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Isolation and characterization of 6 unrecorded Pseudomonas spp. from Korean soil

토양에서 분리한 국내 미기록종 Pseudomonas 속 6종의 생화학적 특성과 계통 분류

  • Kim, Hyun-Joong (Institute of Life Sciences and Resources Graduate School of Biotechnology, Kyung Hee University) ;
  • Jung, You-Jung (Biological Resources Research Department, National Institute of Biological Resources) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources Graduate School of Biotechnology, Kyung Hee University) ;
  • Hur, Moonsuk (Biological Resources Research Department, National Institute of Biological Resources)
  • 김현중 (경희대학교 생명과학대학 식품생명공학 전공) ;
  • 정유정 (국립생물자원관 생물자원연구부 미생물자원과) ;
  • 김해영 (경희대학교 생명과학대학 식품생명공학 전공) ;
  • 허문석 (국립생물자원관 생물자원연구부 미생물자원과)
  • Received : 2018.11.30
  • Accepted : 2018.12.19
  • Published : 2019.03.31

Abstract

In 2017, as a study to discover indigenous prokaryotic species in Korea, a total of 6 bacterial strains assigned to the genus Pseudomonas were isolated from soil. From the high 16S rRNA gene sequence similarity (${\geq}99.5%$) and phylogenetic analysis with closely related species, the isolated strains were identified as independent Pseudomonas species which were unrecorded in Korea. The six Pseudomonas species were Pseudomonas mandelii, P. canadensis, P. thivervalensis, P. jessenii, P. lurida, and P. brenneri. Gram reaction, culture conditions, colony and cell morphology, basic physiological and biochemical characteristics are described in the species description section.

Keywords

Pseudomonas;16S rRNA gene sequencing;gammaproteobacteria;soil;unrecorded species

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Fig. 1. Phylogenetic tree of Pseudomonas spp. based on the 16S rRNA gene sequence analysis using the neighbor-joining method showing the position of the newly isolated Pseudomonas strains (highlighted in bold) compared with the closely reference Pseudomonas strains.

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Fig. 2. Transmission electron micrograph of isolated Pseudomonas spp.

Table 1. Pseudomonas strains isolated from soil in this study

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Table 2. Physiological and biochemical characteristics and differential carbohydrate assimilation patterns of isolated Pseudomonas spp. using API 20NE (2.1) kit, API ZYM kit, and API 32GN kit

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Acknowledgement

Supported by : 국립생물자원관

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