Inhibition of 5α-reductase of de novo Generation of Short Anti-oxidant Peptides

  • Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University) ;
  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
  • Received : 2018.07.11
  • Accepted : 2018.10.01
  • Published : 2018.09.30


This study aims to investigate the biological activities related to hair loss of short anti-oxidant peptides (DK peptides) $5{\alpha}$-reductase inhibition and anti-oxidation. The series of DK peptides were generated amphipathic helical properties using leucines, lysines and tryptophan residues. Cell viability and free radical scavenging activities were performed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The DK peptides were investigated for inhibitory activity against $5{\alpha}$-reductase. Antioxidant activities were determined by means of, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. All peptides could inhibit $5{\alpha}$-reductase in lipopolysaccharide-stimulated macrophage. In conclusion, DK peptides was suggested as the most attractive ingredients for improving hair loss, because of the high inhibitory against $5{\alpha}$-reductase inhibition.


Supported by : Korea Institute for Advancement of Technology (KIAT)


  1. Ahn SY, Pi LQ, Hwang ST, Lee WS. Effect of IGF-I on hair growth is related to the anti-apoptotic effect of IGF-I and up-regulation of PDGF-A and PDGF-B. Annals of Dermatology. 2012. 24: 26-31.
  2. Amory JK, Wang C, Swerdloff RS, Anawalt BD, Matsumoto AM, Bremner WJ, Walker SE, Haberer LJ, Clark RV. The effect of 5alpha-reductase inhibition with dutasteride and finasteride on semen parameters and serum hormones in healthy men. J Clin Endocrinol Metab. 2007. 92: 1659-1665.
  3. Cotsarelis G, Millar SE. Towards a molecular understanding of hair loss and its treatment. Trends in Molecular Medicine. 2001. 7: 293-301.
  4. Dawber RPR. Aetiology and pathophysiology of hair loss. Dermatology. 1987. 175: 23-28.
  5. Dmytriw AA, Morzycki W, Green PJ. Prevention of Alopecia in Medical and Interventional Chemotherapy Patients. J Cutan Med Surg. 2015. 19: 11-16.
  6. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrite in biological fluids. Analytical Biochemistry. 1982. 126: 131-138.
  7. Huang KP, Mullangi S, Guo Y, Qureshi AA. Autoimmune, atopic, and mental health comorbid conditions associated with alopecia areata in the United States. JAMA Dermatol. 2013. 149: 789-794.
  8. Nanjo F, Goto K, Seto R, Suzuki M. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Biology And Medicine. 1996. 21: 895-902.
  9. Kwack MH, Ahn JS, Kim MK, Kim JC, Sung, YK. Dihydrotestosterone-inducible IL-6 inhibits elongation of human hair shafts by suppressing matrix cell proliferation and promotes regression of hair follicles in mice. Journal of Investigative Dermatology. 2012. 132: 43-49.
  10. Matsuda H, Yamazaki M, Matsuo K, Asanuma Y, Kubo M. Antiandrogenic Activity of Myricae Cortex-Isolation of Active Constituents from Bark of Myrica rubra. Biological and Pharmaceutical Bulletin. 2001. 24: 259-263.
  11. Naito A, Midorikawa T, Yoshino T, Ohdera M. Lipid peroxides induce early onset of catagen phase in murine hair cycles. International Journal of Molecular Medicine. 2008. 22: 725.
  12. Otberg N, Finner AM, Shapiro J. Androgenetic alopecia. Endocrinology Metabolism Clinics of North America. 2007. 36: 379 -398.
  13. Paus R, Cotsarelis G. The biology of hair follicles. N Engl J Med 1999. 341: 491-497.
  14. Stough D, Stenn K, Haber R, Parsley WM, Vogel JE, Whiting DA. Washenik, K. Psychological effect, pathophysiology, and management of androgenetic alopecia in men. Mayo Clinic Proceedings. 2005. 80: 1316-1322.
  15. Traish AM, Hassani J, Guay AT, Zitzmann M, Hansen ML. Adverse side effects of 5alpha-reductase inhibitors therapy: persistent diminished libido and erectile dysfunction and depression in a subset of patients. J Sex Med. 2011. 8: 872-884.
  16. Yim E, Nole KL, Tosti A. 5alpha-Reductase inhibitors in androgenetic alopecia. Curr Opin Endocrinol Diabetes Obes. 2014. 21:493-498.
  17. Whiting DA. Possible mechanisms of miniaturization during androgenetic alopecia or pattern hair loss. Journal of the American Academy of Dermatology. 2001. 45: 81-86.
  18. Wood JM, Decker H, Hartmann H, Chavan B, Rokos H, Spencer JD, Hasse S, Thornton MJ, Shalbaf M, Paus R. Senile hair graying: $H_{2}O_{2}$-mediated oxidative stress affects human hair color by blunting methionine sulfoxide repair. The FASEB Journal. 2009. 23: 2065-2075.