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Directed Differentiation of Pluripotent Stem Cells by Transcription Factors

  • Oh, Yujeong (Department of Life Sciences, Pohang University of Science and Technology (POSTECH)) ;
  • Jang, Jiwon (Department of Life Sciences, Pohang University of Science and Technology (POSTECH))
  • Received : 2018.11.28
  • Accepted : 2019.02.25
  • Published : 2019.03.31

Abstract

Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have been used as promising tools for regenerative medicine, disease modeling, and drug screening. Traditional and common strategies for pluripotent stem cell (PSC) differentiation toward disease-relevant cell types depend on sequential treatment of signaling molecules identified based on knowledge of developmental biology. However, these strategies suffer from low purity, inefficiency, and time-consuming culture conditions. A growing body of recent research has shown efficient cell fate reprogramming by forced expression of single or multiple transcription factors. Here, we review transcription factor-directed differentiation methods of PSCs toward neural, muscle, liver, and pancreatic endocrine cells. Potential applications and limitations are also discussed in order to establish future directions of this technique for therapeutic purposes.

Table 1. Transcription factor-directed PSC differentiation toward neurons

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Table 2. Transcription factor-directed PSC differentiation toward pancreatic β-cells

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Table 3. Transcription factor-directed PSC differentiation toward skeletal and cardiac muscle cells

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Table 4. Transcription factor-directed PSC differentiation toward hepatocytes

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Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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