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Neuropeptide Signaling Regulates Pheromone-Mediated Gene Expression of a Chemoreceptor Gene in C. elegans

  • Park, Jisoo (Department of Brain and Cognitive Sciences, DGIST) ;
  • Choi, Woochan (Department of Brain and Cognitive Sciences, DGIST) ;
  • Dar, Abdul Rouf (Department of Chemistry, University of Florida) ;
  • Butcher, Rebecca A. (Department of Chemistry, University of Florida) ;
  • Kim, Kyuhyung (Department of Brain and Cognitive Sciences, DGIST)
  • Received : 2018.09.18
  • Accepted : 2018.10.17
  • Published : 2019.01.31

Abstract

Animals need to be able to alter their developmental and behavioral programs in response to changing environmental conditions. This developmental and behavioral plasticity is mainly mediated by changes in gene expression. The knowledge of the mechanisms by which environmental signals are transduced and integrated to modulate changes in sensory gene expression is limited. Exposure to ascaroside pheromone has been reported to alter the expression of a subset of putative G protein-coupled chemosensory receptor genes in the ASI chemosensory neurons of C. elegans (Kim et al., 2009; Nolan et al., 2002; Peckol et al., 1999). Here we show that ascaroside pheromone reversibly represses expression of the str-3 chemoreceptor gene in the ASI neurons. Repression of str-3 expression can be initiated only at the L1 stage, but expression is restored upon removal of ascarosides at any developmental stage. Pheromone receptors including SRBC-64/66 and SRG-36/37 are required for str-3 repression. Moreover, pheromone-mediated str-3 repression is mediated by FLP-18 neuropeptide signaling via the NPR-1 neuropeptide receptor. These results suggest that environmental signals regulate chemosensory gene expression together with internal neuropeptide signals which, in turn, modulate behavior.

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Fig. 1. str-3 repression upon crude pheromone exposure is imposed from egg to the L1 stage.

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Fig. 2. Exposure to ascaroside pheromone components causes str-3 repression via pheromone receptors in L1 larvae and adults.

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Fig. 3. str-3 expression is not modulated by the quality or the quantity of food or ambient temperature.

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Fig. 4. FLP-18 neuropeptide and NPR-1 neuropeptide receptor are required for pheromone-mediated str-3 repression.

Acknowledgement

Supported by : National Research Foundation of Korea, Ministry of Science, ICT and Future Planning

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