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Effects of a Glycoprotein Isolated from Ulmus davidiana Nakai on Toluene-Induced Ecotoxicity and its Mechanism in Human Intestinal Epithelial Cells

소장상피세포에 있어서 느릅나무 당단백질이 톨루엔에 의해 유도된 환경독성 기작에 미치는 효과

  • Kim, Do-Wan (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Kim, Ji-Yun (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Park, Moon-Ki (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Lee, Sei-Jung (Department of Pharmaceutical Engineering, Daegu Haany University)
  • 김도완 (대구한의대학교 제약공학과) ;
  • 김지윤 (대구한의대학교 제약공학과) ;
  • 박문기 (대구한의대학교 제약공학과) ;
  • 이세중 (대구한의대학교 제약공학과)
  • Received : 2019.01.18
  • Accepted : 2019.02.12
  • Published : 2019.02.28

Abstract

Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine to treat inflammatory diseases in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprotein) in human intestinal epithelial INT-407 cells. We demonstrated that UDN glycoprotein ($20{\mu}g/mL$) could inhibit the production of lactate dehydrogenase (LDH) induced by toluene, an ecotoxic substance. Additionally, we found that the toluene-induced intestinal cytotoxicity was mediated by the phosphorylation of p38 Mitogen-Activated Protein Kinase (MAPK) via the production of intracellular Reactive Oxygen Species (ROS). The UDN glycoprotein significantly decreased the levels of ROS production and p38 MAPK activation in toluene-stimulated INT-407 cells. Moreover, the UDN glycoprotein inhibits the phosphorylation of nuclear factor-kappa B ($NF-{\kappa}B$), which is responsible for the production of LDH, in toluene-stimulated INT-407 cells. Collectively, our data indicate that UDN glycoprotein is a natural antioxidant and a modulator of ecotoxicity signaling pathways in human intestinal epithelial cells.

Keywords

Ulmus davidiana Nakai (UDN) glycoprotein;Intestinal epithelial cells;Reactive oxygen species;$NF-{\kappa}B$;Toluene

Acknowledgement

Supported by : 경산시

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