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Protective Effects of Portulaca oleracea L. Extract against Matrix Metalloproteinase Production and Reactive Oxygen Species Generation Induced by Ultraviolet B Radiation in Human Keratinocytes

쇠비름 추출물의 UVB 자외선 조사에 의한 인간각질형성세포 손상에 대한 보호 효과

  • Oh, Jung Hwan (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Karadeniz, Fatih (Marine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University) ;
  • Lee, Jung Im (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Park, So Young (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University) ;
  • Seo, Youngwan (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)
  • 오정환 (신라대학교 의생명과학대학 식품영양학과) ;
  • 파티 카라데니즈 (신라대학교 해양식의약소재융합기술연구소) ;
  • 이정임 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 박소영 (신라대학교 의생명과학대학 식품영양학과) ;
  • 서영완 (한국해양대학교 해양과학기술대학 해양환경.생명과학부) ;
  • 공창숙 (신라대학교 의생명과학대학 식품영양학과)
  • Received : 2018.04.13
  • Accepted : 2018.05.17
  • Published : 2018.08.30

Abstract

Portulaca oleracea L. is an edible plant widely consumed in daily diet throughout Europe, Asia and America. In this study, protective effects of P. oleracea L. extracts against oxidative stress and matrix metalloproteinase (MMP) activity induced by ultraviolet B (UVB) radiation were investigated using HaCaT immortal human keratinocytes. In this context, the mRNA and protein productions of MMPs (MMP-1, -2, and -9) and type I procollagen, which are major markers of photoaging induced by UVB radiation in HaCaT keratinocytes, were evaluated. Furthermore, UVB-induced reactive oxygen species (ROS) generation and mRNA and protein expression levels of superoxide dismutase-1 (SOD-1), oxygenase-1 (OH-1), and nuclear factor-erythroid 2-related factor-2 (Nrf-2), all of which are associated with the antioxidant balance, were investigated. As shown by the results, UVB radiation induced ROS formation and led to increased production of MMPs and decreased collagen production in human keratinocytes, which resulted in skin photoaging or photodamage. The treatment with P. oleracea L. extracts downregulated MMP (MMP-1, -2, and -9) production and upregulated type I procollagen expression in UVB-induced HaCaT cells. Furthermore, treatment with the extracts decreased UVB-induced ROS generation and increased the expression of antioxidant enzymes, such as SOD-1 and OH-1, through the Nrf-2 pathway. Taken together, these results suggest that P. oleracea L. extracts could be a potential cosmeceutical agent for the prevention of skin photoaging or photodamage.

쇠비름(Portulaca oleracea.L)은 쇠비름과에 속하는 한해살이풀로서 리놀렌산과 같은 불포화지방산, 페놀성 화합물, 플라보노이드, 비타민 C, 미네랄 함량이 높은 것으로 보고되어 있다. 본 연구에서는 쇠비름 추출물을 이용하여 UVB를 조사한 인간각질형성세포에서 광노화 억제능을 확인하였다. Matrix metalloproteinases는 세포의 기질을 분해하는 효소로 MMP-1는 collagenase, MMP-2와 MMP-9는 gelatinases로 피부 진피층을 구성하는 type I collagen을 분해시키는데 영향을 미친다. UVB를 조사한 인간각질형성세포에서 쇠비름 추출물을 처리했을 때 MMP-1, -2, -9의 발현이 감소하였으며, type I procollagen의 발현은 증가하는 것으로 나타났다. 또한 쇠비름 추출물을 처리한 군에서 UV에 의한 ROS 생성이 감소하였는데 이는 Nrf-2의 활성화를 통한 항산화 인자 SOD-1과 OH-1의 발현 증가로 인해 세포내 ROS 생성이 감소한 것으로 사료된다. 따라서 본 연구 결과를 통해 쇠비름 추출물이 UVB를 조사한 인간각질형성세포에서 MMP 인자 및 항산화 인자의 발현 조절을 통해 광노화로부터의 세포 보호 능을 가지는 것을 확인하였으며 나아가 화장품 소재로서의 개발 가능성을 확인하였다.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

