensor (No. 4)

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Table 3. Comparison of the reported glucose biosensors

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Supported by : Hannam University


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