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Detection of Sugar Beet (Beta vulgaris) - Specific Gene from Honey Made by Sugar of Sugar Beet

사탕무(Beta vulgaris) 설탕으로 제조된 사양꿀에서 사탕무 고유 유전자의 검출

  • Kim, Somin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Byounghee (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Moonjung (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Kim, Jungmin (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Truong, A Tai (Department of Life Science, College of Fusion Science, Kyonggi University) ;
  • Yoon, Byoungsu (Department of Life Science, College of Fusion Science, Kyonggi University)
  • 김소민 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • 김병희 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • 김문정 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • 김정민 (경기대학교 융합과학대학 바이오융합학부 생명과학전공) ;
  • ;
  • 윤병수 (경기대학교 융합과학대학 바이오융합학부 생명과학전공)
  • Received : 2018.08.31
  • Accepted : 2018.09.21
  • Published : 2018.09.30

Abstract

We could detect the specific gene of sugar beet (Beta vulgaris) from honey produced by sugar of sugar beet using the ultra-rapid real-time PCR (UR-qPCR). In our knowledge, it is the first report in the world that PCR detection of sugar beet-specific gene from adulterated honey. Through extracting the DNA (using CTAB method) from sugar and honey of sugar beet and using the UR-qPCR, sugar beet-specific DNA sequence could be amplified quantitatively until $10^0$ molecules of initial template. By using nested PCR and DNA sequencing, its specificity was confirmed. DNA sequence was matched 100% with mitochondria gene of sugar beet. This finding that trace DNA in adulterated honey could be genetically analysed, would be used as a decisive evidence for the authenticity test of honey.

Acknowledgement

Supported by : 농촌진흥청, 농림수산식품기술기획평가원, 중소기업청

References

  1. 김정민, 임수진, Truong A Tai, 홍기정, 윤병수. 2017. 초고속 유전자 증폭법을 이용한 벌집꼬마밑빠진벌레(Aethina tumida)의 신속한 검출 기법 개발. J. Apiculture 32: 119-131.
  2. 유응철, 공영건, 윤병수. 2010. 불량꿀 검사를 위한 개선된 분석 방법에 관한 연구. J. Apiculture 25: 63-76.
  3. 윤병수, 한상훈, 임희영. 2014. 분자생물학 연구방법론 IV. 경기대학교 출판부. 95-97p.
  4. Bertelli, D., M. Lolli, G. Papotti, L. Bortolotti, G. Serra, and M. Plessi. 2010. Detection of adulteration by sugar syrups using one-dimensional and two-dimensional highresolution Nuclear Magnetic Resonance. J. Agric. Food Chem. 58: 8495-8501.
  5. Boffo, E. F., L. A. Tavares, A. C. T. Tobias, M. M. C. Ferreira and A. G. Ferreira. 2012. Identification of components of Brazilian honey by H NMR and classification of its botanical origin by chemometric methods. LWT - Food Sci. Technol. 49: 55-63.
  6. Elflein, L. and K. Raezke. 2008. Improved detection of honey adulteration by measuring differences between $^{13}C$/$^{12}C$ stable carbon isotope ratios of protein and sugar compounds with a combination of elemental analyser e isotope ratio mass spectrometry and liquid chromatography - isotope ratio mass spectrometry (${\delta}^{13}C$EA/ LC-IRMS). Apidologie 39: 574-587.
  7. Jain S. A., F. T. d. Jesus, G. M. Marchioro and E. D. d. Araujo. 2013. Extraction of DNA from honey and its amplification by PCR for botanical identification. Food Sci. Technol. Campinas 33: 753-756.
  8. Ohmenhaeuser, M., Y. B. Monakhova, T. Kuballa and D. W. Lachenmeier. 2013. Qualitative and quantitative control of honeys using NMR spectroscopy and chemometrics. Analytical Chemistry 2013: 1-9.
  9. Riberio, R. d. O. R., E. T. Marsico, C. d. S. Carneiro, M. L. G. Monteiro, C. C. Junior and E. F. O. d. Jesus. 2014. Detection of honey adulteration of high fructose corn syrup by low field Nuclear Magnetic Resonance (LF1H NMR). J. Food Eng. 135: 39-43.
  10. Rios-Corripio, M. A., M. Rojas-Lopez and R. Delgado-Macuil. 2012. Analysis of adulteration in honey with standard sugar solutions and syrups using attenuated total reflectance-Fourier transform infrared spectroscopy and multivariate methods. CyTA - J. Food 10: 119-122.
  11. Sobrino-Gregorio, L., S. Vilanova, J. Prohens and I. Esriche. 2018. Detection of honey adulteration by conventional and real-time PCR. Food control 95: 57-62.
  12. Tosun, M. 2013. Detection of adulteration in honey samples added various sugar syrups with $^{13}C$/$^{12}C$ isotope ratio analysis method. Food chem. 138: 1629-1632.