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Antimicrobial activity of fraction mixture of ethanol extracts from Eucalyptus globulus, Yucca recurvifolia, and Melaleuca alternifolia against several human skin microbes

유칼립투스, 유카와 차나무의 추출분획 혼합물의 여러 인간 피부 상재균에 대한 항균활성

  • Lee, Da-Sol (Department of Biological Sciences, Kangwon National University) ;
  • Hong, In Kee (R&D Center, Radiant Ltd.) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • Received : 2019.01.25
  • Accepted : 2019.02.20
  • Published : 2019.03.31

Abstract

This study was carried out to evaluate antimicrobial effects of a mixture of resin fractionated ethanol extract of Eucalyptus globulus, Yucca recurvifolia, and tea tree (Melaleuca alternifolia). The plant fraction mixture showed low minimum inhibitory concentration (0.24~3.32 mg/ml) against several bacteria and yeast that usually used as the target skin microbes in a cosmetic industry, and it was more effective than antibiotics, triclosan and ampicillin. In a time-kill assay the plant fraction mixture reduced more than 92% of microbial populations during 4 h, and significantly increased leakage of nucleotides from all microorganisms tested. Antimicrobial effect of the plant fraction mixture was not affected by divalent cation ($Mg^{2+}$ and $Ca^{2+}$). These results suggest that the fraction mixture of ethanol extracts of E. globulus, Y. recurvifolia, and M. alternifolia may be utilized as an efficient preservative in cosmetics to prevent contamination by human skin microbes.

Keywords

Eucalyptus;Yucca;antimicrobial activity;human skin microbes;tea tree

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Fig. 1. Growth of C. albicans ATCC10231 (A) B. subtilis ATCC19659 (B) S. aureus ATCC6538 (C) P. aeruginosa KCTC2513 (D) and E. coli ATCC8739 (E) in the presence of plant fraction mixture (Yucca, Eucalyptus, tea tree) and antibiotics (◇, control; X, plant fraction mixture; △, triclosan or ampicillin).

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Fig. 2. Nucleotide leakage of C. albicans ATCC10231 (A) B. subtilis ATCC19659 (B) S. aureus ATCC6538 (C) P. aeruginosa KCTC2513 (D) and E. coli ATCC8739 (E) in the presence of plant fraction mixture (Yucca, Eucalyptus, tea tree) and antibiotics (◇, control; △, plant fraction; X, triclosan or ampicillin).

Table 1. Minimum inhibitory concentration of antibiotics and plant fraction mixture against several human skin microbes

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Table 2. Effect of divalent cation on the antimicrobial activity of plant fraction mixture

