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A highly efficient computational discrimination among Streptococcal species of periodontitis patients using 16S rRNA amplicons

  • Al-Dabbagh, Nebras N. (Department of Microbiology, College of Dentistry, University of Babylon) ;
  • Hashim, Hayder O. (Department of Clinical Laboratory Sciences, College of Pharmacy, University of Babylon) ;
  • Al-Shuhaib, Mohammed Baqur S. (Department of Animal Production, College of Agriculture, Al-Qasim Green University)
  • Received : 2018.12.17
  • Accepted : 2019.03.15
  • Published : 2019.03.31

Abstract

Due to the major role played by several species of Streptococcus in the etiology of periodontitis, it is important to assess the pattern of Streptococcus pathogenic pathways within the infected subgingival pockets using a bacterial specific 16S rRNA fragment. From the total of 50 patients with periodontitis included in the study, only 23 Streptococcal isolates were considered for further analyses, in which their 16S rRNA fragments were amplified and sequenced. Then, a comprehensive phylogenetic tree was constructed and in silico prediction was performed for the observed Streptococcal species. The phylogenetic analysis of the subgingival Streptococcal species revealed a high discrimination power of the 16S rRNA fragment to accurately identify three groups of Streptococcus on the species level, including S. salivarius (14 isolates), S. anginosus (5 isolates), and S. gordonii (4 isolates). The employment of state-of-art in silico tools indicated that each Streptococcal species group was characterized with particular transcription factors that bound exclusively with a different 16S rRNA-based secondary structure. In conclusion, the observed data of the present study provided in-depth insights into the mechanism of each Streptococcal species in its pathogenesis, which differ in each observed group, according to the differences in the 16S rRNA secondary structure it takes, and the consequent binding with its corresponding transcription factors. This study paves the way for further interventions of the in silico prediction, with the main conventional in vitro microbiota identification to present an interesting insight in terms of the gene expression pattern and the signaling pathway that each pathogenic species follows in the infected subgingival site.

Keywords

Streptococcus anginosus;Streptococcus gordonii;Streptococcus salivarius;in silico;periodontitis

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Fig. 1. The comprehensive phylogenetic tree of the 1,492 bp variants of the 16S rRNA gene fragment for Streptococcal stains that isolated from subgingival sites of periodontitis patients.

MSMHBQ_2019_v55n1_1_f0002.png 이미지

Fig. 2. Putative two-dimensional secondary structures of three16S rRNA variants of three Streptococcal species isolated from subgingival sites of periodontitis patients.

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