Microbial changes under packaging conditions during transport and comparison between sampling methods of beef

  • Yim, Dong-Gyun (Department of Animal Science, Sangji University) ;
  • Jin, Sang-Keun (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Hur, Sun-Jin (Department of Animal Science and Technology, Chung-Ang University)
  • Received : 2019.01.07
  • Accepted : 2019.01.11
  • Published : 2019.01.31


This study was performed to evaluate the microbial and temperature changes of boxed beef during transport and distribution under vacuum and modified atmosphere packaging (MAP), and to compare between excision and swab sampling for 15 days. The top round and striploin (quality grade 1) from Hanwoo steers at 2 days post-slaughter were obtained from a local meat processing plants and chilled at $4{\pm}2^{\circ}C$ in a cold room. The boxes were transported under refrigeration ($4{\pm}2^{\circ}C$) to the laboratory within half an hour. Vacuum and MAP packs were subsequently taken out from cool boxes, and microbiological examinations were carried out at 0, 6, 12, and 24 h of storage time. MAP was more effective than vacuum packaging for the inhibition of total aerobic, lactic acid bacteria and Pseudomonas (p < 0.05). Microbial loads of swab methods were slightly lower than those of excision ones (p < 0.05). The results of this study could be utilized by meat consumers in future studies as well as by manufacturers to determine the ideal storage conditions for cool boxed meat, thus ensuring reduced economic losses due to spoilage.


Cool boxed beef;Microorganism;Packaging method


Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET)


  1. Taoukis P, Labuza TP. Time-temperature indicators (TTIs). In: Ahvenainen R, editor. Novel food packaging techniques. Cambridge, UK: Woodhead Publishing; 2003. p. 103-26.
  2. Koutsoumanis K, Stamatiou A, Skandamis P, Nychas GJE. Development of a microbial model for the combined effect of temperature and pH on spoilage of ground meat, and validation of the model under dynamic temperature conditions. Appl Environ Microbiol. 2006;72:124-34.
  3. Farris DE, Dietrich RA, Ward JB. Reducing the cost of marketing beef. Beef prices increase need for central packaging. Meat Processing. 1991;30:60-2.
  4. Pearson AM. Introduction to quality attributes and their measurements in meat, poultry and fish products. In Pearson AM, Dutson TR, editors. Advances in meat research. London: Blackie Academic; 1994;9:1-17.
  5. Gill CO, McGinnis JC, Rahn K, Houde A. Control of product temperatures during the storage and transport of bulk containers of manufacturing beef. Food Res Int. 1996;29:647-51.
  6. Brody AL. Meat packaging: past, present and future. 55th reciprocal meat conference; East Lansing, Michigan, USA. 2002.
  7. McMillin KW. Where is MAP going? A review and future potential of modified atmosphere packaging for meat. Meat Sci. 2008;80:43-65.
  8. Bingol EB, Ergun O. Effects of modified atmosphere packaging (MAP) on the microbiological quality and shelf life of ostrich meat. Meat Sci. 2011;88:774-85.
  9. Martinez B, Celda MF, Millan ME, Espacio A, Cano M, Lopez-Mendoza MC. Assessment of the microbiological conditions of red-meat carcasses from bacterial counts recovered by sampling via excision or swabbing with cotton wool. Int J Food Sci Technol. 2009;44:770-6.
  10. Belcher JN. Industrial packaging developments for the global meat market. Meat Sci. 2006;74:143-8.
  11. Palumbo SA, Klein P, Capra J, Eblen S, Millear AJ. Comparison of excision and swabbing sampling methods to determine the microbiological quality of swine carcass surfaces. Food Microbiol. 1999;16:459-64.
  12. Hutchison ML, Walters LD, Avbery SM, Reid CA, Wilson D, Howell M, et al. A comparison of wet-dry swabbing and excision sampling methods for microbiological testing of bovine, porcine, and ovine carcasses at red meat slaughterhouses. J Food Protec. 2005;68:2155-62.
  13. Capita R, Prieto M, Alonso-calleja C. Sampling methods for microbiological analysis of red meat and poultry carcasses. J Food Protec. 2004;67:1303-8.
  14. MFDS [Ministry of Food and Drug Safety]. Korean food standards codex (No. 2011-76) No. 10. General method. 2015. Report No.: 10-3-35.
  15. QIA [Animal and Plant Quarantine Agency]. Standards for processing and ingredients specifications of livestock products, animal, plant and fisheries quarantine and inspection agency notification (No. 2012-118). Korea: Animal, Plant and Fisheries Quarantine and Inspection Agency; 2016.
  16. SPSS. PASW statistics 21. Illinois, USA: Statistical Package for the Social Sciences Incorporated; 2011.
  17. Chouliara E, Karatapanis A, Savvaidis IN, Kontominas MG. Combined effect of oregano essential oil and modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at $4^{\circ}C$. Food Microbiol. 2007;24:607-17.
  18. Chung KY, Chung ER, Lee HJ. Quality changes of Supraspinatus M. of Hanwoo by packaging methods during chilled storage. Korean J Food Sci Anim Resour. 2007;27:469-74.
  19. Gill CO. 1996. Extending the storage life of raw chilled meats. Meat Sci. 19961;43 Suppl 1:99-109.
  20. Kennedy C, Buckley DJ, Kerry JP. Display life of sheep meats retail packaged under atmospheres of various volumes and compositions. Meat Sci. 2004;68:649-58.
  21. Argyri AA, Doulgeraki AI, Blana VA, Panagou EZ, Nychas GJE. Potential of a simple HPLC based approach for the identification of the to quantify spoilage status of minced beef stored at different various temperatures and packaging systems. Int J Food Microbiol. 2011;150:25-33.
  22. Dainty RH, Mackey BM. The relationship between the phenotypic properties of bacteria from chill-stored meat and spoilage processes. J Applied Bacteriol Symp. 1992;73 Suppl: 103S-14S.
  23. Yang YM, Son JW, Choi TS, Park MA, Kim JY, Lee JH, et al. A survey of the microbial contamination level in butcher’s shops in Seoul, Korea. Korean J Vet Serv. 2013;36:203-8.
  24. Ko EK, Heo EJ, Kim YJ, Park HJ, Wiee SH, Moon JS. Evaluation on microbiological contamination level of raw beef from retail markets in Seoul, Korea. Korean J Food Sci Anim Resour. 2013;33:403-10.
  25. Lorenzo JM, Gomez M. Shelf life of fresh foal meat under MAP, overwrap and vacuum packaging conditions. Meat Sci. 2012;92:610-8.
  26. Holds G, Pointon A, Lorimer M, Kiermeier A, Raven G, Sumner J. Microbial profiles of carcasses and minced meat from kangaroos processed in South Australia. Int J Food Microbiol. 2008;123:88-92.
  27. Gill CO, Jones T. Microbiological sampling of carcasses by excision or swabbing. J Food Protec. 2000;63:167-73.
  28. Moriartya EM, McEvoya JM, Duffya G, Sheridana JJ, Blairb IS, McDowell DA. Development of a novel method for isolating and detecting Cryptosporidium parvum from lean and fat beef carcass surfaces. Meat Sci. 2004;21:275-82.