References

  1. Cesar, L. C., Elena, A. C., Mavil, L. C., Carlos, P. P., Elizebeth, A. S. and Laurence, A. M. 2012. Protein kinase and transcripition factors activation in response to UV-radiation of skin: Implications for carcinogenesis. Int. J. Mol. Med. Sci. 13, 142-172.
  2. Cho, Y. H., Bahuguna, A., Kim, H. H., Kim, D. I., Kim, H. J., Yu, J. M., Jung, H. G., Jang, J. Y., Kwak, J. H., Park, G. H., Kown, O. J., Cho, Y. J., An, J. Y., Jo C. R., Kang, S. C. and An, B. J. 2017. Potential effect of compounds isolated from Coffea arabica against UV-B induced skin damage by protecting fibroblast cells. J. Photochem. Photobiol. B. 174, 323-332. https://doi.org/10.1016/j.jphotobiol.2017.08.015
  3. Diego, S. C., Natalia, P., Carla, Z., Pagono, E., Karina, B. and Gustavo, Y. 2017. Heme oxygenase up-regulation under ultraviolet-B radiation in not epigenetically restricted and involves specific stress-related transcriptions factors. Redox Biol. 12, 549-557. https://doi.org/10.1016/j.redox.2017.03.028
  4. Erkan, N. 2012. Antioxidant activity and phenolic compounds of fractions from Portulaca oleracea L. Food Chem. 133, 775-781. https://doi.org/10.1016/j.foodchem.2012.01.091
  5. Fernando, P. M. D. J., Piao, M. J., Hewage, S. R. K. M., Kang, H. K., Yoo, E. S., Koh, Y. S., Ko, M. H., Ko, C. S., Byeon, S. H., Mun, S. R., Lee, N. H. and Hyun, J. M. 2016. Photo-protective effect of sargachromenol against UVB radiation- induced damage through modulating cellular antioxidant systems and apoptosis in human kerinocyte. Environ. Toxicol. Pharmacol. 43, 112-119. https://doi.org/10.1016/j.etap.2016.02.012
  6. Fridovich, I. 1978. The biology of oxygen radicals. Science 201, 875-880. https://doi.org/10.1126/science.210504
  7. Herouy, Y. 2004. The role of matrix metalloproteinases (MMPs) and their inhibitor in venous leg ulcer healing. Phlebolymphology 44, 231-243.
  8. Huang, C. C., Wu, W. B., Fang, J. Y., Chiang, H. S., Chen, S. K., Chen, B. H., Chen, Y. T. and Hung, C. F. 2007. (-)-Epicatechin-3-gallate, a green tea polyphenol is a potent agent against UVB-induced damage in HaCaT keratinocytes. Molecules 12, 1845-1858. https://doi.org/10.3390/12081845
  9. Jang, J. Y., Ye, B. R., Heo, S. J., Oh, C. H., Kang, D. H., Kim, J. H., Affan, A., Yoon, K. T., Choi, Y. U., Park, S. C., Han, S. H., Qian, Z. J., Jung, W. K. and Choi, I. W. 2012. Photo-oxidative stress by ultraviolet-B radiation and antioxidative defense of eckstolonol in human keratinocytes. Environ. Toxicol. Pharmacol. 34, 926-934. https://doi.org/10.1016/j.etap.2012.08.003
  10. Kikuchi, G., Yoshida, T. and Noguchi, M. 2005. Heme oxygenase and heme degradation. Biochem. Biophys. Res. Commun. 338, 558-567. https://doi.org/10.1016/j.bbrc.2005.08.020
  11. Kim, M. S., Park, Y. G., Lee, H. J., Lim, S. J. and Nho, C. W. 2015. Youngiasides A and c isolated from Younia denticulatum inhibit UVB-induced MMP expression and promote type I procollagen production via repression of MAPK/AP-1/NF-${\kappa}B$ and activation of AMPK/Nrf2 in HaCaT cells and human dermal fibroblast. J. Agric. Food Chem. 63, 5428-5438. https://doi.org/10.1021/acs.jafc.5b00467
  12. Lee, H. J., Lee, J. B., Lee, D. S. and Seo, Y. W. 2003. DPPH radical scavenging effect and in vitro lipid peroxidation inhibition by Portulaca oleracea. Kor. J. Biotechnol. Bioeng. 18, 165-169.
  13. Lei, X., Li,. J. Liu, B., Zhang, N. and Liu, H. 2015. Separation and identification of four new compounds with antibacterial activity from Portulaca oleracea L. Molecules 20, 16375-16387. https://doi.org/10.3390/molecules200916375
  14. Li, C. Y., Meng, Y. H., Ying, Z. M., Xu, N., Hao, D., Gao, M. Z., Zhang, W. J., Xu, L., Gao, Y. C. and Ying, X. X. 2016. Three novel alkaloids from Portulaca oleracea L. and their anti-inflammatory effects. J. Agric. Food Chem. 64, 5837-5844. https://doi.org/10.1021/acs.jafc.6b02673
