Effects of xylanase supplementation to wheat-based diets on growth performance, nutrient digestibility and gut microbes in weanling pigs

  • Dong, Bing (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Liu, Shaoshuai (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Wang, Chunlin (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Cao, Yunhe (State Key Laboratory of Animal Nutrition, China Agricultural University)
  • Received : 2017.11.30
  • Accepted : 2018.02.17
  • Published : 2018.09.01


Objective: This study was designed to investigate the effects of an Aspergillus sulphureus xylanase expressed in Pichia pastoris on the growth performance, nutrient digestibility and gut microbes in weanling pigs. Methods: A total of 180 weanling pigs (initial body weights were $8.47{\pm}1.40kg$) were assigned randomly to 5 dietary treatments. Each treatment had 6 replicates with 6 pigs per replicate. The experimental diets were wheat based with supplementation of 0, 500, 1,000, 2,000, and 4,000 U xylanase/kg. The experiment lasted 28 days (early phase, d 0 to 14; late phase, d 15 to 28). Results: In the early phase, compared to the control, average daily gain (ADG) was higher for pigs fed diets supplemented with xylanase and there was a quadratic response in ADG (p<0.05). In the entire phase, ADG was higher for the pigs fed 1,000 or 2,000 U/kg xylanase compared to the control (p<0.05). The gain to feed ratio was higher for pigs fed diets supplemented with 1,000 or 2,000 U/kg xylanase compared to the control (p<0.05). Increasing the amount of xylanase improved the apparent total tract digestibility of dry matter, crude protein, neutral detergent fiber, calcium, and phosphorus during both periods (p<0.05). Xylanase supplementation (2,000 U/kg) decreased the proportion of Lachnospiraceae (by 50%) in Firmicutes, but increased Prevotellaceae (by 175%) in Bacteroidetes and almost diminished Enterobacteriaceae (Escherichia-Shigella) in Proteobacteria. Conclusion: Xylanase supplementation increased growth performance and nutrient digestibility up to 2,000 U/kg. Supplementation of xylanase (2,000 U/kg) decreased the richness of gut bacteria but diminished the growth of harmful pathogenic bacteria, such as Escherichia-Shigella, in the colon.


  1. Choct M, Hughes RJ, Annison G. Apparent metabolisable energy and chemical composition of Australian wheat in relation to environmental factors. Aust J Agric Res 1999;50: 447-52.
  2. Wootton M, Acone L, Wills R, et al. Pentosan levels in Australian and north American feed wheats. Aust J Agric Res 1995;46:389-92.
  3. Li CX, Qiu CB, Jiang LN, et al. Research on the content of pentosane in wheat grain. J Triticeae Crops 2002;22:47-50.
  4. Simon O. The mode of action of NSP hydrolyzing enzymes in the gastrointestinal tract. J Anim Feed Sci 1998;7:115-23.
  5. Castanon JIR, Flores MP, Pattersson D. Mode of degradation of non-starch polysaccharides by feed enzyme preparations. Anim Feed Sci Technol 1997;68:361-5.
  6. Meng X. Improved nutrient utilization and growth performance of broiler chickens fed diets supplemented with multicarbohydrase enzyme preparations [PhD thesis]. Winnipeg, MB, Canada: University of Manitoba. 2005
  7. Pluske JR, Pethick DW, Hopwood DE, Hampson DJ. Nutritional influences on some major enteric bacterial diseases of pig. Nutr Res Rev 2002;15:333-71.
  8. Cao Y, Qiao J, Li Y, Lu W. De novo synthesis, constitutive expression of Aspergillus sulphureus ${\beta}$-xylanase gene in Pichia pastoris and partial enzymic characterization. Appl Microbiol Biotechnol 2007;76:579-85.
  9. Chen X, Cao Y, Ding Y, et al. Cloning, functional expression and characterization of Aspergillus sulphureus ${\beta}$-mannanase in Pichia pastoris. J Biotechnol 2007;128:452-61.
  10. NRC. Nutrient requirements of swine. Washington, DC, USA: National Academy Press; 2012.
  11. Fan MZ, Sauer WC. Determination of true ileal amino acid digestibility and the endogenous amino acid outputs associated with barley samples for growing-finishing pigs by the regression analysis technique. J Anim Sci 2002;80:1593-605.
  12. Thiex NJ, Anderson S, Gildemeister B. Crude fat, di-ethyl ether extraction, in feed, cereal grain, and forage (Randall/Soxtec/ submersion method): collaborative study. J AOAC Int 2003; 86:888-98.
  13. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97.
  14. Katherine RA, Carl JY, Angela K, et al. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes. ISME J 2013;7:1344-53.
  15. Ellis PR, Roberts FG, Low AG, Morgan LM. The effect of high-molecular-weight guar gum on net apparent glucose absorption and net apparent insulin and gastric inhibitory polypeptide production in the growing pig: relationship to rheological changes in jejunal digesta. Br J Nutr 1995;74:539-56.
  16. Hubener K, Vahjen W, Simon O. Bacterial responses to different dietary cereal types and xylanase supplementation in the intestine of broiler chicken. Arch Anim Nutr 2002;56:167-87.
  17. Choct M, Hughes RJ, Wang J, et al. Increased small intestinal fermentation is partly responsible for the antinutritive activity of non-starch polysaccharides in chickens. Br Poult Sci 1996; 37:609-21.
  18. Apajalahti J, Bedford M. Nutrition effects on the microflora of the GI tract. In Proceedings of the 19th Western Nutrition Conference. Saskatoon, SK, Canada: University of Saskachewan; 1998. pp. 60-8.
  19. Sterk A, Verdonk JM, Mul AJ, et al. Effect of xylanase supplementation to a cereal-based diet on the apparent faecal digestibility in weanling piglets. Livest Sci 2007;108:269-71.
  20. Yin YL, Mcevoy JDG, Schulze H, et al. Apparent digestibility (ileal and overall) of nutrients and endogenous nitrogen losses in growing pigs fed wheat (var. Soissons) or its by-products without or with xylanase supplementation. Livest Prod Sci 2000;62:119-32.
  21. Ricca DM, Ziemer CJ, Kerr BJ. Changes in bacterial communities from swine feces during continuous culture with starch. Anaerobe 2010;16:516-21.
  22. Louis P, Flint HJ. Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine. FEMS Microbiol Lett 2009;294:1-8.
  23. Zhang K, Dong X. Selenomonas bovis sp. nov., isolated from yak rumen contents. Int J Syst Evol Microbiol 2009;59:2080-3.
  24. Kajihara Y, Yoshikawa S, Cho Y, et al. Preferential isolation of Megasphaera elsdenii from pig feces. Anaerobe 2017;48:160-4.
  25. Wu GD, Chen J, Hoffmann C, et al. Linking long-term dietary patterns with gut microbial enterotypes. Science 2011;334:105-8.