DOI QR코드

DOI QR Code

Production of HCN, Weed Control Substance, by Pseudomonas koreensis and its Plant Growth-Promoting and Termiticidal Activities

Pseudomonas koreensis에 의한 잡초제어활성물질인 HCN 생성과 이 균주의 식물성장 촉진 및 흰개미 살충 활성

  • Yoo, Ji-Yeon (Department of Life Science & Environmental Biochemistry/Life and Industry Convergence Institute, Pusan National University) ;
  • Jang, Eun-Jin (Department of Life Science & Environmental Biochemistry/Life and Industry Convergence Institute, Pusan National University) ;
  • Park, Soo-Yeun (Department of Life Science & Environmental Biochemistry/Life and Industry Convergence Institute, Pusan National University) ;
  • Son, Hong-Joo (Department of Life Science & Environmental Biochemistry/Life and Industry Convergence Institute, Pusan National University)
  • 유지연 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 장은진 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 박수연 (부산대학교 생명환경화학과 및 생명산업융합연구원) ;
  • 손홍주 (부산대학교 생명환경화학과 및 생명산업융합연구원)
  • Received : 2018.05.04
  • Accepted : 2018.06.16
  • Published : 2018.09.30

Abstract

To develope a microbial weed control agent, HCN-producing bacteria were isolated, and their characteristics were investigated. A selected strain of WA15 was identified as Pseudomonas koreensis by morphological, cultural, biochemical and 16S rRNA gene analyses. The conditions for HCN production was investigated by a One-Variable-at-a-Time (OVT) method. The optimal HCN production conditions were tryptone 1%, glycine 0.06%, NaCl 1%, and an initial pH and temperature of 5.0 and $30^{\circ}C$, respectively. The major component for HCN production was glycine. Under optimal conditions, HCN production was about 3 times higher than that of the basal medium. The WA15 strain had physiological activities, such as indoleacetic acid that was associated with the elongation of plant roots and siderophore and ammonification inhibiting fungal growth, and produced hydrolytic enzymes, such as cellulase, pectinase and lipase. The strain was able to inhibit the growth of phytopathogenic fungi, such as Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum, by the synergistic action of volatile HCN and diffusible antimicrobial compounds. A microscopic observation of R. solani that was teated with the WA15 strain showed morphological abnormalities of fungal mycelia, which could explain the role of the antimicrobial metabolites that were produced by the WA15 strain. The volatile HCN produced by the WA15 strain was also found to have insecticidal activity against termites. Our results indicate that Pseudomonas koreensis WA15 can be applied as a microbial agent for weed control and also as a termite repellent. Furthermore, it could be applied as a microbial termiticidal agent to replace synthetic insecticides.

