A new approach for detoxification of landfill leachate using Trametes trogii

  • Smaoui, Yosr (Laboratory of Environmental Engineering and Eco Technology, National School of Engineers of Sfax, University of Sfax) ;
  • Fersi, Mariem (Algae Biotechnology Unit, National School of Engineers of Sfax, University of Sfax) ;
  • Mechichi, Tahar (Laboratory of Enzymatic Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax) ;
  • Sayadi, Sami (Environmental Bioprocesses Laboratory, LMI Cosys-Med, Centre of Biotechnology of Sfax) ;
  • Bouzid, Jalel (Laboratory of Environmental Engineering and Eco Technology, National School of Engineers of Sfax, University of Sfax)
  • Received : 2018.01.05
  • Accepted : 2018.07.12
  • Published : 2019.03.31


Landfill leachate constitutes one of the most polluting wastewaters. Their treatment was considered difficult due to the presence of high concentration of organic matter, ammonia, toxic organic compounds and heavy metals. Biological processes were found to be effective in several cases, but they are limited by the presence of inhibitory compounds in leachate. In this study we develop a biological process for the leachate biodetoxification using Trametes trogii (T. trogii; CLBE55). Results show that laccase activity, mycelia growth and chemical oxygen demand (COD) removal efficiencies varied depending on the leachate and ammonium concentration. Indeed T. trogii was able to grow in the presence of low concentration of landfill leachate of 10 and 30%. In fact, the biomass produced was 4.7 and 3.7 g/L, respectively leading to a COD removal of 66 and 53%, respectively. However, when the concentration of the introduced leachate exceeds 30%, the treatment efficiency and particularly the COD removal decreases to reach 15% at 100% leachate. The effect of the ammonia was also studied and results showed that the addition of 5 g/L of ammonia inhibited totally the production of laccase and the COD removal.


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