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Acknowledgement

Supported by : 강원대학교

References

  1. Aiyegoro OA, Afolayan AJ, and Okoh AI. 2009. Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria. Biol. Res. 42, 327-338.
  2. Al-Ani I, Zimmermann S, Reichlinga J, and Wink M. 2015. Pharmacological synergism of bee venom and melittin with antibiotics and plant secondary metabolites against multi-drug resistant microbial pathogens. Phytomedicine 22, 245-255.
  3. Alhanout K, Malesinki S, Vidal N, Peyrot V, Rolain JM, and Brunel JM. 2010. New insights into the antibacterial mechanism of action of squalamine. J. Antimicrob. Chemother. 65, 1688-1693.
  4. Arunachalam K, Ascencio SD, Soares IM, Aguiar RWS, da Silva LI, de Oliveira RG, Balogun SO, and de Oliveira Martins DT. 2016. Gallesia integrifolia (Spreng.) Harms: In vitro and in vivo antibacterial activities and mode of action. J. Ethnopharmacol. 184, 128-137. https://doi.org/10.1016/j.jep.2016.03.005
  5. Ayed HB, Maalej H, Hmidet N, and Nasri M. 2015. Isolation and biochemical characterisation of a bacteriocin-like substance produced by Bacillus amyloliquefaciens An6. J. Global Antimicrob. Resist. 3, 255-261. https://doi.org/10.1016/j.jgar.2015.07.001
  6. Behravan J, Bazzaz BSF, and Malaekeh P. 2005. Survey of bacteriological contamination of cosmetic creams in Iran. Int. J. Dermatol. 44, 482-485.
  7. Carson CF, Hammer KA, and Riley TV. 2006. Melaleuca alternifolia (tea tree) oil: a review of antimicrobial and other medicinal properties. Clin. Microbiol. Rev. 19, 50-62.
  8. Hanckock RE, Farmer SW, Li ZS, and Poole K. 1991. Interaction of aminoglycosides with the outer membranes and purified lipopolysaccharide and OmpF porin of Escherichia coli. Antimicrob. Agents Chemother. 35, 1309-1311. https://doi.org/10.1128/AAC.35.7.1309
  9. Jackson C, Agboke A, and Nwoke V. 2009. In vitro evaluation of antimicrobial activity of combinations of nystatin and Euphorbia hirta leaf extract against Candida albicans by the checkerboard method. J. Med. Plant. Res. 3, 666-669.
  10. Jayaraman P, Sakharkar MK, Lim CS, Tang T, and Sakharkar KR. 2010. Activity and interactions of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro. Int. J. Biol. Sci. 6, 556-568.
  11. Kokura S, Handa O, Takagi T, Ishikawa T, Naito Y, and Yoshikawa T. 2010. Silver nanoparticles as a safe preservative for use in cosmetics. Nanomedicine 6, 570-574. https://doi.org/10.1016/j.nano.2009.12.002
  12. Ku JE, Han HS, and Song JH. 2013. The recent trend of the natural preservative used in cosmetics. Korean J. Aesthet. Cosmetol. 11, 835-844.
  13. Lahmar A, Bedoui A, Mokdad-Bzeouich I, Dhaoui Z, Kalboussi Z, Cheraif I, Ghedira K, and Chekir-Ghedira L. 2017. Reversal of resistance in bacteria underlies synergistic effect of essential oils with conventional antibiotics. Microb. Pathog. 106, 50-59.
  14. Lee JH. 2017. Master thesis. A study of microbiological contamination after use of lip makeup tester. Sookmyung Women's University, Seoul, Korea.
  15. Lou Z, Wang H, Zhu S, Ma C, and Wang Z. 2011. Antibacterial activity and mechanism of action of chlorogenic acid. J. Food Sci. 76, 398-403.
  16. Lundov MD and Zachariae C. 2008. Recalls of microbiologically contaminated cosmetics in EU from 2005 to May 2008. Int. J. Cosmet. Sci. 30, 471-474.
  17. Pereira V, Dias C, Vasconcelos MC, Rosa E, and Saavedra MJ. 2014. Antibacterial activity and synergistic effects between Eucalyptus globulus leaf residues (essential oils and extracts)and antibiotics against several isolates of respiratory tract infections (Pseudomonas aeruginosa). Ind. Crops Prod. 52, 1-7.
  18. Sanchez E, Heredia N, and Garcia S. 2005. Inhibition of growth and mycotoxin production of Aspergillus flavus and Aspergillus parasiticus by extracts of Agave species. Int. J. Food Microbiol. 98, 271-279.
  19. Sahalan AZ, Aziz AHA, Lian HH, and Ghani MKA. 2013. Divalent cations ($Mg^{2+}$, $Ca^{2+}$) protect bacterial outer membrane damage by polymyxin B. Sains Malays. 42, 301-306.
  20. Stefanovic O, Comic L, Stanojevic D, and Solujic-Sukdolak S. 2009. antibacterial activity of Aegopodium podagraria L. extracts and interaction between extracts and antibiotics. Turk. J. Biol. 33, 145-150.
  21. White IR and Groot AC. 2006. Cosmetics and skin care products, pp. 493-506. In Frosch PJ, Menn T, and Lepoittevin JP. (eds.), Contact Dermatitis, 4th ed. Springer, Berlin, Germany.
  22. Zou YY, Jung LS, Lee SH, Kim SK, Cho YJ, and Ahn JH. 2012. Enhanced antimicrobial activity of nisin in combination with allyl isothiocyanate against Listeria monocytogenes, Staphylococcus aureus, Salmonella Typhimurium and Shigella boydii. Int. J. Food Sci. Technol. 48, 324-333.
  23. Zubair M, Rasool N, Mansha A, Anjum F, Iqbal M, Mushtaq M, and Shahid M. 2013. Antioxidant, antibacterial, antifungal activities and phytochemical analysis of dagger (Yucca aloifolia) leaves extracts. J. Med. Plant Res. 7, 243-249.