Removal of mycotoxin ochratoxin A by isolated bacteria

분리세균에 의한 진균독소 ochratoxin A의 제거

  • Choi, Ho-Yeong (Department of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Department of Biological Sciences, Kangwon National University)
  • 최호영 (강원대학교 생명과학과) ;
  • 송홍규 (강원대학교 생명과학과)
  • Received : 2018.11.19
  • Accepted : 2019.01.03
  • Published : 2019.03.31


Ochratoxin A (OTA), one of mycotoxins produced mainly by Aspergillus is a common contaminant of stored grains, posing health hazards to human and livestock. The aim of this study is to explore ability of isolated bacteria Bacillus subtilis AF13 and Streptomyces shenzhenensis YR226 to remove OTA. AF13 and YR226 could remove 94.23 and 97.73% of OTA ($100{\mu}g/L$), respectively during 24 h incubation in NB medium. When cultures of two strains were separated into washed cells and cell-free supernatant, the supernatant of both strains removed more than 90% of $100{\mu}g/L$ OTA, and 98.88% of OTA could be also removed by the washed cells of YR226. OTA removal occurred in a few second by the supernatant of both strains, and treatments of autoclaving, proteinase K and chymotrypsin did not affect the OTA removal by the culture supernatants, which indicate that some thermostable and non-proteinaceous substances secreted by these bacteria may be involved in OTA removal in these two bacteria. These results suggest that AF13 and YR226 can be used to remove OTA from OTA-contaminated grains and feeds, and therefore decrease economic damage in agriculture and feed industry.

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Fig. 1. OTA (100 μg/L) removal by culture supernatant (■) and washed cells (□) of AF13 and YR226 in phosphate buffer (37℃, 150 rpm, 24 h). ***, significant at P < 0.001.

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Fig. 2. Time course of OTA (100 μg/L) removal by culture supernatant of AF13 (○) and YR226 (◇) at 37℃.

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Fig. 3. Time course of OTA (100 μg/L) removal by washed cells of YR226 (◇) (37℃, 150 rpm, 72 h). **, significant at P < 0.01; ***, significant at P < 0.001.

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Fig. 4. Effect of temperature on OTA (100 μg/L) removal in culture of AF13 (○) and YR226 (◇) (150 rpm, 24 h).

Table 1. Effect of treatment of heat, proteinase K (1 mg/ml) and chymotrypsin (1 mg/ml) on OTA (100 µg/L) removal by culture supernatant of strains AF13 and YR226

