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Sutdy of Appropriate Media Selection and Early Life Cycle of Marine Free-Living Nematodes, Enoplolaimus sp. (Enoplida: Thoracostomopsidae) and Bathylaimus sp. (Enoplida: Tripyloididae)

해양 자유생활형 선충류 Enoplolaimus sp. (Enoplida: Thoracostomopsidae)와 Bathylaimus sp. (Enoplida: Tripyloididae)의 배양용 적합배지 선정 및 초기 생활사 연구

  • 신아영 (한국해양과학기술원 생태기반연구센터) ;
  • 김동성 (한국해양과학기술원 생태기반연구센터) ;
  • 강태욱 (한국해양과학기술원 생태기반연구센터) ;
  • 오제혁 (한국해양과학기술원 생태기반연구센터) ;
  • 이지민 (한국해양과학기술원 생태기반연구센터) ;
  • 홍재상 (한국연안환경생태연구소)
  • Received : 2016.11.18
  • Accepted : 2018.07.06
  • Published : 2018.08.31

Abstract

In order to find the optimum culture condition for marine free-living nematoda in the laboratory, various agar media were developed and experiments were carried on nematodes. Nematodes have collected from the bottom of the sandy sediments' surface layer (about 5cm sediment) of Taean Mallipo beach's intertidal zone. Especially, with regard to agar medium, Killian medium was transformed slightly, density of agar had made a difference and this agar medium(height 2.0 mm on diameter 60 mm Petri dish) was divided. It was mixed with 5 different species of microorganism as nematodes' live food and added each culture medium. Five individuals of Enoplolaimus sp. on each culture medium were grown in a culture medium which was set on $20^{\circ}C$ and light blocked. Moreover, as a result of the optimum culture condition, 1.0% density of agar showed the highest survival rates (the average time of survival is 246.8 hours). On the other hand, the 0.4% density of agar adding Killian medium, bacto peptone and beef extract showed the lowest survival rates, which indicates the average time of survival is 27.6 hours. About Bathylaimus sp., on Killian medium's 1.0% density of agar(no feeding amount) showed the highest survival rates, which connects that the hatching rate 94.7% after 99.5 hours and it laid 7 eggs averagely on the spawning amount and the hatching rate experiment.

Keywords

Namatoda;Culture;Life history;Agar media

Acknowledgement

Grant : 종합해양과학기지 구축 및 활용연구

Supported by : 해양수산과학기술진흥원

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