Isolation and characterization of 6 unrecorded Pseudomonas spp. from Korean soil

토양에서 분리한 국내 미기록종 Pseudomonas 속 6종의 생화학적 특성과 계통 분류

  • Kim, Hyun-Joong (Institute of Life Sciences and Resources Graduate School of Biotechnology, Kyung Hee University) ;
  • Jung, You-Jung (Biological Resources Research Department, National Institute of Biological Resources) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources Graduate School of Biotechnology, Kyung Hee University) ;
  • Hur, Moonsuk (Biological Resources Research Department, National Institute of Biological Resources)
  • 김현중 (경희대학교 생명과학대학 식품생명공학 전공) ;
  • 정유정 (국립생물자원관 생물자원연구부 미생물자원과) ;
  • 김해영 (경희대학교 생명과학대학 식품생명공학 전공) ;
  • 허문석 (국립생물자원관 생물자원연구부 미생물자원과)
  • Received : 2018.11.30
  • Accepted : 2018.12.19
  • Published : 2019.03.31


In 2017, as a study to discover indigenous prokaryotic species in Korea, a total of 6 bacterial strains assigned to the genus Pseudomonas were isolated from soil. From the high 16S rRNA gene sequence similarity (${\geq}99.5%$) and phylogenetic analysis with closely related species, the isolated strains were identified as independent Pseudomonas species which were unrecorded in Korea. The six Pseudomonas species were Pseudomonas mandelii, P. canadensis, P. thivervalensis, P. jessenii, P. lurida, and P. brenneri. Gram reaction, culture conditions, colony and cell morphology, basic physiological and biochemical characteristics are described in the species description section.


Pseudomonas;16S rRNA gene sequencing;gammaproteobacteria;soil;unrecorded species

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Fig. 1. Phylogenetic tree of Pseudomonas spp. based on the 16S rRNA gene sequence analysis using the neighbor-joining method showing the position of the newly isolated Pseudomonas strains (highlighted in bold) compared with the closely reference Pseudomonas strains.

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Fig. 2. Transmission electron micrograph of isolated Pseudomonas spp.

Table 1. Pseudomonas strains isolated from soil in this study

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Table 2. Physiological and biochemical characteristics and differential carbohydrate assimilation patterns of isolated Pseudomonas spp. using API 20NE (2.1) kit, API ZYM kit, and API 32GN kit

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Supported by : 국립생물자원관


  1. Achouak W, Sutra L, Heulin T, Meyer JM, Fromin N, Degraeve S, Christen R, and Gardan L. 2000. Pseudomonas brassicacearum sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana. Int. J. Syst. Evol. Microbiol. 50, 9-18.
  2. Anzai Y, Kim H, Park JY, Wakabayashi H, and Oyaizu H. 2000. Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int. J. Syst. Evol. Microbiol. 50, 1563-1589.
  3. Baida N, Yazourh A, Singer E, and Izard D. 2001. Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters. Res. Microbiol. 152, 493-502.
  4. Behrendt U, Ulrich A, Schumann P, Meyer JM, and Sproer C. 2007. Pseudomonas lurida sp. nov., a fluorescent species associated with the phyllosphere of grasses. Int. J. Syst. Evol. Microbiol. 57, 979-985.
  5. Berdy JJ. 2005. Bioactive microbial metabolites. J. Antibiot. 58, 1-26.
  6. Jaspers E and Overmann J. 2004. Ecological significance of microdiversity: Identical 16S rRNA gene sequences can be found in bacteria with highly divergent genomes and ecophysiologies. Appl. Environ. Microbiol. 70, 4831-4839.
  7. Kimura M. 1983. Rare variant alleles in the light of the neutral theory. Mol. Biol. Evol. 1, 84-93.
  8. Lane DJ. 1991. 16S/23S rRNA sequencing, pp. 115-175. In Stackebrandt E and Goodfellow M (eds.), Nucleic acid techniques in bacterial systematics. John Wiley and Sons, New York, USA.
  9. Lee YM, Kim GH, Jung YJ, Choe CD, Yim JH, Lee HK, and Hong SG. 2012. Polar and alpine microbial collection (PAMC): a culture collection dedicated to polar and alpine microorganisms. Polar Biol. 35, 1433-1438.
  10. Lopez JR, Dieguez AL, Doce A, De la Roca E, De la Herran R, Navas JI, Toranzo AE, and Romalde JL. 2012. Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneate (Moreau). Int. J. Syst. Evol. Microbiol. 62, 874-882.
  11. Mesbah M, Premachandran U, and Whitman WB. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Evol. Microbiol. 39, 159-167.
  12. Moore LR, Rocap G, and Chisholm SW. 1998. Physiology and molecular phylogeny of coexisting Prochlorococcus ecotypes. Nature 393, 464-467.
  13. Mulet M, Lalucat J, and Garcia-Valdes E. 2010. DNA sequence-based analysis of the Pseudomonas species. Environ. Microbiol. 12, 1513-1530.
  14. Palleroni NJ. 1984. Genus I. Pseudomonas. pp. 141-199. In Krieg NR and Holt JG (eds.), Bergey's manual of systematic bacteriology, Vol. 2. The Williams & Wilkins Co., Baltimore, MD, USA.
  15. Peix A, Ramirez-Bahena MH, and Velazquez E. 2009. Historical evolution and current status of the taxonomy of genus Pseudomonas. Infect. Genet. Evol. 9, 1132-1147.
  16. Saitou N, and Nei M. 1987. The neighbor-joining method-a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  17. Schadler S, Burkhardt C, and Kappler A. 2008. Evaluation of electron microscopic sample preparation methods and imaging techniques for characterization of cell-mineral aggregates. Geomicrobiol. J. 25, 228-239.
  18. Stanier RY, Palleroni NJ, and Doudoroff M. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43, 159-271.
  19. Tambong JT, Xu R, and Bromfield ESP. 2017. Pseudomonas canadensis sp. nov., a biological control agent isolated from a field plot under long-term mineral fertilization. Int. J. Syst. Evol. Microbiol. 67, 889-895.
  20. Tamura K, Stecher G, Peterson D, Filipski A, and Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729.
  21. Verhille S, Baida N, Dabboussi F, Izard D, and Leclerc H. 1999. Taxonomic study of bacteria isolated from natural mineral waters: Proposal of Pseudomonas jessenii sp. nov. and Pseudomonas mandelii sp. nov. Syst. Appl. Microbiol. 22, 45-58.
  22. Weisburg WG, Barns SM, Pelletier DA, and Lane DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697-703.