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Post-pandemic influenza A (H1N1) virus detection by real-time PCR and virus isolation

  • Zaki, Ali Mohamed (Medical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University) ;
  • Taha, Shereen El-Sayed (Medical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University) ;
  • Shady, Nancy Mohamed Abu (Pediatrics Department, Faculty of Medicine, Ain Shams University) ;
  • Abdel-Rehim, Asmaa Saber (Internal Medicine Department, Faculty of Medicine, Ain Shams University) ;
  • Mohammed, Hedya Said (Chest Diseases Department, Faculty of Medicine, Ain Shams University)
  • Received : 2018.12.17
  • Accepted : 2018.12.28
  • Published : 2019.03.31

Abstract

Influenza A (H1N1) virus caused a worldwide pandemic in 2009-2010 and still remains in seasonal circulation. Continuous surveillance activities are encouraged in the post pandemic phase to watch over the trend of occurrence every year, this is better to be done by a rapid and sensitive method for its detection. This study was conducted to detect proportions of occurrence of influenza A virus (H1N1) in patients with influenza-like illness. Samples from 500 patients with influenza or influenza-like clinical presentation were tested by real-time reverse transcription polymerase chain reaction (RT-PCR) and virus tissue culture. Among the total 500 participants, 193 (38.6%) were females and 307 (61.4%) males. Seventy-one patients (14.2%) were positive for H1N1 virus infection with real-time RT-PCR while 52 (10.4%) were positive by tissue culture. Non-statistically significant relation was found between age and gender with the positivity of H1N1. Sensitivity and specificity of real-time RT-PCR was 98.08% and 95.54%, respectively, in comparison to virus isolation with accuracy 95.8%. This study showed that H1N1 virus was responsible for a good proportion of influenza during the post-pandemic period. Real-time RT-PCR provides rapidity and sensitivity for the detection of influenza A virus (H1N1) compared with virus isolation and thus it is recommended as a diagnostic tool.

Keywords

influenza A virus;H1N1;post pandemic;real-time RT-PCR and tissue culture;seasonal influenza

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Fig. 1. Amplification plot of H1N1 positive samples.

Table 1. Relation between of influenza A (H1N1) infection and gender

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Table 2. Distribution of influenza A (H1N1) infection by age group

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Table 3. Relation between of influenza A (H1N1) infection and age

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Table 4. Clinical manifestations of influenza in the infected population

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Table 7. The performance of real time RT-PCR in relation to conventional viral culture

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Table 5. Comorbid conditions in the infected population

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Table 6. Seasonal distribution of influenza A (H1N1) virus infection

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