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Physiological activity and efficacy of cosmetic products in bio-converted soybean embryo extract

생물전환 콩 배아추출물의 생리활성 및 화장품의 효능 연구

  • Min, Kyung-Nam (Department of Cosmetics inventive Design, Anyang University) ;
  • Lee, Gun-Hee (Department of Microbio Engineering, Konkuk University) ;
  • Park, Sung-Jin (Department of Food and Biotechnology, Korea University) ;
  • Choe, Tae-Boo (Department of Cosmetics Engineering, Konkuk University)
  • 민경남 (안양대학교 화장품발명디자인학과) ;
  • 이건희 (건국대학교 생물공학과) ;
  • 박성진 (고려대학교 식품생명공학과) ;
  • 최태부 (건국대학교 화장품공학과)
  • Received : 2018.09.18
  • Accepted : 2019.03.20
  • Published : 2019.03.28

Abstract

This study was to investigate the physiological activities of bioconversion soybean embryo extract and the effects of sleeping pack containing extract on skin. The aglycone isoflavones of the soybean embryo extract bioconversed with pectinase were identified by HPLC. in vitro tests showed that they were non-toxic, anti-inflammatory, anti-melanin and anti-aging activity. After 4 weeks of human body application with the sleeping pack using extract, the skin surface roughness and eye wrinkles were so improved by low stimulation that the sleeping pack containing bioconversion soybean embryo extract has a positive effect on the skin. We confirmed that the bioconversion soybean embryo extract was effective in enhancing the physiological activity and was useful as a cosmetic material of various formulations.

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Fig. 1. HPLC chromatogram of extract of soybean germ. (A) Daidzin (B) Genistin (C) Daidzein (D) Genistein

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Fig. 2. Amount of isoflavone isolated from Soybean germ.

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Fig. 3. Bioconversioin yield of isoflavones extracted from soybean germ using Pectinase.

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Fig. 4. Effect of bioconversioned soybean germ extract on cell viability. Result is resented as mean ± SD. (A) RAW 264.7 cells (B) B16F10 cells (C) HDF cells.

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Fig. 5. Inhibitory effect of bioconversioned soybean germ extract on LPS-induced NO production by RAW 264.7 cells. Result is represented as mean ± SD. *p<0.05

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Fig. 6. Inhibition of melanin synthesis in B16F10 melanoma cells treated with bioconversioned soybean germ extract. Result is represented as mean ± SD. *p<0.05 **p<0.01 ***p<0.001

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Fig. 7. Inhibitory effects of bioconversioned soybean germ extract on UVB-induced MMP-1 in HDF cells. Result is represented as mean ± SD. **p<0.01 ***p<0.001

Table 1. Gradient condition for HPLC analysis

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Table 2. Formulation of wash-off pack containing bioconversioned soybean germ extract

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Table 3. Irritant classification criteria and skin irritation score system (A) Irritant classification criteria (B) skin rritation score systemA

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Table 4. Changes in roughness of skin.

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Table 5. Changes in roughness of skin.

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