Antimicrobial activity of fraction mixture of ethanol extracts from Eucalyptus globulus, Yucca recurvifolia, and Melaleuca alternifolia against several human skin microbes

유칼립투스, 유카와 차나무의 추출분획 혼합물의 여러 인간 피부 상재균에 대한 항균활성

  • Lee, Da-Sol (Department of Biological Sciences, Kangwon National University) ;
  • Hong, In Kee (R&D Center, Radiant Ltd.) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • Received : 2019.01.25
  • Accepted : 2019.02.20
  • Published : 2019.03.31


This study was carried out to evaluate antimicrobial effects of a mixture of resin fractionated ethanol extract of Eucalyptus globulus, Yucca recurvifolia, and tea tree (Melaleuca alternifolia). The plant fraction mixture showed low minimum inhibitory concentration (0.24~3.32 mg/ml) against several bacteria and yeast that usually used as the target skin microbes in a cosmetic industry, and it was more effective than antibiotics, triclosan and ampicillin. In a time-kill assay the plant fraction mixture reduced more than 92% of microbial populations during 4 h, and significantly increased leakage of nucleotides from all microorganisms tested. Antimicrobial effect of the plant fraction mixture was not affected by divalent cation ($Mg^{2+}$ and $Ca^{2+}$). These results suggest that the fraction mixture of ethanol extracts of E. globulus, Y. recurvifolia, and M. alternifolia may be utilized as an efficient preservative in cosmetics to prevent contamination by human skin microbes.


Eucalyptus;Yucca;antimicrobial activity;human skin microbes;tea tree

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Fig. 1. Growth of C. albicans ATCC10231 (A) B. subtilis ATCC19659 (B) S. aureus ATCC6538 (C) P. aeruginosa KCTC2513 (D) and E. coli ATCC8739 (E) in the presence of plant fraction mixture (Yucca, Eucalyptus, tea tree) and antibiotics (◇, control; X, plant fraction mixture; △, triclosan or ampicillin).

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Fig. 2. Nucleotide leakage of C. albicans ATCC10231 (A) B. subtilis ATCC19659 (B) S. aureus ATCC6538 (C) P. aeruginosa KCTC2513 (D) and E. coli ATCC8739 (E) in the presence of plant fraction mixture (Yucca, Eucalyptus, tea tree) and antibiotics (◇, control; △, plant fraction; X, triclosan or ampicillin).

Table 1. Minimum inhibitory concentration of antibiotics and plant fraction mixture against several human skin microbes

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Table 2. Effect of divalent cation on the antimicrobial activity of plant fraction mixture

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Supported by : 강원대학교


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