Fig. 1. HPLC chromatogram of extract of soybean germ. (A) Daidzin (B) Genistin (C) Daidzein (D) Genistein
Fig. 2. Amount of isoflavone isolated from Soybean germ.
Fig. 3. Bioconversioin yield of isoflavones extracted from soybean germ using Pectinase.
Fig. 4. Effect of bioconversioned soybean germ extract on cell viability. Result is resented as mean ± SD. (A) RAW 264.7 cells (B) B16F10 cells (C) HDF cells.
Fig. 5. Inhibitory effect of bioconversioned soybean germ extract on LPS-induced NO production by RAW 264.7 cells. Result is represented as mean ± SD. *p<0.05
Fig. 6. Inhibition of melanin synthesis in B16F10 melanoma cells treated with bioconversioned soybean germ extract. Result is represented as mean ± SD. *p<0.05 **p<0.01 ***p<0.001
Fig. 7. Inhibitory effects of bioconversioned soybean germ extract on UVB-induced MMP-1 in HDF cells. Result is represented as mean ± SD. **p<0.01 ***p<0.001
Table 1. Gradient condition for HPLC analysis
Table 2. Formulation of wash-off pack containing bioconversioned soybean germ extract
Table 3. Irritant classification criteria and skin irritation score system (A) Irritant classification criteria (B) skin rritation score systemA
Table 4. Changes in roughness of skin.
Table 5. Changes in roughness of skin.