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The Effect of Dilution with 10% Dextrose Solution on the Potency of Botulinum Toxin A

Ham, Jong Wook;Kwon, Jeong-Seung;Cho, Eunae Sandra;Choi, Jong Hoon

  • Received : 2019.01.18
  • Accepted : 2019.02.02
  • Published : 2019.03.30

Abstract

Purpose: The aim of this study was to compare the potency-stabilizing effects of two different diluents of botulinum toxin A (10% dextrose solution and 0.9% saline). Methods: A mouse lethality bioassay was undertaken. Ninety mice were divided into experimental and control groups which received varying dosages in subgroups of 10. The experimental group was injected with botulinum toxin A diluted with 10% dextrose solution and the control group was injected with botulinum toxin A diluted with 0.9% saline. A 72 hours after intraperitoneal injection, the number of dead mice was counted to confirm median lethal dose (LD50) of each group. Results: The value of LD50 in the experimental group was approximately 0.131 mL (1.31 U) and the value of LD50 in the control group was approximately 0.107 mL (1.07 U). The potency preservation rate of the experimental group was estimated to be 93.5% and that of the control group was estimated to be 76.3%. Conclusions: Dilution with 10% dextrose solution displayed less potency loss than 0.9% saline.

Keywords

Botulinum toxins;Type A;Glucose;Saline solution