Keywords

Antifungal activity;HCN;Pseudomonas koreensis;Termite;Weed control

References

  1. Astrom, B., Gerhardson, B., 1988, Differential reactions of wheat and pea genotypes to root inoculation with growth-affecting rhizosphere bacteria, Plant Soil, 109, 263-269. https://doi.org/10.1007/BF02202093
  2. Bakker, A. W., Schippers, B., 1987, Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp-mediated plant growth-stimulation, Soil Biol. Biochem. 19, 451-457. https://doi.org/10.1016/0038-0717(87)90037-X
  3. Barrow, G. I., Felthanm, R. K. A., 1993, Cowan and Steel's manual for the identification of medical bacteria, Cambridge University Press, New York, 94-116.
  4. Castric, P. A., 1983, Hydrogen cyanide production by Pseudomonas aeruginosa at reduced oxygen levels, Can. J. Microbiol., 29, 1344-1349. https://doi.org/10.1139/m83-209
  5. Chandra, S., Choure, K., Dubey, R. C., Maheshwari, D. K., 2007, Rhizosphere competent Mesorhizobium loti MP6 induces root hair curling, inhibits Sclerotinia sclerotiorum and enhances growth of Indian mustard (Brassica campestris), Braz. J. Microbiol., 38(1), 124-130. https://doi.org/10.1590/S1517-83822007000100026
  6. Choi, J. S., Kim, Y. S., Kim, J. D., Kim, H. J., Ko, Y. K., Park, K. W., Moon, S. S., 2017, Herbicidal characteristics of soil bacteria Actinomycetes G-0299 to southern crabgrass, Weed Turf. Sci., 6, 212-221. https://doi.org/10.5660/WTS.2017.6.3.212
  7. Dye, R., Pal, K. K., Bhatt, D. M., Chauhan, S. M., 2004, Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria, Microbiol. Res., 159, 371-394. https://doi.org/10.1016/j.micres.2004.08.004
  8. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R., Phillips, G. B., 1981, Manual of methods for general bacteriology, American Society for Microbiology, Washington, D.C., 285-392.
  9. Getha, K., Vikineswary, S., 2002, Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f.sp. cubense race 4: Indirect evidence for the role of antibiosis in the antagonistic process, J. Ind. Microbiol. Biotechnol., 28, 303-310. https://doi.org/10.1038/sj.jim.7000247
  10. Grossman, K., Kwiatkowski, J., 1995, Evidence for a causative role of cyanide, derived from ethylene biosynthesis, in the herbicidal mode of action of quinclorac in barnyard grass, Pestic. Biochem. Physiol., 51, 150-160. https://doi.org/10.1006/pest.1995.1015
  11. Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., Williams, S. T., 1994, Bergey's Manual of Determinative Bacteriology, 9th ed., The Williams and Wilkins Co., Baltimore.
  12. Kamei, A., Dolai, A. K., Kamei, A., 2014, Role of hydrogen cyanide secondary metabolite of plant growth promoting rhizobacteria as biopesticides of weeds, Global J. Sci. Front. Res., 14, 108-112.
  13. Kremer, R. J., Kennedy, A. C., 1996, Rhizobacteria as biocontrol agents of weeds, Weed Technol., 10, 601-609. https://doi.org/10.1017/S0890037X00040525
  14. Kremer, R. J., Souissi, T., 2001, Cyanide production by rhizobacteria and potential for suppression of weed seedling growth, Curr. Microbiol., 43, 182-186. https://doi.org/10.1007/s002840010284
  15. Lakshmi, V., Kumari, S., Singh, A., Prabha, C., 2015, Isolation and characterization of deleterious Pseudomonas aeruginosa KC1 from rhizospheric soils and its interaction with weed seedlings, J. King Saud Univ. Sci., 27, 113-119. https://doi.org/10.1016/j.jksus.2014.04.007
  16. Lee, I. Y., Park, T. S., Choi, J. S., Ko, Y. K., Park, K. W., Seo, H. A., 2016, Current status and perspectives of weed science in the world, Weed Turf. Sci., 5, 105-110. https://doi.org/10.5660/WTS.2016.5.3.105
  17. Matsuura, K., 2005, Distribution of termite egg-mimicking fungi ("termite balls") in Reticulitermes spp. (Isoptera: Rhinotermitidae) nests in Japan and the United States, Appl. Entomol. Zool., 40, 53-61. https://doi.org/10.1303/aez.2005.53
  18. Mayer, A. M., 1958, Determination of indole acetic acid by the Salkowsky Reaction, Nature, 182, 1670-1671. https://doi.org/10.1038/1821670a0
  19. Michaels, R., Hankes, L. V., Corpe, W.A., 1965, Cyanide formation from glycine by nonproliferating cells of Chromobacterium violaceum, Arch. Biochem. Biophys., 111, 121-125. https://doi.org/10.1016/0003-9861(65)90329-2
  20. Michelsen, C. F., Stougaard, P., 2012, Hydrogen cyanide synthesis and antifungal activity of the biocontrol strain Pseudomonas fluorescens In5 from Greenland is highly dependent on growth medium, Can. J. Microbiol., 58, 381-390. https://doi.org/10.1139/w2012-004
  21. Mishra, S., Upadhyay, R. M., Nautiyal, C. H., 2013, Unravelling the beneficial role of microbial contributors in reducing the allelopathic effects of weeds, Appl. Microbiol. Biotechnol., 97, 5659-5668. https://doi.org/10.1007/s00253-013-4885-y
  22. Owen, A., Zdor, R., 2001, Effect of cyanogenic rhizobacteria on the growth of velvetleaf (Abutilon theophrasti) and corn (Zea mays) in autoclaved soil and the influence of supplemental glycine, Soil Biol. Biochem., 33, 801-809. https://doi.org/10.1016/S0038-0717(00)00228-5
  23. Randviir, E. P., Banks, C. E., 2015, The latest developments in quantifying cyanide and hydrogen cyanide, Trends Analyt. Chem., 64, 75-85. https://doi.org/10.1016/j.trac.2014.08.009
  24. Schippers, B., Bakker, A. W., Bakker, P. A. H. M., Van Peer, R., 1990, Beneficial and deleterious effects of HCN-producing pseudomonads on rhizosphere interactions, Plant Soil, 129, 75-83. https://doi.org/10.1007/BF00011693
  25. Selvakumar, G., Joshi, P., Nazim, S., Mishra, P. K., Bisht, J. K., Gupta, H. S., 2009, Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude Himalayan rhizosphere, Biologia, 64, 2, 239-245.
  26. Sulochana, M. B., Jayachandra, S. Y., Kumar, S. A., Parameshwar, A. B., Reddy, K. M., Dayanand, A., 2014, Siderophore as a potential plant growth-promoting agent produced by Pseudomonas aeruginosa JAS-25, Appl. Biochem. Biotechnol., 174, 297-308. https://doi.org/10.1007/s12010-014-1039-3
  27. Vassilev, N., Vassileva, M., 2003, Biotechnological solubilization of rock phosphate on media containing agro-industrial wastes, Appl. Microbiol. Biotechnol., 61, 435-440. https://doi.org/10.1007/s00253-003-1318-3
  28. Vyas, P., Rahi, P., Chadh, B. S., Gulati, A., 2014, Statistical optimization of medium components for mass production of plant growth-promoting microbial inoculant Pseudomonas trivialis BIHB 745 (MTCC5336), Ind. J. Microbiol., 54, 239-241. https://doi.org/10.1007/s12088-013-0425-9
  29. Ware, G. W., Whitacre, D. M., 2004, An Introduction to Herbicides, 2nd ed., A division of Meister Media Worldwide, Ohio.
  30. Zimbro, M. J., Power, D. A., Wilson, G. E., Johnson, J. A., 2009, Difco & BBL manual - Manual of microbiological culture media, Becton, Dickinson and Company, Maryland.