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Supported by : 중소기업청


  1. Abrunhosa L, Ines A, Rodrigues A, Guimaraes A, Pereira V, Parpot P, Mendes-Raia A, and Venancio A. 2014. Biodegradation of ochratoxin A by Pediococcus parvulus isolated from Douro wines. Int. J. Food Microbiol. 188, 45-52.
  2. Bellis PD, Tristezza M, Haidukowski M, Fanelli F, Sisto A, Mule G, and Grieco F. 2015. Biodegradation of ochratoxin A by bacterial strains isolated from vineyard soils. Toxins 7, 5079-5093.
  3. Birzele B, Prange A, and Kramer J. 2000. Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters. Food Addit. Contam. 17, 1027-1035.
  4. Bosco F and Mollea C. 2012. Mycotoxins in food, pp. 169-200. In Valdez B. (ed.), Food industrial processes - Methods and equipment. InTech, Rijeka, Croatia.
  5. Fazekas B, Tar AK, and Zomborszky-Kovacs M. 2002. Ochratoxin A contamination of cereal grains and coffee in Hungary in the year 2001. Acta Vet. Hung. 50, 177-188.
  6. Ferenczi S, Cserhati M, Krifaton C, Szoboszlay S, Kukolya J, Szoke Z, Koszegi B, Albert M, Barna T, Mezes M, et al. 2014. A new ochratoxin a biodegradation strategy using Cupriavidus basilensis strain. PLoS One 9, e109817.
  7. Hasheminya SM and Dehghannya J. 2013. Strategies for decreasing aflatoxin in livestock feed and milk. Int. Res. J. Appl. Basic Sci. 4, 1506-1510.
  8. Hwang JH and Lee KG. 2006. Reduction of aflatoxin $B_1$ contamination in wheat by various cooking treatments. Food Chem. 98, 71-75.
  9. International Agency for Research on Cancer (IARC). 1993. Some naturally occurring substances: Food items and consituents, heterocyclic aromatic amines and mycotoxins, pp. 490-523. In IARC working group on the evaluation of carcinogenic risks to humans (ed.), IARC monographs on the evaluation of carcinogenic risks to humans, No. 56. Lyon, France.
  10. Korea Ministry of Food and Drug Safety (KMFDS). 2017. Common standards and specification for general food, p. 41. In Food and drug administration (ed.), Food Code (No. 2017-57_20170630), Korea.
  11. Li S, Marquardt RR, Frohlich AA, Vitti TG, and Crow G. 1997. Pharmacokinetics of ochratoxin A and its metabolites in rats. Toxicol. Appl. Pharmacol. 145, 82-90.
  12. Mishra HN and Das C. 2003. A review on biological control and metabolism of aflatoxin. Crit. Rev. Food Sci. Nutr. 43, 245-264.
  13. Mitchinson C and Pain RH. 1985. Effects of sulphate and urea on the stability and reversible unfolding of ${\beta}$-lactamase from Staphylococcus aureus implications for the folding pathway of ${\beta}$-lactamase. J. Mol. Biol. 184, 331-342.
  14. Petruzzi L, Bevilacqua A, Baiano A, Beneduce L, Corbo MR, and Sinigaglia M. 2013. In vitro removal of ochratoxin A by two strains of Saccharomyces cerevisiae and their performances under fermentative and stressing conditions. J. Appl. Microbiol. 116, 60-70.
  15. Pitout MJ. 1969. The hydrolysis of ochratoxin A by some proteolytic enzymes. Biochem. Pharmacol. 18, 485-491.
  16. Qi DZ, Guo HL, Wang DB, Yi S, Jing GL, Zheng HX, and Yong NW. 2014. Exposure assessment to ochratoxin A in Chinese wine. J. Agric. Food Chem. 62, 8908-8913.
  17. Roussos S, Zaouia N, Salih G, Tantaoui-Elaraki A, Lamrani K, Cheheb M, Hassouni H, Verhe F, Gaime I, and Augur C. 2006. Characterization of filamentous fungi isolated from Moroccan olive and olive cake: Toxinogenic potential of Aspergillus strains. Mol. Nutr. Food Res. 50, 500-506.
  18. Shi L, Liang Z, Li J, Hao J, Xu Y, Huang K, Tian J, Hea X, and Xu W. 2014. Ochratoxin A biocontrol and biodegradation by Bacillus subtilis CW 14. J. Sci. Food Agric. 94, 1879-1885.
  19. Upadhaya S, Song J, Oark M, Seo J, Yang L, Lee C, Cho K, and Ha J. 2012. Isolation, screening and identification of swine gut microbiota with ochratoxin A biodegradation ability. Asian-Aus. J. Anim. Sci. 25, 114-121.
  20. van der Merwe KJ, Steyn PS, and Fourie L. 1965. 1304. Mycotoxins. Part II. The constitution of ochratoxins A, B, and C, metabolites of Aspergillus ochraceus Wilh. J. Chem. Soc. 7083-7088.
  21. Wang C, Li Z, Wang H, Qiu H, Zhang M, Li S, Luo X, Song Y, Zhou H, Ma W, et al. 2017. Rapid biodegradation of aflatoxin $B_1$ by metabolites of Fusarium sp. WCQ3361 with broad working temperature range and excellent thermostability. J. Sci. Food Agric. 97, 1342-1348.
  22. Wangikar PB, Dwivedi P, Sinha N, Sharma AK, and Telang AG. 2005. Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicology 215, 37-47.
  23. Xiong K, Wang X, Zhi HW, Suna BG, and Lia XT. 2016. Identification and safety evaluation of a product from the biodegradation of ochratoxin A by an Aspergillus strain. J. Sci. Food Agric. 97, 434-443.
  24. Zhang H, Wang Y, Zhao C, Wang J, and Zhang X. 2017. Biodegradation of ochratoxin A by Alcaligenes faecalis isolated from soil. J. Appl. Microbiol. 123, 661-668.
  25. Zhou T, He J, and Gong J. 2008. Microbial transformation of trichothecene mycotoxins. World Mycotoxin J. 1, 23-30.