DOI QR코드

DOI QR Code

Cloning and Sequencing of the Mitochondrial Cytochrome c Oxidase Subunit II Gene from Rhabditidae Family Nematode

Rhabditidae과 선충의 CO II 유전자 클로닝 및 염기서열 분석

  • Lee, Sang Mong (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Son, Hong Joo (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Kim, Keun Ki (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Hong, Chang Oh (Department of Life Science & Environmental Biochemistry, Pusan National University) ;
  • Park, Hyean Cheal (Department of Life Science & Environmental Biochemistry, Pusan National University)
  • 이상몽 (부산대학교 생명환경화학과) ;
  • 손홍주 (부산대학교 생명환경화학과) ;
  • 김근기 (부산대학교 생명환경화학과) ;
  • 홍창오 (부산대학교 생명환경화학과) ;
  • 박현철 (부산대학교 생명환경화학과)
  • Received : 2018.10.31
  • Accepted : 2018.12.13
  • Published : 2019.01.31

Abstract

Cytochrome c oxidase subunit II gene(CO II gene) is subunit of cytochrome oxidase, which is complex IV of mitochondria electron transport system. It has been frequently used in molecular phylogenetic studies because the speed of its DNA variation is faster than that of nucleus. It is especially useful in phylogenetic study of molecular biology in insects. In this study, we cloned and sequenced CO II gene of mitochondria DNA from Rhabditidae family nematode. Our results showed that this gene is comprised of 696 base pairs(bp). In the analysis of similarity of this gene with other known genes of 14 species of nematodes in Rhabditida order, we identified that this gene has high similarity with that of Caenorhabditis briggsae(86.0%) and C. elegans(85.6%) in Rhabditidae family. On the meanwhile, it has very low similarity with that of Angiostrongylus cantonensis(31.8%) in Angiostrongylidae family and Metastrongylus salmi(31.6%) in Metastrongylidae family. Based on the results of this study, we suggest that this nematode is closely related with that of Caenorhabditis genus in Rhabditidae family.

Acknowledgement

Supported by : 부산대학교

References

  1. Agrios, G. N., 2005, Plant pathology, 5th ed., Academic Press, New York, 922.
  2. Andrassy, I., 1976, Evolution as a basis for the systematization of nematodes, 1st ed., Pitman, London, 288.
  3. Avise, J. C., 1994, Molecular markers, natural history, and evolution, 1st ed., Chapman & Hall, New York, 511.
  4. Avise, J. C., 1995, Mitochondrial DNA polymorphism and a connection between genetics and demography of relevance to conservation, Cons. Biol., 9, 686-690. https://doi.org/10.1046/j.1523-1739.1995.09030686.x
  5. Chitwood, B. G., Chitwood, M. B., 1974, Introduction to nematology, University Park Press, Baltimore, London, Tokyo, 334.
  6. Dougherty, E. C., 1955, The genera and species of subfamily Rhabditinae Micoletzky, 1922 (Nematoda): a nomenclatorial analysis -including an addendum on the composition of the family Rhabditidae Orley, 1880, J. Helminthol., 29(3), 105-l52. https://doi.org/10.1017/S0022149X00024317
  7. Liu, H., Beckenbach, A. T., 1992, Evolution of the mitochondrial cytochrome oxidase gene among 10 orders of insects, Mol. Phylog. Evol., 1(1), 41-52. https://doi.org/10.1016/1055-7903(92)90034-E
  8. Maggenti, A., 1981, General nematology, Springer, New York, 1-3.
  9. McCarter, J. P., 2009, Molecular approaches toward resistance to plant-parasitic nematodes, in: Berg, R. H., Taylor, C. G. (eds.), Cell biology of plant nematode parasitism, Vol. 15, Springer-Verlag Press, Berlin, 239-267.
  10. Micoletzky, H., 1925, Die freilebenden susswasser-und moornematoden Danemarks nebst anhanguber amobosporidien und andere parasiten bei freilebenden Nematoden, Kong. Dans. Viden. Sel. Skr., 10(2), 55-310.
  11. Miura, T., Roisin, Y., Matusmoto, T., 2000, Molecular phylogeny and biogeography of the nasute termite genus Nasutitermes (Isoptera: Termitidae) in the Pacific tropics, Mol. Phylogenet. Evol., 17(1), 1-10. https://doi.org/10.1006/mpev.2000.0790
  12. Nicol, J. M., Turner, S. J., Coyne, D. L., den Nijs, L., Hockland, S., Maafi, Z. T., 2011, Current nematode threats to world agriculture, in: Jones, J. T., Gheysen, G., Fenoll, C. (eds.), Genomics and molecular genetics of plant nematode interactions, Springer, Heidelberg, 21-44.
  13. Osche, G., 1952, Systematik und phylogenie der Gattung rhabditis (Nematoda), Zool. Jahab. Abt. Syst., 81, 190-280.
  14. Sudhaus, W., 1976, Vergleichende untersuchungen zur phylogenie, systematik, okologie, biologie und ethologie der Rhabditidae (Nematoda), Zoologica, 43, l-229.
  15. Sudhaus, W., 1991, Check list of species of Rhabditis sensu lato (Neiatoda: Rhabditidae) discovered between 1976 and 1986, Nematologica, 37, 229-236. https://doi.org/10.1163/187529291X00213
  16. Winslow, R. D., 1960, Some aspects of the ecology of free-living and plant-parasitic nematodes, in: Sasser, J. N., Jenkins, W. R. (eds), Nematology: fundamentals and recent advances with emphasis on plant parasitic and soil forms, University of North Carolina Press, Chapel Hill, 341-415.
  17. Yeates, G. W., Bongers, T., de Goede, R. G. M., Freckman, D. W., Georgieva, S. S., 1993, Feeding habits in soil nematode families and genera - an outline for soil ecologists, J. Nematol., 25, 101-313.