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Novel insight into the role of thiamine for the growth of a lichen-associated Arctic bacterium, Sphingomonas sp., in the light

Sphingomonas 속 세균의 명조건 생장에서 티아민의 필수적인 역할

  • Pham, Nhung (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University) ;
  • Pham, Khoi (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University) ;
  • Lee, ChangWoo (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University) ;
  • Jang, Sei-Heon (Department of Biomedical Science and Center for Bio-Nanomaterials, Daegu University)
  • 팜눙 (대구대학교 의생명과학과) ;
  • 팜코이 (대구대학교 의생명과학과) ;
  • 이창우 (대구대학교 의생명과학과) ;
  • 장세헌 (대구대학교 의생명과학과)
  • Received : 2019.01.28
  • Accepted : 2019.03.14
  • Published : 2019.03.31

Abstract

Bacteria in the polar region are under strong light and ultraviolet radiation. In this study, we investigated the effects of light on the growth of a psychrophilic bacterium, Sphingomonas sp. PAMC 26621, isolated from an Arctic lichen Cetraria sp. The growth of the strain in the light was lower than that in the dark. Surprisingly, thiamine increased the growth of Sphingomonas sp. PAMC 26621 in M9 minimal medium under light conditions. Thiamine increased the growth of the strain in a concentration-dependent manner along with ascorbic acid. N-acetylcysteine had no effect on the growth of the strain in the light. Thiamine and ascorbic acid also increased the activities of glucose-6-phosphate dehydrogenase and superoxide dismutase. The results of this study indicate that thiamine provided by the lichen symbiosis system plays an important role in light-induced oxidative stress in this Arctic bacterium as an antioxidant. Our study provide insight into the biochemistry and physiology of Arctic bacteria under strong light and ultraviolet radiation.

Keywords

Sphingomonas sp. PAMC 26621;ascorbic acid;light;NADPH;thiamine

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Fig. 1. The growth of Sphingomonas sp. PAMC 26621 in M9 medium and MT medium under light condition (A) and dark condition (B).

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Fig. 2. The effects of antioxidants on the growth of Sphingomonas sp. PAMC 26621.

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Fig. 3. Activities of G6PDH at mid-log and stationary phase on MT medium, M9 + thiamine, M9 + ascorbic acid, M9 (light), M9 (dark) (A).

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Fig. 4. Native polyacrylamide gel stained for SOD activity of samples from Sphingomonas sp. PAMC 26621.

Acknowledgement

Supported by : Daegu University

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