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Effects of Nitrogen and Phosphorus Starvation on Growth and Fatty Acid Production in Newly Isolated Two Freshwater Green Microalgae from Nakdonggang River

낙동강 수계에서 분리한 녹조류 2종의 질소와 인의 결핍에 따른 생장 및 지방산 변화 연구

  • Yim, Kyung June (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Park, Hanwool (Department of Marine Science & Biological Engineering, Inha University) ;
  • Lee, Chang Soo (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Jo, Bok Yeon (Bioresources Industrialization Support Department, Nakdonggang National Institute of Biological Resources) ;
  • Nam, Seung Won (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Lee, Choul-Gyun (Department of Marine Science & Biological Engineering, Inha University) ;
  • Kim, Z-Hun (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
  • 임경준 (국립낙동강생물자원관 미생물연구실) ;
  • 박한울 (인하대학교 해양과학.생물공학과) ;
  • 이창수 (국립낙동강생물자원관 미생물연구실) ;
  • 조복연 (국립낙동강생물자원관 산업화지원실) ;
  • 남승원 (국립낙동강생물자원관 미생물연구실) ;
  • 이철균 (인하대학교 해양과학.생물공학과) ;
  • 김지훈 (국립낙동강생물자원관 미생물연구실)
  • Received : 2019.11.21
  • Accepted : 2019.12.12
  • Published : 2019.12.31

Abstract

In this study, effects of nitrogen (N) and phosphorus (P) starvation on the cell growth and fatty acid (FA) production of newly isolated freshwater microalgae were investigated. The microalgae were identified as Chlorella sp. and Parachlorella sp. through 18S rRNA sequencing. Optimal culture temperature and light intensity were investigated using a high-throughput photobioreator, and the result was validated in 0.5 L bubble column photobioreactors using BG-11 without NaNO3 and/or K2HPO4. Under nutrient starvation conditions, total FA contents of the microalgae were significantly changed rather than FA composition. Starvation of both N and P was most effective for increasing FA contents in Parachlorella sp (24.4±0.1%) whereas highest FA contents (42.6±1.8%) was achieved when only P was starved in Chlorella sp. among tested conditions. These results suggest an effective strategy for increasing FA production from microalgae using appropriate nutrient starvation.

Acknowledgement

Supported by : 국립낙동강생물자원관

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