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Removal of mycotoxin ochratoxin A by isolated bacteria

분리세균에 의한 진균독소 ochratoxin A의 제거

  • Choi, Ho-Yeong (Department of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • 최호영 (강원대학교 생명과학과) ;
  • 송홍규 (강원대학교 생명과학과)
  • Received : 2018.11.19
  • Accepted : 2019.01.03
  • Published : 2019.03.31

Abstract

Ochratoxin A (OTA), one of mycotoxins produced mainly by Aspergillus is a common contaminant of stored grains, posing health hazards to human and livestock. The aim of this study is to explore ability of isolated bacteria Bacillus subtilis AF13 and Streptomyces shenzhenensis YR226 to remove OTA. AF13 and YR226 could remove 94.23 and 97.73% of OTA ($100{\mu}g/L$), respectively during 24 h incubation in NB medium. When cultures of two strains were separated into washed cells and cell-free supernatant, the supernatant of both strains removed more than 90% of $100{\mu}g/L$ OTA, and 98.88% of OTA could be also removed by the washed cells of YR226. OTA removal occurred in a few second by the supernatant of both strains, and treatments of autoclaving, proteinase K and chymotrypsin did not affect the OTA removal by the culture supernatants, which indicate that some thermostable and non-proteinaceous substances secreted by these bacteria may be involved in OTA removal in these two bacteria. These results suggest that AF13 and YR226 can be used to remove OTA from OTA-contaminated grains and feeds, and therefore decrease economic damage in agriculture and feed industry.

Keywords

Bacillus subtilis AF13;Streptomyces shenzhenensis YR226;mycotoxin;ochratoxin A (OTA);OTA removal

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Fig. 1. OTA (100 μg/L) removal by culture supernatant (■) and washed cells (□) of AF13 and YR226 in phosphate buffer (37℃, 150 rpm, 24 h). ***, significant at P < 0.001.

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Fig. 2. Time course of OTA (100 μg/L) removal by culture supernatant of AF13 (○) and YR226 (◇) at 37℃.

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Fig. 3. Time course of OTA (100 μg/L) removal by washed cells of YR226 (◇) (37℃, 150 rpm, 72 h). **, significant at P < 0.01; ***, significant at P < 0.001.

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Fig. 4. Effect of temperature on OTA (100 μg/L) removal in culture of AF13 (○) and YR226 (◇) (150 rpm, 24 h).

Table 1. Effect of treatment of heat, proteinase K (1 mg/ml) and chymotrypsin (1 mg/ml) on OTA (100 µg/L) removal by culture supernatant of strains AF13 and YR226

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Acknowledgement

Supported by : 중소기업청

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