  15. Lim, Y. Y. and Quah, E. P. L. 2007. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem. 103, 737-740.
  16. Liu, C., Vojnovic, D., Kochevar, I. E. and Jurkunas, U. V. 2016. UV-A irradiation activates Nrf2-regulated antioxidant defense and induced p53/caspase3-dependent apoptosis in corneal endothelial cells. Invest. Ophthalmol. Vis. Sci. 57, 2319-2327. https://doi.org/10.1167/iovs.16-19097
  17. Marines, M. D. 1997. The heme oxygenase system: A regulator of second messenger gases. Annu. Rev. Pharmacol. Toxicol. 37, 517-554. https://doi.org/10.1146/annurev.pharmtox.37.1.517
  18. Matsumura, Y. and Ananthaswamy, H. N. 2004. Toxic effects of ultraviolet radiation on the skin. Toxicol. Appl. Pharmacol. 195, 298-308. https://doi.org/10.1016/j.taap.2003.08.019
  19. Nagase, H., Visse, R. and Murphy, G. 2006. Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc. Res. 69, 562-573. https://doi.org/10.1016/j.cardiores.2005.12.002
  20. Nimse, S. B. and Pal, D. 2015. Free radicals, natural antioxidants, and their reaction mechanisms. Mol. Omics 5, 27986-28006.
  21. Oh, Y. R., Lim, H. W., Huang, Y. H., Kwon, H. S., Jin, C. D., Kim, K. H. and Lim, C. J. 2016. Attenuating properties of Agastache rugose leaf extract against ultraviolet-B-induced photoaging via up-regulating glutathione and superoxide dismuatase in a human keratinocyte cell line. J. Photochem. Photobiol. B. 163, 170-176. https://doi.org/10.1016/j.jphotobiol.2016.08.026
  22. Palaniswamy, U. R., McAvoy, R. J. and Bible, B. B. 2001. Stage of harvest and polyunsaturated essential fatty acid concentrations in Purslane (Portulaca oleracea) leaves. J. Agric. Food Chem. 49, 3490-3493. https://doi.org/10.1021/jf0102113
  23. Park, E. K., Ahn, S. R., Kim, D. H., Lee, E. W., Kwon, H. J., Kim, B. W. and Kim, T. H. 2014. Effects of unripen apple polyphenols on the expression of matrix metaproteinase-1 and type-1 procollagen in ultraviolet irradiated human skin fibroblast. J. Kor. Soc. Appl. Biol. Chem. 57, 49-455.
  24. Podhaisky, H. P., Riemschneider, S. and Wohlrab, W. A. 2002. UV light and oxidative damage of the skin. Pharmazie 57, 30-33.
  25. Radhakrishnan, R., Zakaria, M. N. M., Islam, M. W., Chen, H. B., Kamil, M., Chan, K. and Al-Attas, A. 2001. Nuropharmacological actions of Portulaca oleracea L v.sativa (Hawk). J. Ethnopharmacol. 76, 171-176. https://doi.org/10.1016/S0378-8741(01)00230-6
  26. Rittie, L. and Fisher, G. J. 2002. UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1, 705-720. https://doi.org/10.1016/S1568-1637(02)00024-7
  27. Schafer, M., Dutsch, S., Keller, U. A. D. and Werner, S. 2010. A central regulator of UV protection in the epidermis. Cell Cycle 9, 2917-2918. https://doi.org/10.4161/cc.9.15.12701
  28. Seo, S. H., Bae. G. S., Choi, S. B., Jo, I. J., Kim, D. G., Shin, J. Y., Song, H. J., Park, S. J. and Choi, M. O. 2014. The antioxidative and cytoprotective effect of Lonicerae japonicae flots water extracts on the ultraviolet (UV)B-induced human HaCaT keratinocytes. Kor. J. Herbology 29, 63-71. https://doi.org/10.6116/kjh.2014.29.6.63.
  29. Uddin, M. K., Juraimi, A. S., Ali, M. E. and Ismail, M. R. 2012. Evaluation of antioxidant properties and mineral composition of Purslane (Portulaca oleracea L.) at different growth stage. Int. J. Mol. Med. Sci. 13, 10257-10267. https://doi.org/10.3390/ijms130810257
  30. Wenk, J., Brenneisen, P., Meewes, C., Wlaschek, M., Peters, T., Blaudschun, R., Ma, W., Kuhr, L., Schneider, L. and Kohanek, S. 2001. UV-induced oxidative stress and photoaging. Curr. Probl. Dermatol. 29, 83-94.
  31. Xu, X., Yu, L. and Chen, G. 2006. Determination of flavonoids in Portulaca oleracea L. by capillary electrophoresis with electrochemical detection. J. Pharm. Biomed. Anal. 41, 493-499. https://doi.org/10.1016/j.jpba.2006.01.013