DOI QR코드

DOI QR Code

Investigation of Pharmacological Stability of Hyeonggaeyeongyotang Gagambang by Formulation Changes for Foaming Tablet

형개연교탕(荊芥連翹湯) 가감방(가감방)의 발포정 제형 변화에 따른 약리학적 안정성 연구

  • Cho, Nam Joon (Department of Biochemistry, Chungnam National University) ;
  • Gang, Seong Gu (Department of Biochemistry, Chungnam National University) ;
  • Kim, Ji Young (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyo Sang (Department of Health Administration, Joongbu University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University)
  • Received : 2018.05.29
  • Accepted : 2018.09.25
  • Published : 2018.09.30

Abstract

Objectives : Natural extracts have been extensively studied to replace single agent drugs that cause a variety of side effects. However, studies of changes to the formulation of natural extracts has not been nearly proceed. We aimed to investigate whether pharmacological stability of hyeonggaeyeongyotang gagambang (HYT) is altered by formulation changes for foaming tablet. Methods : In this study, we performed freeze - drying of HYT, which is known to have antioxidant and anti - inflammatory properties, and then changed the formulation by foaming. Results : As a result, the foaming reaction appeared normally when HYT foamed tablets were put into water, and almost all of the substances were dissolved in the aqueous solution. In addition, we confirmed using high-performance liquid chromatograph that the geniposide used as an indicator material of HYT was stable in most of the formulations. It was confirmed that the change of HYT formulation did not affect the antioxidant efficacy by the 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid assay. Moreover, quantitative real-time PCR confirmed that the inhibitory effect of HYT on IL-$1{\beta}$ mRNA expression induced by lipopolysaccharides treatment in murine macrophage RAW 264.7 cells was similar in the solution of foaming tablet. Conclusions : These results suggest that the materials with various pharmacological effects can be stably maintained even when the formulation is changed by the foaming action of HYT. Our results are expected to provide important basic knowledge on formulation changes using various natural extracts.

Acknowledgement

Supported by : 중소기업청

References

  1. Rao P, Knaus EE. Evolution of nonsteroidal antiinflammatory drugs (NSAIDs): cyclooxygenase (COX) inhibition and beyond. J Pharm Pharm Sci. 2008 ; 11(2) : 81-110. https://doi.org/10.18433/J3T886
  2. Huang JQ, Sridhar S, Hunt RH. Role of Helicobacter pylori infection and non-steroidal anti-inflammatory drugs in peptic-ulcer disease: a meta-analysis. Lancet. 2002 ; 359(9300) : 14-22. https://doi.org/10.1016/S0140-6736(02)07273-2
  3. Winkelmayer WC, Waikar SS, Mogun H, Solomon DH. Nonselective and cyclooxygenase-2-selective NSAIDs and acute kidney injury. Am J Med. 2008 ; 121(12) : 1092-8. https://doi.org/10.1016/j.amjmed.2008.06.035
  4. Kong JH Jeungbomanbyeonghoechun hagwon. Seoul : Iljungsa 1994 : 12-4.
  5. Kim MJ, Lee JR, Kim SC, Jee SY. Inhibitory Effect of Hyeonggaeyeongyo-tang Water Extract on production of Nitric Oxide, IL-6 and Expression of iNOS, COX-2 in LPS - Activated Raw 264.7 Cells. Korean J. physiology & pathology. 2007 ; 21(2) : 491-7.
  6. Gang SG, Cho NJ, Kim JY, Han HS, Kim KK. Investigation of Antimicrobial and Anti-inflammatory Activities of the Hyeonggaeyeongyotang Gagambang. Kor. J. Herbol. 2018;33(4) :35-41. https://doi.org/10.6116/KJH.2018.33.4.35
  7. Shi Q, Cao J, Fang L, Zhao H, Liu Z, Ran J, Zheng X, Li X, Zhou Y, Ge D, Zhang H, Wang L, Ran Y, Fu J. Geniposide suppresses LPS-induced nitric oxide, PGE2 and inflammatory cytokine by downregulating NF-${\kappa}B$, MAPK and AP-1 signaling pathways in macrophages.Int Immunopharmacol. 2014 ; 20(2) : 298-306. https://doi.org/10.1016/j.intimp.2014.04.004
  8. Jagdeo J, Adams L, Lev-Tov H, Sieminska J, Michl J, Brody N. Dose-dependent antioxidant function of resveratrol demonstrated via modulation of reactive oxygen species in normal human skin fibroblasts in vitro. J Drugs Dermatol. 2010 ; 9(12) : 1523-6.
  9. Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, LeeYC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY,. Boncho-Hak. Seoul: Young-Lim Press. 2007 : 157, 163, 207, 216, 345, 393, 447, 496, 583, 629, 634.
  10. Miao EA, Leaf IA, Treuting PM, Mao DP, Dors M, Sarkar A, Warren SE, Wewers MD, Aderem A. Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria. Nat Immunol. 2010 ; 11(12) : 1136-42. https://doi.org/10.1038/ni.1960
  11. Shao HB, Chu LY, Shao MA, Jaleel CA, Mi HM. Higher plant antioxidants and redox signaling under environmental stresses. Send to C R Biol. 2008 ; 331(6) : 433-41. https://doi.org/10.1016/j.crvi.2008.03.011
  12. Sung YY, Lee AY, Kim HK. The Gardenia jasminoides extract and its constituent, geniposide, elicit antiallergic effects on atopic dermatitis by inhibiting histamine in vitro and in vivo. J Ethnopharmacol. 2014 ; 156 : 33-40. https://doi.org/10.1016/j.jep.2014.07.060
  13. Park JE, Lee JY, Kim HG, Hahn TR, Paik YS. Isolation and characterization of water-soluble intermediates of blue pigments transformed from geniposide of Gardenia jasminoides. J Agric Food Chem. 2002 ; 50(22) : 6511-4. https://doi.org/10.1021/jf020499b