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Current Methods of Circulating Tumor Cell Detection

순환종양세포 검출 기술

  • Lim, Minji (Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology) ;
  • Cho, Yoon-Kyoung (Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology)
  • 임민지 (울산과학기술원 생명과학부 생명공학과) ;
  • 조윤경 (울산과학기술원 생명과학부 생명공학과)
  • Received : 2018.03.28
  • Accepted : 2018.05.02
  • Published : 2018.09.30

Abstract

Liquid biopsy, the analysis of circulating biomarkers from peripheral blood, such as circulating tumor cells (CTCs) and circulating tumor DNA, and exosomes, offers a less invasive, new source of cancer-derived materials that may reflect the status of the disease better and thereby contribute to personalized treatment. Recent advances in microfluidics and molecular analysis technologies have resulted in greatly improved CTC enumeration and detection. In this article, we review commercially available technologies used to isolate CTCs from peripheral blood, including immunoaffinity and label-free, physical property-based isolation methods. Although enormous technological progress has been made, especially within the last decade, only a few CTC detection methods have been approved for routine clinical use. Here, we provide an overview of the current CTC isolation methods and examples of their potential application for early diagnosis, prognosis, treatment monitoring, and prediction of resistance to cancer therapy. Furthermore, the challenges that remain to be addressed before such tools are implemented for routine use in clinical settings are discussed.

Acknowledgement

Supported by : Ministry of Health & Welfare

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